Digitum Colección:
http://hdl.handle.net/10201/29521
2024-03-28T20:21:56ZEvaluation of microvascular density in tumors, pro and contra
http://hdl.handle.net/10201/29811
Título: Evaluation of microvascular density in tumors, pro and contra
Autor/es principal/es: Nico, Beatrice; Benagiano, Vicenzo; Mangieri, Doménica; Maruotti, Nicola; Vacca, Angelo; Ribatti, Doménico
Resumen: Microvascular density (MVD) counting
protocols have become the morphological gold standard
to assess the neovasculature in human tumors. This
method requires the use of specific markers to vascular
endothelium and of immunohistochemical procedures to
visualize microvessels. MVD determined in primary
tumors is significantly associated with metastasis and
prognosis in several tumors and is most predictive in
those tumors that induce significant angiogenesis,
namely carcinomas of breast and prostate, and
haematological malignancies.
There is such a wide range of antibodies and
suppliers, antigen retrieval methods, designation of high
and low vessel count groups, patient study groups and
data interpretation, that it is exceedingly difficult to
compare results.2008-01-01T00:00:00ZThe Notch pathway, hair graying and pigment cell homeostasis
http://hdl.handle.net/10201/29810
Título: The Notch pathway, hair graying and pigment cell homeostasis
Autor/es principal/es: Schouwey, Karine; Beermann, Friedrich
Resumen: The Notch signaling pathway is an essential
cell-cell interaction mechanism, which regulates
processes such as cell proliferation, cell fate decisions,
differentiation or stem cell maintenance. Pigmentation in
mammals is provided by melanocytes, which are derived
from the neural crest, and by the retinal pigment
epithelium (RPE), which is part of the optic cup and
hence orginates from neuroectoderm. The importance of
functional Notch signaling in melanocytes has been
unveiled recently. Here, the pathway is essential for the
maintenance of proper hair pigmentation. Deletion of
Notch1 and Notch2 or RBP-Jk in the melanocyte lineage
resulted in a gene dosage-dependent precocious hair
graying, due to the elimination of melanoblasts and
melanocyte stem cells. Expression data support the idea
that Notch signaling might equally be involved in
development of the RPE. Furthermore, recent analyses
indicate a possible role of Notch signaling in the
development of melanoma. In this review, we address
the essential role of Notch signaling in the regeneration
of the melanocyte population during hair follicle cycles,
and discuss data supporting the implication of this
signaling pathway in RPE development and melanoma.2008-01-01T00:00:00ZCellular localization of insulin-like growth factor-II protein in the sea bass -Dicentrarchus labrax- from hatching to adult
http://hdl.handle.net/10201/29809
Título: Cellular localization of insulin-like growth factor-II protein in the sea bass -Dicentrarchus labrax- from hatching to adult
Autor/es principal/es: Radaelli, G.; Poltronieri, C.; Bertotto, D.; Funkenstein, B.; Simontacchi, C.
Resumen: The cellular localization of IGF-II protein
was investigated during larval and postlarval
developmental stages of sea bass (Dicentrarchus labrax)
by immunohistochemistry using antisera raised against
Sparus aurata IGF-II. At hatching, IGF-II
immunoreactivity was already present in the skin,
developing intestine and skeletal muscle. During larval
life IGF-II protein was also observed in heart
musculature, in kidney and gill epithelia as well as in
liver. In fry skeletal muscle a moderate IGF-II
immunostaining was detected in red fibres, whereas
white muscle fibres exhibited a faint immunoreactivity.
In adults, a marked IGF-II immunostaining was
observed in red muscle fibres. A moderate
immunoreactivity was also present in white fibres as
well as in heart striated myocardial fibres. These results
are in agreement with previous findings on the spatial
localization of IGF-II and IGF type 1 receptor in S.
aurata and Umbrina cirrosa, confirming the role of IGF
system during development and growth of fish.2008-01-01T00:00:00ZExpression of matrix metalloproteinases MMP-2 and MMP-9 in gastric cancer and their relation to claudin-4 expression
http://hdl.handle.net/10201/29808
Título: Expression of matrix metalloproteinases MMP-2 and MMP-9 in gastric cancer and their relation to claudin-4 expression
Autor/es principal/es: Lee, Li-Yu; Wu, Chi-Ming; Wang, Chee-Chan; Yu, Jau-Song; Liang, Ying; Huang, Kuo-Hao; Lo, Chih-Hong; Hwang, Tsann-Long
Resumen: Matrix metalloproteinases (MMPs) MMP-2
and MMP-9 can degrade type IV collagen of
extracellular matrix and basal membranes. Claudin-4 is a
member of a large family of transmembrane proteins,
claudins, essential in the formation and maintenance of
tight junctions. Claudin-4 has been shown to activate
MMP-2, indicating that claudin-mediated increased
cancer cell invasion might be mediated through the
activation of MMP proteins. To explore the roles of
MMP-2, MMP-9 and claudin-4 in gastric cancer, we
selected 88 cases and then analyzed the expression of
these proteins using immunohistochemistry. We found
that all of MMP-2, MMP-9 and claudin-4 expressions
were significantly higher in intestinal-type than in
diffuse-type gastric cancer. On further analysis, testing
the relationship between MMP-2 and MMP-9 expression
with claudin-4 expression, claudin-4 expression was
significantly associated with MMP-9 expression, but not
with MMP-2 expression. The results showed that MMP-
2, MMP-9 and claudin-4 expression may be phenotypic
features, distinguishing intestinal-type and diffuse-type
gastric cancer. Possibly, claudin-4 played a role in
determining MMP-9 activity which favored intestinaltype
gastric cancer to distal metastasis.2008-01-01T00:00:00Z