Martín-Orozco Santiago, María Elena

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Martín-Orozco Santiago, María Elena

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Martín-Orozco Santiago, María Elena

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Universidad de Murcia. Departamento de Bioquímica y Biología Molecular"B" e Inmunología

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Peritoneal macrophage priming in cirrhosis is related to ERK phosphorylation and IL-6 secretion.
(Wiley, 2010-08-19) Ruiz Alcaraz, Antonio José; Martínez-Esparza Alvargonzález, María Concepción; Caño, Rocío; Hernández Caselles, Trinidad; Ricarti, Chiara; Llanos, Lucía; Zapater, Pedro; Martín-Orozco Santiago, María Elena; Pérez Mateo, Miguel; Such, José; García Peñarrubia, María del Pilar; Francés, Rubén; Tapia Abellán, Ana; Bioquímica y Biología Molecular B e Inmunología
Background: Bacterial infections are common complications arising in patients with cirrhosis and ascites. Translocation of bacterial DNA is a dynamic process that is associated with an increased inflammatory response and a poor prognosis in this setting. The aim of this study was to study whether peritoneal macrophages remain in a chronic primed status to allow a rapid response to subsequent events of bacterial translocation. Patients and methods: Peritoneal monocyte-derived macrophages were isolated from 25 patients with cirrhosis and non-infected ascites and compared with donor's blood monocytes. Activation cell-surface markers were screened using flow-cytometry, and the phosphorylation state of ERK 1/2, p38 MAP Kinase, PKB/Akt and transcription factors c-Jun and p65 NFκB were evaluated using Western blot. Synthesis of tumour necrosis factor alpha, interleukin 6 (IL-6) and interleukin-10 (IL-10) at baseline and in response to bacterial stimuli was evaluated using ELISA. Results: A high expression of CD54, CD86 and HLA-DR at baseline was displayed by peritoneal macrophages. Increased phosphorylated levels of ERK1/2, protein kinase B (PKB) and c-Jun, together with IL-6 production, were observed in peritoneal macrophages at baseline compared with donors' blood monocytes. A positive correlation was established between basal IL-6 levels and extracellular signal-regulated kinase (ERK) phosphorylation in peritoneal macrophages from patients with cirrhosis (r=0·9; P=0·005). Addition of lipopolysaccharide induced higher phosphorylation levels of all studied signalling intermediates than synthetic-oligodeoxydinucleotides, but similar end-stage p65 NFκB. Conclusions: A sustained immune response is present in ascitic fluid of cirrhotic patients, even in the temporal absence of bacterial antigens. This would facilitate a fast response, probably controlled by IL-6, against repeated bacterial-DNA translocation or in liver chronic inflammation.
Open Access
Cytokine profiles in cord blood in relation to prenatal traffic-related air pollution: The NELA cohort
(Wiley, 2022-02) García-Serna, Azahara M.; Jiménez-Guerrero, Pedro; Cantero-Cano, Esther; Muñoz-García, María; Molina-Ruano, María Dolores; Rojo-Atenza, Encarna; Hernández Caselles, Trinidad; Martín-Orozco Santiago, María Elena; Morales Bartolomé, Eva; Pérez Fernández, Virginia; García-Marcos Álvarez, Luis Vicente; Ciencias Sociosanitarias
Background: Outdoor air pollution may disturb immune system development. We investigated whether gestational exposure to traffic-related air pollutants (TRAP) is associated with unstimulated cytokine profiles in newborns. Methods: Data come from 235 newborns of the NELA cohort. Innate response-related cytokines (IL-6, IFN-α, IL1-β, and TNF-α), Th1-related (IFN-γ and IL-2), Th2-related (IL-4, IL-5, and IL-13), Th17-related (IL-17 and IL-23), and immunomodulatory cytokine IL-10 were quantified in the supernatant of unstimulated whole umbilical cord blood cells after 7 days of culture using the Luminex technology. Dispersion/chemical transport modeling was used to estimate long-term (whole pregnancy and trimesters) and short-term (15 days before delivery) residential exposures to traffic-related nitrogen dioxide (NO2 ), particulate matter (PM2.5 and PM10 ), and ozone (O3 ). We fitted multivariable logistic regression, Bayesian kernel machine regression (BKMR), and weighted quantile sum (WQS) regression models. Results: NO2 during the whole pregnancy increased the odds of detection of IL-1β (OR per 10 µg/m3 increase = 1.37; 95% CI, 1.02, 1.85) and IL-6 (OR per 10 µg/m3 increase = 1.32; 95% CI 1.00, 1.75). Increased odds of detected concentrations of IL-10 was found in newborns exposed during whole pregnancy to higher levels of NO2 (OR per 10 µg/m3 increase = 1.30; 95% CI 0.99, 1.69), PM10 (OR per 10 µg/m3 increase = 1.49; 95% CI 0.95, 2.33), and PM2.5 (OR per 5 µg/m3 increase = 1.56; 95% CI 0.97, 2.51). Exposure to O3 during the whole pregnancy increased the odds of detected IL-13 (OR per 10 µg/m3 increase = 1.22; 95% CI 1.01, 1.49). WQS model revealed first and third trimesters of gestation as windows of higher susceptibility. Conclusions: Gestational exposure to TRAP may increase detection of pro-inflammatory, Th2-related, and T regulatory cytokines in newborns. These changes might influence immune system responses later in life.
