Digitum Colección:http://hdl.handle.net/10201/543042024-03-28T16:00:18Z2024-03-28T16:00:18ZExpression of matricellular proteins in human uterine leiomyomas and normal myometriumBogusiewicz, MichalSemczuk, AndrzejJuszczak, MalgorzataLangner, EwaWalczak, KatarzynaRzeski, WojciechTomaszewski, JacekRechberger, Tomaszhttp://hdl.handle.net/10201/544682020-02-26T01:22:54Z2012-01-01T00:00:00ZTítulo: Expression of matricellular proteins in human uterine leiomyomas and normal myometrium
Autor/es principal/es: Bogusiewicz, Michal; Semczuk, Andrzej; Juszczak, Malgorzata; Langner, Ewa; Walczak, Katarzyna; Rzeski, Wojciech; Tomaszewski, Jacek; Rechberger, Tomasz
Resumen: Growth of human leiomyomas can probably be initiated as a response to injury, in a way similar to the development of keloids. Among many bioactive molecules, which are implicated in tissue repair, a pivotal role is attributed to matricellular proteins. The aim of the current study was to evaluate the immunohistochemical expression of tenascin-C (TNC), thrombospondin-1 (TSP-1), SPARC/osteonectin and tenascin-X (TNX) in human uterine leiomyomas and normal myometrium. Immunostaining was performed on 33 pairs of paraffin-fixed sections and 9 cell-lines derived from uterine leiomyomas and normal myometrium. Fifteen (45.5%) leiomyomas investigated were positive for TNC, whereas all normal myometrial samples were immunonegative (χ2=19.41; p<0.001). Immunostaining for TSP-1 was observed in 20 (60.6%) uterine fibroids and in 12 (36.4%) control samples (χ2=3.88; p<0.05). The expression of SPARC/osteonectin protein was more frequently found in leiomyomas than in normal myometrium, but this difference was not significant. Apart from one fibroid culture and one myometrial culture, all the others revealed strong TNC immunostaining. Expression of TSP-1 and SPARC/osteonectin was weak to moderate in all established cell-lines. None of the tissues or cell lines investigated showed positive staining for TNX. In conclusion, TSP-1 and TNC are likely to play important roles in the pathogenesis of uterine leiomyomas, presumably affecting cell proliferation and/or extracellular matrix deposition.2012-01-01T00:00:00ZClinicopathologic features of molecular subtypes of triple negative breast cancer based on immunohistochemical markersChoi, JunjeongJung, Woo-HeeKoo, Ja Seunghttp://hdl.handle.net/10201/544672020-02-26T01:22:51Z2012-01-01T00:00:00ZTítulo: Clinicopathologic features of molecular subtypes of triple negative breast cancer based on immunohistochemical markers
Autor/es principal/es: Choi, Junjeong; Jung, Woo-Hee; Koo, Ja Seung
Resumen: This study was performed to identify molecular subtypes of triple negative breast carcinoma (TNBC) based on immunohistochemical markers. We prepared a tissue microarray from TNBC specimens of 122 patients and performed immunohistochemical staining for cytokeratin (CK) 5/6, epidermal growth factor receptor (EGFR), claudin 3, claudin 4, claudin 7, E-cadherin, androgen receptor (AR), and gammma-glutamyltransferase (GGT1). Based on immunoreactivity, tumors were classified into basal-like (CK5/6 positive and/or EGFR positive), molecular apocrine (AR positive and/or GGT1 positive), claudin low (claudin 3, claudin 4, claudin 7 negative and/or E-cadherin negative), mixed (tumors belonging to two or more subtypes), and null (tumors not matching any other subtypes). The TNBC specimens of 122 patients included 27 basal-like (22.1%), 28 claudin low (23.0%), 12 molecular apocrine (9.8%), 23 mixed (18.9%) and 32 null (26.2%) subtype tumors. The molecular apocrine subtype showed the highest percentage of apocrine differentiation and the lowest Ki-67 labeling index (p<0.001 and p=0.040, respectively). In univariate analysis, tumor cell discohesiveness was related with shorter disease free survival (DFS) and overall survival (OS) (p=0.005, and 0.002, respectively). In multivariate analysis, tumor cell discohesiveness was related with shorter OS and CK5/6 positivity (p=0.018), and claudin 7 positivity (p=0.019) was related with shorter DFS. In conclusion, using immunohistochemical staining for CK5/6, EGFR, claudin 3, claudin 4, claudin 7, E-cadherin, AR, and GGT1, we categorized TNBC into a basal-like subtype, a claudin low subtype, a molecular apocrine subtype, a mixed subtype showing characteristics of two different subtypes, and a null subtype not belonging to any of the subtypes identified.2012-01-01T00:00:00ZExperimental diabetes modulates collagen remodelling of joints in ratsAtayde, Sandra A.Yoshinari, Natalino H.Nascimento, Dafne P.Catanozi, SérgioAndrade, Priscila C.Velosa, Ana Paula P.R. Parra, EdwinPassarelli, MarisaNakandakare, Edna R.Capelozzi, Vera L.Teodoro, Walcy R.http://hdl.handle.net/10201/544662020-02-26T01:22:49Z2012-01-01T00:00:00ZTítulo: Experimental diabetes modulates collagen remodelling of joints in rats
Autor/es principal/es: Atayde, Sandra A.; Yoshinari, Natalino H.; Nascimento, Dafne P.; Catanozi, Sérgio; Andrade, Priscila C.; Velosa, Ana Paula P.; R. Parra, Edwin; Passarelli, Marisa; Nakandakare, Edna R.; Capelozzi, Vera L.; Teodoro, Walcy R.
