Digitum Colección:http://hdl.handle.net/10201/1169632024-03-28T15:25:10Z2024-03-28T15:25:10ZSialic acid expression in human fetal skeletal muscle during limb early myogenesisMarini, MircaSarchielli, EricaZappoli Thyrion, Giorgia DonataAmbrosini, StefanoSgambati, Eleonorahttp://hdl.handle.net/10201/1179232022-03-12T00:16:22Z2017-01-01T00:00:00ZTítulo: Sialic acid expression in human fetal skeletal muscle during limb early myogenesis
Autor/es principal/es: Marini, Mirca; Sarchielli, Erica; Zappoli Thyrion, Giorgia Donata; Ambrosini, Stefano; Sgambati, Eleonora
Resumen: Investigations on animal models
demonstrated that changes of sialic acid (SA)
expression, particularly the polymeric form, in the
skeletal muscle during embryonic and post-natal
development seem to be related to muscle differentiation
and functionality onset. The aim of this study was to
evaluate the monomeric and polymeric SA expression in
human skeletal muscle during early stages of fetal
development, when important morphofunctional events
occur. Specimens of fetal skeletal muscle from limb,
between 9 and 12 weeks of gestation (wg), were
obtained from 19 pregnant women. To investigate some
morphofunctional features occurring during this
development period, haematoxylin-eosin staining, tunel
assay and immunohistochemistry for connexin-43
(Cx43) and parvalbumin were performed. SA expression
and characterization was evaluated using lectin
histochemistry (MAA, SNA, PNA, SBA, DBA),
associated with enzymatic and chemical treatments.
Polysialic acid (PSA) expression was also evaluated
using immunohistochemistry. The results showed
apoptotic myotubes between 9 and 10.5 wg,
disappearing from 11 wg; Cx43 was more abundant in
myotubes/myoblasts between 9 and 9.5 wg, decreasing
and/or disappearing from 10 wg and parvalbumin was
present in myotubes between 10 and 10.5 wg. PSA was
revealed in myotubes/myoblasts from 9 to 10.5 wg; from
11 wg it was reduced or disappeared. Monomeric SA
appeared in myotubes/myoblasts from 10 wg, increasing
successively; acetylated SA was present from 11 wg.
These findings demonstrated that changes in expression
of various types of SA, occurring in human fetal skeletal
muscle during early development, seem to be related to
some morphofunctional aspects distinctive of this
organogenesis crucial period.2017-01-01T00:00:00ZAssessment of immunologic, proangiogenic and neurogenic properties of human peripheral nerve epineurium for potential clinical applicationKlimczak, AleksandraSiemionow, MariaFutoma, KatarzynaJundzill, ArkadiuszPatrzalek, Dariuszhttp://hdl.handle.net/10201/1179052022-03-12T00:12:36Z2017-01-01T00:00:00ZTítulo: Assessment of immunologic, proangiogenic and neurogenic properties of human peripheral nerve epineurium for potential clinical application
Autor/es principal/es: Klimczak, Aleksandra; Siemionow, Maria; Futoma, Katarzyna; Jundzill, Arkadiusz; Patrzalek, Dariusz
Resumen: The epineural sheath is a promising naturally
occurring material for enhancement of peripheral nerve
regeneration. Based on a literature search there is a
limited number of reports on the biological and
immunological properties of human epineurium. The
goal of this study was to assess, using immunocytochemical methods, the immunological (HLA class I and
II antigens, T lymphocytes, macrophages), proangiogenic (VEGF, CD31), and neurogenic (GFAP, S-100)
properties of human epineurium isolated from
ilioinguinal nerves (n=19) taken from deceased donors,
and from sciatic nerves (n=12) taken from limbs
amputated due to critical ischemia. Our studies
confirmed reduced expression of HLA class II antigens
on the infiltrating cells, a reduced number of T
lymphocytes, and greater vessel density in the
epineurium obtained from deceased organ donors.