Open Access
Cytokine production by newborns: influence of sex and season of birth
(Natureportfolio, 2022-08-09) García-Serna, Azahara M; Cantero-Cano, Ester; Norte-Muñoz, María; Gil-Buendía, M. Ángeles; Velázquez-Marín, Josefa; Martínez-Torres, Antonia E; Hernández Caselles, Trinidad; Martín-Orozco Santiago, María Elena; Morales Bartolomé, Eva; Pérez Fernández, Virginia; García-Marcos Álvarez, Luis Vicente; Bioquímica y Biología Molecular B e Inmunología
Background: Immune signatures at birth could be associated with clinical outcomes and will improve our understanding of immunity prenatal programming. Methods: Data come from 235 newborns from the cohort study NELA. Production of cytokines was determined using Luminex technology. Associations between cytokine concentrations with sex and season of birth were examined by multivariate regression models. Results: Umbilical cord blood cells produced high levels of inflammatory cytokines, moderate levels of Th1/Th2/Tr-related cytokines, and low levels of Th17 cytokines. Compared to females, male newborn cells secreted higher levels of Th2 (peptidoglycan-stimulated IL-13, odds ratio [OR] = 2.26; 95% CI 1.18, 4.31, p value = 0.013) and Th17 (polyinosinic:polycytidylic acid-stimulated IL-23, OR = 1.82, 95% CI 1.01, 3.27, p value = 0.046) and lower levels of Th1 (olive-stimulated IL-2, OR = 0.56, 95% CI 0.31, 0.99, p value = 0.047) cytokines. Also, children born during warm seasons showed decreased innate cytokine response to peptidoglycan (IL-6, OR = 0.28, 95% CI 0.15, 0.52, p value < 0.001) compared to those born in cold seasons; meanwhile, adaptive immunity cytokines were more frequently secreted by children born during warm seasons in response to allergen extracts (IL-10, OR = 2.11, 95% CI 1.12, 3.96, p value = 0.020; IL-17F, OR = 3.31, 95% CI 1.83, 5.99, p value < 0.001). Conclusion: Newborns showed specific cytokines signatures influenced by sex and season of birth. Impact: There is a limited number of population-based studies on the immune status at birth and the influence of prenatal and perinatal factors on it. Characterization of cytokine signatures at birth related to the prenatal environment could improve our understanding of immunity prenatal programming. Newborns exhibit specific unstimulated and stimulated cytokine signatures influenced by sex and season of birth. Unstimulated and stimulated cytokine signatures in newborns may be associated with the development of related clinical outcomes later in life.
Open Access
Cytokine profiles in cord blood in relation to prenatal traffic-related air pollution: The NELA cohort
(2022-02-18) García-Serna, Azahara M; Jiménez-Guerrero, Pedro; Cantero-Cano, Esther; Muñoz-García, María; Molina-Ruano, María Dolores; Rojo-Atenza, Encarna; Hernández Caselles, Trinidad; Martín-Orozco Santiago, María Elena; Morales Bartolomé, Eva; Pérez Fernández, Virginia; García-Marcos Álvarez, Luis Vicente; Bioquímica y Biología Molecular B e Inmunología
Background: Outdoor air pollution may disturb immune system development. We investigated whether gestational exposure to traffic-related air pollutants (TRAP) is associated with unstimulated cytokine profiles in newborns. Methods: Data come from 235 newborns of the NELA cohort. Innate response-related cytokines (IL-6, IFN-α, IL1-β, and TNF-α), Th1-related (IFN-γ and IL-2), Th2-related (IL-4, IL-5, and IL-13), Th17-related (IL-17 and IL-23), and immunomodulatory cytokine IL-10 were quantified in the supernatant of unstimulated whole umbilical cord blood cells after 7 days of culture using the Luminex technology. Dispersion/chemical transport modeling was used to estimate long-term (whole pregnancy and trimesters) and short-term (15 days before delivery) residential exposures to traffic-related nitrogen dioxide (NO2 ), particulate matter (PM2.5 and PM10 ), and ozone (O3 ). We fitted multivariable logistic regression, Bayesian kernel machine regression (BKMR), and weighted quantile sum (WQS) regression models. Results: NO2 during the whole pregnancy increased the odds of detection of IL-1β (OR per 10 µg/m3 increase = 1.37; 95% CI, 1.02, 1.85) and IL-6 (OR per 10 µg/m3 increase = 1.32; 95% CI 1.00, 1.75). Increased odds of detected concentrations of IL-10 was found in newborns exposed during whole pregnancy to higher levels of NO2 (OR per 10 µg/m3 increase = 1.30; 95% CI 0.99, 1.69), PM10 (OR per 10 µg/m3 increase = 1.49; 95% CI 0.95, 2.33), and PM2.5 (OR per 5 µg/m3 increase = 1.56; 95% CI 0.97, 2.51). Exposure to O3 during the whole pregnancy increased the odds of detected IL-13 (OR per 10 µg/m3 increase = 1.22; 95% CI 1.01, 1.49). WQS model revealed first and third trimesters of gestation as windows of higher susceptibility. Conclusions: Gestational exposure to TRAP may increase detection of pro-inflammatory, Th2-related, and T regulatory cytokines in newborns. These changes might influence immune system responses later in life.