Resumen: The aim of this study was to evaluate extracellular matrix components in articular cartilage, ligaments and synovia in an experimental model of diabetes. Young Wistar rats were divided into a streptozotocin-induced (STZ; 35 mg/kg) diabetic group (DG; n=15) and a control group (CG; n=15). Weight, blood glucose and plasma anti-carboxymethyllysine were measured 70 days after STZ infusions. Knee joints, patellar ligaments, and lateral and medial collateral ligaments were isolated and stained with hematoxylin-eosin and Picrosirius. The total collagen content was determined by morphometry. Immunofluorescence was employed to evaluate types I, III, and V collagen in ligaments and synovial tissues and types II and XI collagen in cartilage. Results: Higher blood glucose levels and plasma anti-carboxymethyllysine were observed in DG rats when compared to those in CG rats. The final weight was significantly lower in the DG rats than in the CG rats. Histomorphometric evaluation depicted a small quantity of collagen fibers in ligaments and articular cartilage in DG rats, as well as increased collagen in synovial tissue. There was a decrease in cartilage proteoglycans in DG rats when compared with CG rats. Immunofluorescence staining revealed an increase of collagen III and V in ligaments, collagen XI in cartilage, and collagen I in synovial tissue of DG rats compared with CG rats. Conclusion: The ligaments, cartilage and synovia are highly affected following STZ-induced diabetes in rats, due the remodeling of collagen types in these tissues. This process may promote the degradation of the extracellular matrix, thus compromising joint function. Our data may help to better understand the pathogenesis of joint involvement related to diabetes.2012-01-01T00:00:00ZDistribution of exogenous metallothionein following intraperitoneal and intramuscular injection of metallothionein-deficient miceLewis, Katherine E. LewisChung, Roger S.West, Adrian K.Chuah, Meng Innhttp://hdl.handle.net/10201/544642020-02-26T01:22:46Z2012-01-01T00:00:00ZTítulo: Distribution of exogenous metallothionein following intraperitoneal and intramuscular injection of metallothionein-deficient mice
Autor/es principal/es: Lewis, Katherine E. Lewis; Chung, Roger S.; West, Adrian K.; Chuah, Meng Inn
Resumen: Metallothionein-I/II (MT-I/II) is a small metal-binding protein with antioxidant and neuroprotective properties, which has been used experimentally as a neurotherapeutic agent in multiple conditions. Therefore it is important to determine whether exogenous MT-I/II is retained in specific organs or expelled from the body following intramuscular and intraperitoneal injection. The distribution of exogenous MT-IIA (the major human MT-I/II isoform) was examined in MT-I/II-deficient mice, by immunohistochemistry of tissue samples and western blotting of urine samples. MT-IIA was detected within epithelial cells of the kidney cortical and medullary tubules within 1 hour of either intramuscular or intraperitoneal injection. Additionally, MT-IIA was detected within the urine at 1 hour after injection, indicating rapid absorbance into the circulation and filtration through the kidney glomerulus. A portion of the intramuscularly-injected MT-IIA remained within the muscle for at least 24 hours after injection. No MT-IIA was observed within the liver or the brain after either a single injection or a series of MT-IIA injections. These results are consistent with earlier reports that exogenously administered MT-IIA does not cross the intact blood-brain barrier, although a receptor for MT-I/II (megalin) is present in the choroid plexus. We postulate that due to losses through the urine, circulating MT-IIA levels drop rapidly after injection and do not permit transport across the choroid plexus. Peptide analogues of MT-I/II with similar neuroactive properties (emtins) may be more suited for CNS delivery.2012-01-01T00:00:00Z