Macrophages were more abundant in the epineurium
isolated from the amputated limbs. We found that the
epineurium harvested from peripheral nerves of the
deceased donors showed negligible immunogenic and
increased proangiogenic properties compared to the
epineurium of nerves taken from amputated limbs. These
findings support the rationale to use human epineurium
obtained from deceased donors as a new biological
material for enhancement of peripheral nerve repair for
potential clinical application in regenerative medicine.2017-01-01T00:00:00ZEffect of estradiol on the expression of angiogenic factors in epithelial ovarian cancerValladares, MacarenaPlaza Parrochia, FranciscaLépez, MacarenaLópez, DanielaGabler, FernandoGayan, PatricioSelman, AlbertoVega, MargaritaRomero, Carmenhttp://hdl.handle.net/10201/1178862022-03-11T00:09:19Z2017-01-01T00:00:00ZTítulo: Effect of estradiol on the expression of angiogenic factors in epithelial ovarian cancer
Autor/es principal/es: Valladares, Macarena; Plaza Parrochia, Francisca; Lépez, Macarena; López, Daniela; Gabler, Fernando; Gayan, Patricio; Selman, Alberto; Vega, Margarita; Romero, Carmen
Resumen: Introduction: Ovarian cancer presents a high
angiogenesis (formation of new blood vessels) regulated
by pro-angiogenic factors, mainly vascular endothelial
growth factor (VEGF) and nerve growth factor (NGF).
An association between endogenous levels of estrogen
and increased risk of developing ovarian cancer has been
reported. Estrogen action is mediated by the binding to
its specific receptors (ERα and ERβ), altered ERα/ERβ
ratio may constitute a marker of ovarian carcinogenesis
progression. Objective: To determine the effect of
estradiol through ERα on the expression of NGF and
VEGF in epithelial ovarian cancer (EOC). Methodology:
Levels of phosphorylated estrogen receptor alpha
(pERα) were evaluated in well, moderate and poorly
differentiated EOC samples (EOC-I, EOC-II, EOC-III).
Additionally, ovarian cancer explants were stimulated
with NGF (0, 10 and 100 ng/ml) and ERα, ERβ and
pERα levels were detected. Finally, human ovarian
surface epithelial (HOSE) and epithelial ovarian cancer
(A2780) cell lines were stimulated with estradiol, where
NGF and VEGF protein levels were evaluated. Results:
In tissues, ERs were detected being pERα levels
significantly increased in EOC-III samples compared
with EOC-I (p<0.05). Additionally, ovarian explants
treated with NGF increased pERα levels meanwhile
total ERα and ERβ levels did not change. Cell lines
stimulated with estradiol revealed an increase of NGF
and VEGF protein levels (p<0.05). Conclusions:
Estradiol has a positive effect on pro-angiogenic factors
such as NGF and VEGF expression in EOC, probably
through the activation of ERα; generating a positive loop
induced by NGF increasing pERα levels in epithelial
ovarian cells.2017-01-01T00:00:00ZExpression of DNA methylation-related proteins in invasive lobular carcinoma of breast: comparison to invasive ductal carcinomaCha, Yoon JinKim, Hye MinKoo, Ja Seunghttp://hdl.handle.net/10201/1178672022-03-11T00:14:46Z2017-01-01T00:00:00ZTítulo: Expression of DNA methylation-related proteins in invasive lobular carcinoma of breast: comparison to invasive ductal carcinoma
Autor/es principal/es: Cha, Yoon Jin; Kim, Hye Min; Koo, Ja Seung
Resumen: Purpose: We aimed to compare the
expression of DNA methylation-related proteins in
invasive lobular carcinoma (ILC) of breast with those of
invasive ductal carcinoma (IDC) of breast and to assess
its potential clinical application.
Methods: Immunohistochemical staining of DNA
methylation-related proteins (5-meC, DNMT1,
DNMT3B and ISL-1) was applied to tissue microarrays
generated from 108 ILCs and 203 IDCs. Protein
expression and its correlation with clinicopatholgic
variables were statistically analyzed.
Results: ISL-1 and DNMT3B were highly expressed
in ILC (p<0.001) and tumoral 5-meC was highly
expressed in IDC (p=0.006). DNMT1 (p<0.001) showed
higher expression rate in luminal A type ILC. ISL-1 and
DNMT3B showed higher expression rate in both luminal
A type and luminal B type of ILC (p<0.05). In IDC,
tumoral 5-meC commonly showed high positivity
(p=0.039). On univariate analysis, shorter disease-free
survival of ILC was associated with DNMT1 high
positivity (p=0.001) and ISL-1 positivity (p=0.018).
Conclusion: DNA methylation-related proteins are
differentially expressed in ILC and IDC, and DNMT1,
DNMT3B and ISL-1 show high expression rate in ILC.2017-01-01T00:00:00Z