Open Access
Prenatal and Perinatal Environmental Influences Shaping the Neonatal Immune System: A Focus on Asthma and Allergy Origins
(MDPI, 2021-04-09) García-Serna, Azahara M; Hernández Caselles, Trinidad; Martín-Orozco Santiago, María Elena; Morales Bartolomé, Eva; Bioquímica y Biología Molecular B e Inmunología
It is suggested that programming of the immune system starts before birth and is shaped by environmental influences acting during critical windows of susceptibility for human development. Prenatal and perinatal exposure to physiological, biological, physical, or chemical factors can trigger permanent, irreversible changes to the developing immune system, which may be reflected in cord blood of neonates. The aim of this narrative review is to summarize the evidence on the role of the prenatal and perinatal environment, including season of birth, mode of delivery, exposure to common allergens, a farming environment, pet ownership, and exposure to tobacco smoking and pollutants, in shaping the immune cell populations and cytokines at birth in humans. We also discuss how reported disruptions in the immune system at birth might contribute to the development of asthma and related allergic manifestations later in life.
Open Access
Editorial: Wnt Signaling in Immune Cell Regulation During Microbial Infection and Cancer
(Frontiers, 2020-06-05) Blumenthal, Antje; Mc Bride, Jere W; Carson, Dennis A; Sen, Malini; Martín-Orozco Santiago, María Elena; Bioquímica y Biología Molecular B e Inmunología
Open Access
XXVI Congreso Nacional y II Congreso Internacional de SEDEM
(2024-11-28) Abad Valle, J; Abarca, D.; Abdel Moustafa, H.; Acebes, C.; Acosta-Ballester, Y; Affinito, M.; Aguado-Henche, S.; Aguilar Aguilar, G; Aguilar, M.; Alameda Cuesta, A.; Alarcón-Mella, A; Romero Alemán, M.M.; Centeno Cortés, C.; Camacho Morales, J A; Martínez Mora, C.; Trelles, M.; Penas-Lago, C.; Jofré, P.; Blanco Reina, E.; Roque Rojas, F.; Pla-Salas, X.; Recacha Ponce, P; Gomar Sancho, C.; Alberdi Navarro, J; Melo Almiñana, M.; De La Fuente, J.V.; Denizon Arranz, S.; Lafuente Ibáñez De Mendoza, I.; Flores Buils, R; Espíldora Hernández, J.; Borrego, L.; Lafuente Sánchez, J V; Basaras, M.; Moyano, E.; Fernández Jiménez, A.; Rodríguez Vázquez, R.; Chinchilla, A.; Sáenz, N.; Sevillano, E.; Barrios, C.; Ganfornina Álvarez, M.D.; Cabello Salmeron, M.; Arjona Valladares, A.; Aparicio, C.; Miramontes-González, J. P.; Paredes, C. N.; Casaña-Mohedo, J.; Menéndez-González, M.; Geribaldi-Doldán, N.; Villalon, F.J.; Montaldo Lorca, G.A.; Lafuente, I.; Berenguer Vidal, R.; Callau, J.; Lerma Irureta, D.; Riera Claret, C.; Wilke, A.; del Peral Rodríguez, L.J.; Bilbao, J.R.; Martinez Lopez, J; Royo, M.; Rodríguez, F.; Gámez Cabero, M.I.; Castagnetto, J.M.; Vahamaki, A.; Suescun-Elizalde, D.; Lafuente, J V; Gonzales, S.; De la Iglesia Martin, C.; González, A.; Badiola, I.; Sánchez-Gomar, I.; Amelio Garofalo, M D; García-Gargallo, C.; Morales-Gutiérrez, I.; Lagares Gómez-Abascal, A.; Muñoz, K.; Hans Olguín, E R; Salas-Medina, P.; Parra-Ponce, P; Magllón-Botaya, M.R.; Hurtado, O; Ghilotti Rodriguez, J.; Espina, E; Arráez-Aybar, L A.; Guillén García, S.; Granado, C.; Fernández Ros, N.; García Barrios, A.; Maldonado-Bautista, E.; Mangialavori-Rasia, E.; Ríos-Teillier, M; Arbea Moreno, L; Reyes González, M.; Camps Gómez, A.; Suarez Alcázar, M P; Prats, V; Alcolea Cosín, M.T.; López Román, A.; Sáenz Galilea, M.A.; Echeverria Pérez, P.; de Almeida