García Carmona, Francisco

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García Carmona, Francisco

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Open Access
El legado del Catedrático Mario Honrubia García
(Universidad de Murcia, 2016) García Carmona, Francisco
Open Access
Estudio cinético de la fase de transición de los mecanismos iso ping pong bi bi e iso ordenado bi bi
(Murcia, Secretariado de Publicaciones e Intercambio científico, Universidad de Murcia, 1985) Gálvez Morillas, Jesús Ángel; Varón Castellanos, Ramón; García Cánovas, Francisco; García Carmona, Francisco; Facultad de Ciencias (Química y Matemáticas)
The kinetic behaviour during the transient phase of the Iso Ping Pong Bi Bi and Iso Ordered Bi Bi mechanisms has been studied and equations have been obtained which show the dependence with time of the products that are involved in those mechanisms. A procedure to obtain the rate constants is proposed.
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Propiedades cinéticas de la actividad hidroxilante de tirosinasa /Francisco García Carmona ; Directores J. A. Lozano Teruel, Fcº García Cánovas.
(Murcia : Universidad,, 1980) García Carmona, Francisco
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Study of oxyresveratrol complexes with insoluble cyclodextrin based nanosponges: Developing a novel way to obtain their complexation constants and application in an anticancer study
(Elsevier, 2019-12-17) Kumar Dhakarb, Nilesh; Bessonec, Federica; Musso, Giorgia; Cavallic, Roberta; Dianzanic, Chiara; Matencio Durán, Adrián; López Nicolás, José Manuel; García Carmona, Francisco; Bioquímica y Biología Molecular A
The complexation of the bioactive compound oxyresveratrol (OXY) with a polymer called cyclodextrin-based nanosponge (CD-NS) and its application was studied.A new methodology is used to calculate, an apparent inclusion complex constant (KFapp) between a ligand and CD-NSs. Moreover, the KFapp of resveratrol was also evaluated and compared. The complex of OXY with the nanosponge β-CDI 1:4, was studied in vitro using DSC, TGA and FTIR techniques and its drug loading and release behavior were studied. An in vitro digestion showed higher protection of OXY complexed than free OXY. The bioactivity enhancing capacity of OXY was also studied against prostate (PC-3) and colon (HT-29 and HCT-116) cancer cell lines, where it showed stronger cell viability inhibition than the free drug. The findings as a whole represent a new opportunity for studying the complexation of drugs in CD-NSs and the use of oxyresveratrol as an ingredient in nutraceutical products.
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Formation of carboxylated and decarboxylated betalains in ripening grains of Chenopodium quinoa by a dual dioxygenase
(Oxford University Press, Society for Experimental Biology, 2022-04-07) Henarejos Escudero, Paula; Martínez-Rodríguez, Pedro; Gomez-Pando, Luz Rayda; Gandía Herrero, Fernando; García Carmona, Francisco; Bioquímica y Biología Molecular "A"
Chenopodium quinoa (quinoa) is a pseudo-cereal that forms part of the cultural heritage of Andean countries, and its grains have high nutritional value and potential health benefits. Betalains are nitrogenous water-soluble pigments and bioactive molecules that contribute to these health-promoting properties. Betalains are restricted to plants of the order Caryophyllales, to which quinoa belongs. A new family of betalains has been discovered in the form of unconventional decarboxylated pigments. Here, we show that these pigments accumulate in ripening quinoa grains of fluorescent nature, and are putatively based on a dopamine-cleaving activity. This study describes for the first time the purification and molecular and functional characterization of a 4,5-dopamine extradiol dioxygenase enzyme from plants. It is a monomeric protein with a molecular mass of 34.5 kDa characterized by chromatography, electrophoresis, and time-offlight mass spectrometry. We demonstrate that this key enzyme has a dual function in a square-shaped biosynthetic pathway towards the formation of both carboxylated and decarboxylated pigments. Enzyme kinetic properties are characterized for the production of 6-decarboxy-betalamic acid and 3,4-dihydroxy-l-phenylalanine-derived betalamic acid, the two structural units of plant pigment in nature. The profile of multiple betalains present in quinoa grains has been reproduced in one-pot bioreactors containing the novel enzyme and two competing substrates
Open Access
Stilbenes: Characterization, bioactivity, encapsulation and structural modifications. A review of their current limitations and promising approaches
(Taylor and Francis Group, 2022-03-02) Navarro Orcajada, Silvia; Vidal Sánchez, Francisco José; Albadelejo Maricó, Lorena; Matencio Durán, Adrián; Conesa Valverde, Irene; López Nicolás, José Manuel; Vidal Sánchez, Francisco José; García Carmona, Francisco; Bioquímica y Biología Molecular A
Stilbenes are phenolic compounds naturally synthesized as secondary metabolites by the shikimate pathway in plants. Research on them has increased in recent years due to their therapeutic potential as antioxidant, antimicrobial, anti-inflammatory, anticancer, cardioprotective and anti-obesity agents. Amongst them, resveratrol has attracted the most attention, although there are other natural and synthesized stilbenes with enhanced properties. However, stilbenes have some physicochemical and pharmacokinetic problems that need to be overcome before considering their applications. Human clinical evidence of their bioactivity is still controversial due to this fact and hence, exhaustive basis science on stilbenes is needed before applied science. This review gathers the main physicochemical and biological properties of natural stilbenes, establishes structure-activity relationships among them, emphasizing the current problems that limit their applications and presenting some promising approaches to overcome these issues: the encapsulation in different agents and the structural modification to obtain novel stilbenes with better features. The bioactivity of stilbenes should move from promising to evident.
