Digitum Colección:
http://hdl.handle.net/10201/74541
2024-03-28T21:58:44ZCharacterization of rat testicular teratoma and
its derived cell lines, with particular reference
to possible mesenchymal differentiations
http://hdl.handle.net/10201/74975
Título: Characterization of rat testicular teratoma and
its derived cell lines, with particular reference
to possible mesenchymal differentiations
Autor/es principal/es: Yamate, Jyoji; Gamou, Katsuhiro; Izawa-Ogata, Keiko; Kotera, Takashi; Izawa, Takeshi; Takenaka, Shigeo; Sawamoto, Osamu; Kuwamura, Mitsuru
Resumen: The original tumor, 4 cm in diameter, was
found in the left testis of a 2-month old SD rat. The
tumor consisted of well-differentiated, mature tissues
such as bone, cartilage, adipose tissue, smooth and
skeletal muscles, skin, hair, glands (salivary, sebaceous,
apocrine and pancreatic exocrine glands) and trachea, as
well as nerve tissues. The tumor was diagnosed as a
mature type of teratoma, a rare in rat testis. Cloned cell
lines (named TSD-B4S and TSD-F9R) were established
from the tumor; cellular properties of these cell lines
were similar to each other; basically, their cultured cells
exhibited vimentin-positive mesenchymal nature with
occasional cells reacting to α-smooth muscle actin, glial
fibrillary acidic protein and CD163 (a macrophage
marker). The cell lines showed tumorigenicity when
inoculated into nude mice, being composed of immature
mesenchymal cells arranged mainly in a sheet. In TSDB4S cells treated with differentiation factors, we
demonstrated mesenchymal differentiations towards
adipogenic, osteogenic and myofibrogenic cells. The cell
line (TSD-B4S) would become a useful tool for studies
on stem cell differentiation, because the teratoma arises
from primordial germ cells like embryonic stem cells.2014-01-01T00:00:00ZAberrant expressions of delta-protocadherins
in the brain of Npc1 mutant mice
http://hdl.handle.net/10201/74953
Título: Aberrant expressions of delta-protocadherins
in the brain of Npc1 mutant mice
Autor/es principal/es: Yan, Xin; Lukas, Jan; Lin, Juntang; Ernst, Mathias; Koczan, Dirk; Witt, Martin; Fuellen, Georg; Wree, Andreas; Rolfs, Arndt; Luo, Jiankai
Resumen: Niemann-Pick type C1 (NPC1) disease is an
autosomal recessive disorder characterized by
dysmyelination and neurodegeneration, which can result
in the death of patients in early childhood in some cases.
Members of the delta-protocadherins (Pcdhs) play
important roles in neurogenesis and brain development.
In this study, we compared expression profiles of Pcdhs
in the brain between wild-type and Npc1 mutant mice
from postnatal day (P) 9 onwards by in situ
hybridization. Our data show that laminar distribution of
some Pcdhs in the cerebral cortex of Npc1 mutated mice
is different from that of wild-type mice. Furthermore,
expressions of Pcdhs by oligodendrocytes in the corpus
callosum and by Purkinje cells and granular cells in the
cerebellum are strongly decreased in Npc1 mutated mice
at later stages. Taken together, our data suggest that
aberrant expression of Pcdhs is a pathological process
accompanied by neurodegeneration in Npc1 mutant
mice.2014-01-01T00:00:00ZAmelioration of hypercholesterolemiainduced hepatic changes with red
grape juice: A histopathological study
http://hdl.handle.net/10201/74952
Título: Amelioration of hypercholesterolemiainduced hepatic changes with red
grape juice: A histopathological study
Autor/es principal/es: Al-Ahmadi, Ahlam Abdulaziz; Ali, Soad Shaker; Ayuob, Nasra Naeim; Al Ansary, Abeer Khaled
Resumen: Objectives: Hypercholesterolemia was
confirmed as a risk factor for hepatic fibrosis, as well
atherosclerosis and coronary heart disease. This
biochemical and histoplathological study was conducted
to investigate the possible protective effect of red grape
against hepatic injury induced by a high-cholesterol diet
(HCD).