Troncon, L.E.; Montoya Miñana, J.J.; Atutxa, A; Manich, G.; Colom, M.F.; Sainz Gil, M.; Revilla Gómez, N; Hurtado, B.; Biscaia Fernández, J.M.; Munuera, J.L.; Ferrer López, E.; Fonseca, I.; Marín, S.; Gigoux, J.; García García, J.; Cordero, M.; Martín de Llano, J J; Parro Moreno, A.I.; Garcia-Pardina, A.; Ruiz Moral, R.; Alonso Alconada, D.; Sánchez, D.; Talló, M.; Cavero, N.A.; González Soler, E.M.; Cuesta Rubio, N.; Caplliure, J.; Arsis, A.; Pino, C.; Díaz, L.; Muñoz, C.; Herrera, E.; Zapata-Martínez, I.; Ortiz Sanz, C.; Martín Lesende, I; Cid Expósito, M.G.; Cuixart, L.L.; Rubio García, R.; Benito Garzón, L.; Flores, P.; Cisterna, C; Montalván, V.; Salgado, B.; Ruíz-López, J. L.; González Saavedra, I.; Diez Lobato, R.; Vidal-Alaball, J.; Martín Montañez, E.; Iñarrairaegui Bastarrica, M; Ferrer, M.; Apiñaniz Fernandez de Larrinoa, A; Casanoves, E; Sepulveda, R.; Caballo Manuel, C; Guillén-Cañas, V.; Oyanedel, R.; Martin Perez, E.; Lizcano Álvarez, A.; Santiago-Martínez, P.; Piqué, J.; Mirapeix, R.M.; López-Herce Cid, J.; Herrero De La Parte, B.; García Garrido, E.; Gayarre, R.; Carabantes Alarcón, D.; Lara Muñoz, P.; Garcia-Serradilla, M.; Angulo Carrere, M. T.; Modrego Iranzo, D.; Arrarte Esteban, V.; Cano, A.; Rosety Rodríguez, I.; Alfonso, L.; Trampal, A.; Rius-Diaz, F.; Medina, C.; Sánchez-Urtaza, L.; Garcia de Leonardo, C; Fernandez, M; Ortuzar, A; Villena Garrido, M.V.; Casal-Angulo, M C; Mohamed Salem Hameida, I.; Alonso Carrión, L.; SanMartín, M.; López-Brotons, M.; Ciuffreda, M.; Pascual, P.; Ghilotti, J.; Andreu Cervera, A.; Mohedano Moriano, A; Herruzo, I.; Granados Aparici, S; Fernández Barredo, M.; Molina, M.; Gutiérrez Misis, A.; Sánchez Gómez, S.; Brotons de los Reyes, P.; Baquedano-Rodríguez, M.; Tristán Calvo, C.; Almodóvar-Fernández, I.; Jimenez Belio, E.; Fernández-Trillo, J.; de la Peña García, E.; Cariñanos Ayala, S.; Álvarez Vargas, A.P.; Peinado Peinado, R.; Llorente Ortega, M.; Bermejo Fernández-Nieto, J.; Pérez-Urdiales, I.; Molinuevo, B.; Espinar-Herranz, K.; Fernández, A.; Salgueiro, M.; Chevalier, M; Etxebarria, A.; Ortiz-García, F J; Santiago, M.J.; Sánchez Sempere, A.; Jensen, W.; Furtado, J.M.; Yuste, S.; Monasterio Gangoiti, G.; Lorenzo-Álvarez, R.; Gigoux, J; Almeida Troncon, L.E.; Stolle Arranz, A.; Criado Álvarez, J J; Lardiés Uriol, P.; Phillips, C.; Monferrer Garzarán, E; Martínez Pérez, Ó.; Silva Paula, J.; Reguera Puertas, J. C.; Narro, Á; Aramburu Chinchurreta, I.; Granell, E.; de la Torre Perez, J; Martínez García, M.; Gutierrez-Larraya, F.; Cabello Porras, M.R.; Navarro Noguera, A; Cardinal-Fernández, P.; Baliño, P.; Salas Medina, P; Alayola Sansores, A; Valdespina Aguilar, C; Rubio-López, A.; Moyano, E.; Serna Sagarminaga, M.J.; Gonzalez-Soltero, R.; Gea, A.; Pérez-Villalobos, C.; Zarandieta Román, L.; Neria, F; Bruzos-Cidón, C.; Serrat-García, L.; Melendreas Ruiz, R.; Everett, M.; Pla, M.; Herrero Santos, J.I.; Cabedo, H.; Noguera Salvá, R; López de Andrés, A. I.; García-Gómez, S.; León-herrera, S.; Sawczyn, G.; Coronado Vázquez, V; Castellvi Obiols, P.; Valenzuela Cid, D.; Bravo-Llatas, C.; Machuca, I.; Aliaga, J.; Caballero Martinez, F; Portela-Romero, M.; Martínez, S.; Cidad Velasco, P.; Pulido Requero, E.; Ocampos Benedetti, S.; García Lázaro, M.I.; Gea Sanchez, A; Santisteban-Espejo, A.; Garcia Zapirain, B.; Asensio-Gomez, L.; Simón Bratos, I.; Segovia, J.; Luordo, D.; González González, J; Berduzco, N.; Ocampos, S; Méndez-López, F.; Herrero-Alonso, A.; Tórtora, V; Fernández-Mateos, P; Martínez