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Applications of cyclodextrins in food science. A Review
(Elsevier, 2020-08-25) Matencio Durán, Adrián; Navarro Orcajada, Silvia; López Nicolás, José Manuel; García Carmona, Francisco; Bioquímica y Biología Molecular A; Facultad de Biología
Background: The food industry is constantly attempting to develop better products that will have a positive effect on health (commonly known as functional foods). In this respect, cyclodextrins (CDs) could be of interest because they are tasteless, non-caloric and odourless molecules with several valuable characteristics, such as a capacity to separate chiral compounds and solubilize or stabilize bioactive compounds (BaC). Scope and approach: This review represents a revision of the state-of-the-art of CDs and their uses in the food industry. Key findings and conclusions: We analysed their metabolism and regulatory aspects of current applications of CDs: as carriers, for removing components, to produce or extract BaC, their use as nanosensors or in food packaging. We study how inclusion complexed are formed referring to the most common techniques and parameters Moreover, how inclusion complexes are formed will be studied with reference to the most common techniques and parameters. In conclusion, their applications in the food and other industries will increase in the coming years without a doubt.
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Comparative study of the antioxidant capacity of four stilbenes using ORAC, ABTS+, and FRAP Techniques
(Springer, 2017-03-17) Rodríguez-Bonilla, Pilar; Matencio Durán, Adrián; Gandía Herrero, Fernando; López Nicolás, José Manuel; García Carmona, Francisco; Bioquímica y Biología Molecular "A"
Researchers use several different analytical techniques, such as ORAC, ABTS·+, or FRAP, for measuring the antioxidant capacity of bioactive compounds. However, many authors do not take into account that these three techniques have different objectives. This contribution reports on the use of two types of tests to evaluate the antioxidant activity of four stilbene tests based first on the hydrogen atom transfer reaction (ORAC method) and the second on the single electron transfer reaction (ABTS·+ and FRAP assays). For the ORAC assay, the greatest antioxidant activity was shown by resveratrol, followed by oxyresveratrol, pterostilbene, and pinosylvin, while in the ABTS.+ assay, the highest antioxidant capacity was presented by oxyresveratrol, followed by resveratrol, pinosylvin, and finally pterostilbene. In the FRAP assay, the reducing activity shown by all the stilbenes was below that obtained for trolox. The role of phenolic hydroxyl groups was studied. The technique used should be selected taking into account the objectives and the conditions of the medium.
Open Access
Comunicaciones Orales.-Estudio de la nanoencapsulación molecular del ácido Ruménico con diferentes tipos de ciclodextrinas.
(2020-05-28) García Hernández Gil, Carlos J.; Matencio Durán, Adrián; López Nicolás, José Manuel; García Carmona, Francisco; Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular A
Open Access
Purification and characterization of Terfezia claveryi TcCAT-1, a desert truffle catalase upregulated in mycorrhizal symbiosis
(Public Library of Science, 2019-07-10) Marqués Gálvez, José Eduardo; Morte Gómez, María Asunción; Navarro Ródenas, Alfonso; Pérez Gilabert, Manuela; García Carmona, Francisco; Biología Vegetal; Facultades de la UMU::Facultad de Biología
Terfezia claveryi Chatin is a mycorrhizal fungus that forms ectendomycorrhizal associations with plants of Helianthemum genus. Its appreciated edibility and drought resistance make this fungus a potential alternative crop in arid and semiarid areas of the Mediterranean region. In order to increase the knowledge about the biology of this fungus in terms of mycorrhiza formation and response to drought stress, a catalase from T. claveryi (TcCAT-1) has been purified to apparent homogeneity and biochemically characterized; in addition, the expression pattern of this enzyme during different stages of T. claveryi biological cycle and under drought stress conditions are reported. The results obtained, together with the phylogenetic analysis and homology modeling, indicate that TcCAT-1 is a homotetramer large subunit size monofunctional-heme catalase belonging to Clade 2. The highest expression of this enzyme occurs in mature mycorrhiza, revealing a possible role in mycorrhiza colonization, but it is not upregulated under drought stress. However, the H2O2 content of mycorrhizal plants submitted to drought stress is lower than in well watered treatments, suggesting that mycorrhization improves the plant’s oxidative stress response, although not via TcCAT-1 upregulation.