Material and methods: Thirty male Wister rats were
randomly divided into three groups (n=10): the control
received saline, the induction group was fed HCD, and
the treated group was fed a HCD and 0.4 ml of 100% red
grape juice (RGJ) for 13 weeks. After the animals were
sacrificed, liver tissue samples were taken to be
processed for light and electron microscopy
examination.
Results: The administration of the RGJ and HCD
significantly decreased the animals’ blood glucose,
insulin, cholesterol, triglycerides, Low Density
Lipoprotein levels and increased their High Density
Lipoprotein level compared to the rats fed the HCD
alone. It also decreased the periportal (macro- and
microvesicular) steatosis, fibrosis, lymphocytic
infiltration and blood sinusoidal congestion that were
observed in HCD-fed rats alone. The RGJ reduced the
number of activated myofibrobasts. This was confirmed
by a reduction in the expression of alpha smooth muscle
actin and desmin. The RGJ increased, although not
significantly, the expression of endothelial Nitric Oxide
Synthetase.
Conclusion: The administration of RGJ succeeded in
alleviating the biochemical and, to some extent, the
histopathological changes induced by the high
cholesterol diet. Consumption of fresh RGJ or its
pharmaceutical preparations is advised especially for
those who are used to eat a high fat diet.2014-01-01T00:00:00ZHsp27 and its expression pattern
in diffusely infiltrating astrocytomas
http://hdl.handle.net/10201/74974
Título: Hsp27 and its expression pattern
in diffusely infiltrating astrocytomas
Autor/es principal/es: Mäkelä, Katri S.; Haapasalo, Joonas A.; Ilvesaro, Joanna M.; Parkkila, Seppo; Paavonen, Timo; Haapasalo, Hannu K.
Resumen: Aims: Heat shock protein 27 (Hsp27) is
induced by cell stress conditions. In the presence of
oxidative stress it functions as an antioxidant. To study
the putative expression patterns and clinical significance
of Hsp27, we assessed the associations between Hsp27,
R132H mutation of Isocitrate dehydrogenase1 (IDH1-
R132H), Hypoxia-inducible factor subunit alpha (HIF-1
alpha), Carbonic anhydrase IX (CA IX), and patient
prognosis in astrocytic gliomas.
Methods: Tissue micro-array samples of 295 grade
II-IV astrocytomas were stained immunohistochemically
for Hsp27, IDH1-R132H, HIF-1 alpha, and CA IX. We
tested their relationship with clinicopathological features
and patient survival.
Results: There was a significant correlation between
Hsp27 expression and increasing WHO grade (p<0.001).
Hsp27 expression correlated significantly with IDH1
mutation when studied within the entire cohort
(p<0.001) as well as separately in WHO grade II and III
tumors (p=0.006 and 0.002, respectively). IDH1
mutation and HIF-1 alpha positive staining were
detected simultaneously (p<0.001). In IDH1 mutated
tumors, positive HIF-1 alpha staining correlated with CA
IX expression (p=0.027), whereas no such correlation
was found in IDH1 non-mutated tumors. IDH1 mutation
was associated with a low cell proliferation index
(p=0.001) and HIF-1 alpha with increasing proliferation
(p=0.003). Hsp27 expression was associated with a
shorter rate of patient survival in univariate survival
analysis (p=0.001). In multivariate survival analysis,
patient age, IDH1 mutation and HIF-1 alpha appeared as
independent prognostic factors (p<0.000, <0.000 and
0.011 respectively)
Conclusions: Hsp27 expression is associated with
increasing WHO grade and patient prognosis in
astrocytic gliomas. The results suggest that IDH1
mutation may have an effect on the expression pathways
of Hsp27 and CA IX.2014-01-01T00:00:00Z