Hernández, D E; Rabanal, Y.; Ochoa de Retana, A.M.; Morales García, J.A.; Tárraga López, P. J.; Romero-Yesa, S.; Pereira, J.L.; Candelmi, D.; Llena Puy, C.; Flores Cuadrado, A; Monge, D; Limón, E.; Cuadrado Corrales, N.; Aschenbrenner-Noriega, I.; De Lama, R.; Castañeda Vozmediano, R.; Santiago Lozano, M.J.; Bilbao Uriarte, E; Salinas Gabiña, I.; Parraga Ramirez, M.J.; Suárez-Alcázar, M.P.; Jimenez, C.G.; Hernandez Pérez, C.; Querencias Martínez, A.; Bermúdez-Barquero, J.; Pérez García, G.N.; Coronado Vázquez, V.; Sunsundegui Seviné, P.; García-Alonso, M.C.; Rey-Aldana, D.; Llussà, J.; Cristóbal-Aguado, S.; Pérez Lambás, F; Mateo, E.; Guillén, V.; Pérez Martínez, M.; Martinez Marcos, A; Soria Aznar, M.; Dupouy, A.; Viñuela Sánchez, A; Coro-Montanet, G.; Miguelez, C.; Hermida, S.; Borrás, F.; Cortés-Fraile, L.; Reyes Serpa, W.; Moreno-Caño, A.; Espinoza-Riffo, M; Pérez Soto, M.I.; Antelo-Pais, P.; Hernández Garrido, M.; Simón-Pérez, C.; Guerrero Orriach, J. L.; Cartanyà, J.; Telleria, A.; Sorrel, M A; Mérida-Velasco, J.R.; Izu Belloso, R.M.; Perez Urdiales, I; Muñoz-Tomás, A.; López Abadía, I.; Valdenebro, S.; Garrido, E.; Arredondo Lamas, J.J.; Rodríguez, X.; Perez Urdiales, I.; Orueta, I.; Trullàs, J-C.; Presa Gutierrez, E.; De la Cruz Conty, M.L.; Martínez-Couselo, J.; Gili, M.; Ortega-Bastidas, J; Martínez Pérez, O.; Fernández-Quintela, A.; Carrillo-Herrero, I.; Flores-Buils, R.; Díez Revuelta, Á.; Cisneros Gimeno, A.I.; Santisteban, L; Piquer, N.; Trampal, A.; Magallón Botaya, R.; Llorens, S.; Rodriguez-Márquez, M. A.; Cinto Pastora, E.; Prieto Nieto, I.; Fernández Linsenbarth, I.; Ros, R.; Mateo Pascual, C.; Fernández-Ponce, C.; Puchol Paseiro, R.; Moreno, M.; Larramona, P.; Puertas, A.; Montalvo Jave, E E; Torrecilla, M.; Zarandona, J.; Fernández Campos, M J; Muriach, M.; Llauger, M.A.; Corbalán-Fernández, A M; Fernández-Aedo, I.; Bakni, M.; Paragis, P.; García López, R.; Pueyo Val, J.; Llena, C.; Tojo-Mañá, W.; Araluce, P.; Delgado Martinez, J.; Tung Chen, Y.; Torres Arrese, M.; Muriach Saurí, M; Lozano, R.M.; Pueyo Villoslada, J; Pastén, D.; Villalón, F.; Hechenleitner-Carvallo, M; Alcaide Ocaña, A.B.; Ruiz, J L; Avedillo Salas, A.; Pérez, J.; Valiente-Ballesteros, C.; Rivero Ruilope, C.; Ferreira, S.; Moreno Cascales, M.; Pérez-Merino, M.; Morales Delgado, N.; Garrido, F.; del Río, J.; Giné Domínguez, E.; Gal, B.; Estefanía, R.; Simoni, J.; Reinoso Lozano, F.; Valdivia, A.; Martínez-Sanz, E.; Ruiz Sauri, A; Espinoza, B.; Riverola Aso, A.P.; García-Santisteban, I.; Lamoglia, M.; Saíz Sánchez, D; Moreno-Gómez-Toledano, R.; Alvarez, MN; Martinez Jabares, S.; Rodríguez Boto, G.; Ortiz, M. A.; Urrutia, S.; Bernal Ruiz, M.L.; García-Nieto, M.; Peralta-Camposano, J; Martínez Ricós, J.; Fernández Arias, C.; García, E.; Pino-Valenzuela, D.; Gravina, L.; Babarro, I.; Martínez-Pecharromán, M.M.; Tutor, A.S.; Campos Lucas, F.J.; Folguera Ferrairo, S.; Vallverdú-Cartié, H.; Urtubia-Fernández, J.; Penas, C.; Rodríguez Cruz, L.M.; Pons, M; Zamorano León, J. J.; Llanes Pelegrín, R.J.; Blanco Alfonso, A.; Sánchez-Gallegos, P.; Alcaine, C.; Quiroz, A.; Virto-Ruíz, L.; Rubio-Navas, A.; Arteaga-San Martín, R; Pujol, R.; Ortuzar, A.; Antonio Vasquez, B E; Folch Ayora, A.; Hijano, F.; González López, E.; García Uriaguereca, M E; Toledo Atucha, E.; Romo Barrientos, C; Moreno Cerda, M.I.; Ballesteros, I.; Álvarez-Montero, S; Rubio-Perez, I.; Gutierrez-Aguirregabiria, A.; Bández-Ruiz, M.; Paragis, P.; Caballero, L.M.; Ruiz de Azua García, S.; Ruiz de Azúa, S.; Pérez-Villalobos, C; Veronese Rodrigues, M.L.; Cinza-Sanjurjo, S.; Gutierrez Misis, A.; Prats-Martínez, V.; Morenilla-Palao, C.; Alvarez, A.; Hancco, D.; Raurell, M.; Casanoves-Laparra, E.; Arrimadas, S; Bartolomé, B.; de la Calle, C; Gómez, L.; Virumbrales Cancio, M.; Vicente Romero, J.; Posadas, A.; Ruiz de Gauna, P; Azkue, J. J.; Martínez Reguera, V.; Collado Boira, E; Sepulveda, R; Blanco Canseco, J.M.; Rodríguez, T.; Zuluaga-Arias, P; Salobrar-García, E; Carrasco Yubero, J; Rodríguez Rivero, L.E.; Perez, I.; Gómez Trullén, E.M.P.; López de Ayala, J.M.; Javaloy-Ballestero, S.; Guerra, L.; Ubeda Bañon, I; Sáez, F.J.; Pallas, M.; Castaños, A.; Alba-Linero, C.; Salvador López, R.; Monforte Martínez, C.; Castelao, O.; Astillero López, V; Crespo-Sánchez, S.; San Sebastian, K.; Sanabria-Cabrera, J.; Puras, G.; Basco, L.; Esteban, S.; Latorre, A.; Pacheco Jimenez, L.M.; Caballero, F; Linares Aranda, M.; Rojo Ruiz, J.; Diaz, J.A.; Cardozo González, G; Cendrós, V.; Ferreira Schettini, S; Idoate, A.; Zurita, R.; Ricouz-Moya, A.; Sánches Ramírez, O; Casanova Turnez, P; Morenilla Palao, C.; Fairén, E.; Soto Castellón, B.; Collado, L; Ruíz-López, J.L.; Bertomeu González, V.; Santos Amaya, I.; Ruiz Montesinos, I.; Quiroga Vila, J.; Ibarretxe, G.; Meana González, C.; Lucena Ramírez, F.; Lajara Blesa, J.J.; van-Zon-Herrera, P.; Sandoval Araneda, N.; Pinilla-Roa, A.E.; Díez, N.; Sanz Miguel, C.; Olaizola, J. I.; Dios Parada, N.; Casado Herráez, C.; Fernandez-Jimenez, N.; Sanz, E.J.; Sarmiento, B.K.; Rodríguez-Díez, C.; Cervera Barba, E.; García Ruiz de Gordejuela, A.; Piccinato, C.E.; Aleo, E.; Arias-Díaz, J; Baliño Remiro, P; Fortún Landecho Acha, M.; De Léón, N; Echevarría, E.; Gea, A.; López-Ortiz, L.; Betancor, L; Sasal Pérez, S.; Díaz de Castellvi, S.; Ozamiz, N.; Garcia-Alvarez, M.; Tórtora Peduto, V; Fanlo Villacampa, A.J.; Sánchez-Calavera, M.A.; Alegre, M.; Smits, N; Sánchez Gómez, S; López-Herce, J.; Morera-Herreras, T.; Meneses, J.; Arce-Vázquez, V.; Vitali Magasich, G.; Serra Ribeiro, M.; Ramos Uriarte, A.; Gallego, L.; Sojo-Moyano, N; Pérez-Caballer, A.; Delgado, J.; Garrido-Aguilar, E.; Solana García, M.J.; Barbancho-Fernández, M.Á.; Ruiz Palomino, E; Eraso, E.; Guadalajara Labajo, H.; Aveiro Colares, M.F.; Borque Roda, I.; Caparrós Cayuela, E.; González Cortés, R.; Flores-Sáenz, M.; Álvarez Vázquez, M. P.; Hernández, C.; Mencía Bartolomé, S.; Ciriza, J.; Solanas, E.M.; Forner, L.; Piccinato, C.E.; Guillén Cañas, V; García-Carmona, R.; Segurado Calvo, A.; Blasco Serra, A.; Tomás Caballero, M.; Sawczyn, G.; Melo, M.; Paredes, S; Muñoz, J.R.; Cantillo Lucuara, M; Bastías-Vega, N; Rojí Buqueras, R.; González Perilli, L I; Tomasina Origuela, F; Puiggrós-Binefa, A.; Folguera, S.; Hawes, G; Tormo-Calandín, C; Castelo Jurado, M.; Sánchez Romero, D.; Garcia de Pereda-Notario, C.M.; Casanova Delgado, M.; Alegría, E.; de la Peña Fernández, L.; Apellaniz, I.; Goitia, L.; Caballero-Sánchez, S.; Llorente Ortega, M; Muñoa, M.; Estebanez, R.; Barrio, E.; Raigón Ponferrada, A.; Lamus Molina, J.F.; Guillén Cañas, V.; Soto Araya, M.; Condori, F.A.; Ulibarrena, A.; Solas Zubiaurre, M.; Arrue, M.; Piñuñuri, R.; Cervera Ferri, A.; Sabatella Larramendi, L; Javier, R.M.; Unanue-Arza, S.; Rodríguez Gómez, P; Goni Rasia, G.; Llamazares Medrano, J M; Unda, F.J.; Durán Alonso, B.; Schilling-Norman, M; Denizón, S; Bonilla Hernández, L. M.; Filipeli Chiarelli, C; Cambra Badii, I.; Hilario, E.; Cosano, P.; Martínez, A.; Arteta, B.; García Sánchez, O.; Castón, J. J.; Martín, I.; Lanuza Giménez, F.J.; Torres, R.; Faundez Inostroza, K.M.; Varilla Ezkurra, I.; Torre-Cisneros, J.; Carhuancho, J.R.; Recacha-Ponce, P.; Portillo, M.P.; Escamilla Guinea, S.; Lucena-González, M.I.; Vásquez-López, J. A.; Balaguer Santamaría, A.; Idoyaga, M; Martínez Regada, X.; Vázquez Calatayud, M; García Alonso, I.; Soria, M.; Forner Navarro, L.; Contreras, C; De Jesús, P.; López Pingarrón, L.; Fernández-García, J.M.; Moreno Planas, J. M.; Carretero, C.; Guerra Sánchez, M.; Durántez Fernández, C; Monforte-Martínez, C.; Martínez Garcia, M.; Ustarro Cano, M L; Vivanco, L.; Urbano Villaescusa, J.; De Moura Reis, L.; Romero-Cardona, A.; Ricouz-Moya, A; García del Barrio, L; Mengual, E.; Vivanco, L.; Casas Requena, J.; Arana, J; Vuyk, I; González Tejerina, B; Torrejón Cabanillas, T.; Gonzáles, M.H.; Fernández Aragón, C.; Álvarez Cal, M N; Medina Caliz, I.; Gargiulo, P Á; Paradas-Lara, I.; del Río-Salas, A.; Oleaga, J.; Monge-Martín, D; Vicente-García, C.; Beato, L.; Vergara, R.; Álvarez Gómez, E; Sáenz-del-Burgo, L.; Rosety-Rodriguez, I.; Gonzalez, L; Bellido Estevez, I.; Diez de Ulzurrun Muruzabal, M.; Canduela Pérez, M.J.; Bellido, A.; Jiménez García, R.; Barroso González, A.; Iglesias, J.J.; García-Garrido, E.; Bulnes, S; Movellán Luis, M; Pino-Valenzuela, D.; Folch Ayora, A; Gonzalez-Caminal, G.; Pérez-Merino, M.; Arteaga González, N I; Yáñez-Freire, S.; Alegre Climent, M; Whyte Orozco, J.; Burgos-Alonso, N.; Sánchez-Vera, I.; Jimeno Bulnes, N.; Cervera, E; Fresco, R.E.; de La Cueva, L.; Cervilla, A.B.; Machuca, I.; Cazorla Morallón, D.; Lorenzo Ruiz, I.; Antón Rodríguez, C.; Corral-Gudino, L.; Ramos-Rincon, J.M.; Gómez Luque, A.; Jiménez Sierra, L.; Puisac Uriol, B.; Carmenate, M; Beño Ruiz de la Sierra, R.M.; Blanco, J M; Bengoetxea, H; Cortegano Jimeno, I.; Serpa Pérez, P.C.; Esnaola Guisasola, P.M.; González Gil, M.T.; Ferrer Gomez, L.; Espárrago Bajo, T.; Milton, I.; Echarte Alonso, L. E.; Mencía, S.; Cabello, D.; Fernández Trillo, J.; Rojo Díaz, S.; Madrigal Verdú, P.; Rodríguez Rivero, L E; De Nova Muñoz, J. L.; Chico, B.; Conde-Sabarís, P.; Charneco, G.; Alonso-Varona, A.; Barrio-Asensio, M.C.; García del Barrio, L.; Izquierdo-García, C.; García Fernández, E.; Murillo-González, J.; García De La Fuente, A.M.; Muñoz Izquierdo, D.; Cuesta Sancho, S.; García, A.M.; Sabatella, L.; Mínguez-Rey, M F; Ruiz de Gauna, P.; Flores, P.; Farias Barreto Silva, R.; Espasandín-Duarte, I.; Bueno Martínez, E.; Castillo, H.; González, R.; Lou, J. M.; Gosálvez Vega, A.; Peralta, C.; González-Pinto, A.; Pastén-Araya, D.; Gutierrez, S.; Sánchez-Gomar, I.; Murueta-Goyena, A; Sanchez Fernandez, J.J.; Gojenola, K; Garcia-Estañ, J.; García Fernandez, J.; Corral Vielba, P.; Durán, I.; Jofré, P; Jofré, P.; Prats-Martínez, V.; Jiménez-Rojo, L.; Pombero, A.; Arambarri, J.; Serna Sagarminaga, M J; Pereira, J.L.; Baños, J.E.; Hoyos Cillero, I; Fernández-Villacañas Marín, Miguel Ángel; Guerrero Sánchez, Yolanda; García Bernal, David; Hernández Caselles, Trinidad; Martín-Orozco Santiago, María Elena; Noguera Velasco, José Antonio; Pelegrín Vivancos, Pablo; Roca Soler, Francisco José; Rubio Pedraza, Gonzalo; Valdor Alonso, Rut; Gascón Cánovas, Juan José
Comunicaciones aceptadas en el XXVI Congreso de la Sociedad Española de Educación Médica, celebrado en Bilbao del 28 al 30 de noviembre de 2024.
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Air pollution from traffic during pregnancy impairs newborn's cord blood immune cells: The NELA cohort
(ELSEVIER, 2021) García-Serna, Azahara M; Jiménez-Guerrero, Pedro; Cantero-Cano, Esther; Muñoz-García, María; Ballesteros-Meseguer, Carmen; Pérez de Los Cobos, Irene; NELA Study group; Hernández Caselles, Trinidad; Martín-Orozco Santiago, María Elena; Morales Bartolomé, Eva; Pérez Fernández, Virginia; García-Marcos Álvarez, Luis Vicente; Bioquímica y Biología Molecular B e Inmunología
Background: Hazards of traffic-related air pollution (TRAP) on the developing immune system are poorly understood. We sought to investigate the effects of prenatal exposure to TRAP on cord blood immune cell distributions; and to identify gestational windows of susceptibility. Methods: In-depth immunophenotyping of cord blood leukocyte and lymphocyte subsets was performed by flow cytometry in 190 newborns embedded in the Nutrition in Early Life and Asthma (NELA) birth cohort (2015-2018). Long-term (whole pregnancy and trimesters) and short-term (15-days before delivery) residential exposures to traffic-related nitrogen dioxide (NO2), particulate matter (PM2.5 and PM10), and ozone (O3) were estimated using dispersion/chemical transport modelling. Associations between TRAP concentrations and cord blood immune cell counts were assessed using multivariate Poisson regression models. Results: Mean number of natural killer (NK) cells decreased 15% in relation to higher NO2 concentrations (≥36.4 μg/m3) during whole pregnancy (incidence relative risk (IRR), 0.85; 95% CI, 0.72, 0.99), with stronger associations in the first trimester. Higher PM2.5 concentrations (≥13.3 μg/m3) during whole pregnancy associated with a reduced mean number of cytotoxic T cells (IRR, 0.88; 95% CI, 0.78, 0.99). Newborns exposed to higher PM10 (≥23.6 μg/m3) and PM2.5 concentrations during the first and third trimester showed greater mean number of helper T type 1 (Th1) cells (P < 0.05). Decreased number of regulatory T (Treg) cells was associated with greater short-term NO2 (IRR, 0.90; 95% CI, 0.80, 1.01) and PM10 (IRR, 0.88; 95% CI, 0.77, 0.99) concentrations. Conclusions: Prenatal exposure to TRAP, particularly in early and late gestation, impairs fetal immune system development through disturbances in cord blood leukocyte and lymphocyte distributions.
Open Access
WNT Signaling in Tumors: The Way to Evade Drugs and Immunity
(2019) Sánchez-Fernández, Ana; Ortíz-Parra. Irene; Ayala-San Nicolás, María; Martín-Orozco Santiago, María Elena; Bioquímica y Biología Molecular B e Inmunología
Open Access
WNT Signaling in Tumors: The Way to Evade Drugs and Immunity
(Frontiers, 2019-12-20) Sánchez-Fernández, Ana; Ortíz-Parra, Irene; Ayala San Nicolás, María; Martín-Orozco Santiago, María Elena; Bioquímica y Biología Molecular B e Inmunología
WNT/β-catenin signaling is involved in many physiological processes. Its implication in embryonic development, cell migration, and polarization has been shown. Nevertheless, alterations in this signaling have also been related with pathological events such as sustaining and proliferating the cancer stem cell (CSC) subset present in the tumor bulk. Related with this, WNT signaling has been associated with the maintenance, expansion, and epithelial-mesenchymal transition of stem cells, and furthermore with two distinctive features of this tumor population: therapeutic resistance (MDR, multidrug resistance) and immune escape. These mechanisms are developed and maintained by WNT activation through the transcriptional control of the genes involved in such processes. This review focuses on the description of the best known WNT pathways and the molecules involved in them. Special attention is given to the WNT cascade proteins deregulated in tumors, which have a decisive role in tumor survival. Some of these proteins function as extrusion pumps that, in the course of chemotherapy, expel the drugs from the cells; others help the tumoral cells hide from the immune effector mechanisms. Among the WNT targets involved in drug resistance, the drug extrusion pump MDR-1 (P-GP, ABCB1) and the cell adhesion molecules from the CD44 family are highlighted. The chemokine CCL4 and the immune checkpoint proteins CD47 and PD-L1 are included in the list of WNT target molecules with a role in immunity escape. This pathway should be a main target in cancer therapy as WNT signaling activation is essential for tumor progression and survival, even in the presence of the anti-tumoral immune response and/or antineoplastic drugs. The appropriate design and combination of anti-tumoral strategies, based on the modulation of WNT mediators and/or protein targets, could negatively affect the growth of tumoral cells, improving the efficacy of these types of therapies.