Digitum Comunidad: E094
E094
http://hdl.handle.net/10201/1163
2024-03-29T06:17:16Z
2024-03-29T06:17:16Z
Pig in vitro fertilization: where are we and where do we go?
Romar, Raquel
Cánovas, Sebastián
Matás, Carmen
Gadea, Joaquín
Coy, Pilar
http://hdl.handle.net/10201/139287
2024-02-13T00:11:55Z
2019-01-01T00:00:00Z
Título: Pig in vitro fertilization: where are we and where do we go?
Autor/es principal/es: Romar, Raquel; Cánovas, Sebastián; Matás, Carmen; Gadea, Joaquín; Coy, Pilar
Resumen: The pig is an important livestock animal. Biotechnological interest in this species has increased due to its use, among others, in the generation of transgenic animals for use in biomedicine based on its greater physiological proximity to the human species than other large domestic animals. This development has paralleled an improvement in Assisted Reproduction Techniques (ART) used for this species. However, the ability to generate animals from embryos produced entirely in vitro is still limited and a wide margin for improvement remains. Here we review the procedures, additives, and devices used during pig in vitro fertilization (IVF), focusing on the main points of each step that have offered the best results in terms of increased efficiency of the system. The lack of standardized protocols and consensus on the parameters to be assessed makes it difficult to compare results across different studies, but some conclusions are drawn from the literature. We anticipate that new physiological protocols will advance the field of swine IVF, including induction of prefertilization ZP hardening with oviductal fluid, sperm preparation by swim-up method, increased viscosity through the addition of inert molecules or reproductive biofluids, and the incorporation of 3D devices. Here we also reflect on the need to expand the variables on which the efficiency of pig IVF is based, providing new parameters that should be considered to supply more objective and quantitative assessment of IVF additives and protocols.
Descripción: ©2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Accepted version of a Published Work that appeared in final form in Theriogenology. To access the final edited and published work see https://doi.org/10.1016/j.theriogenology.2019.05.045
2019-01-01T00:00:00Z
p38 inhibitors for the treatment and prophylaxis of live cancer
Sabio, Guadalupe
Tomas-Loba, Antonia
Martinez Gil, Ana
Gil Ayuso-Gontan, Carmen
Gonzalez-Teran, Barbara
Manieri, Elisa
http://hdl.handle.net/10201/139267
2024-02-15T13:20:57Z
2016-01-01T00:00:00Z
Título: p38 inhibitors for the treatment and prophylaxis of live cancer
Autor/es principal/es: Sabio, Guadalupe; Tomas-Loba, Antonia; Martinez Gil, Ana; Gil Ayuso-Gontan, Carmen; Gonzalez-Teran, Barbara; Manieri, Elisa
Resumen: The present invention provides p38 inhibitors which are useful for the treatment and/or prophylaxis of liver cancer.
In particular, the present invention provides compounds capable of inhibiting the intracellular expression of the p38 gamma protein
in the hepatocytes of a subject relative to that observed in the absence of the compound, for use in the therapeutic treatment of liver
cancer.
Descripción: International Publication Number: WO 2016/198698 A3
(WIPO/PCT)
2016-01-01T00:00:00Z
Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant
placental expression of PEG3 and LUM
París-Oller, E.
Navarro-Serna, S.
Soriano-Úbeda, C.
Lopes, J.S.
Matás, C.
Ruiz, S.
Latorre, L.
López-Albors, O.
Romar, R.
Cánovas, S.
Coy, P.
http://hdl.handle.net/10201/139234
2024-02-13T00:12:04Z
2021-01-01T00:00:00Z
Título: Reproductive fluids, used for the in vitro production of pig embryos, result in healthy offspring and avoid aberrant
placental expression of PEG3 and LUM
Autor/es principal/es: París-Oller, E.; Navarro-Serna, S.; Soriano-Úbeda, C.; Lopes, J.S.; Matás, C.; Ruiz, S.; Latorre, L.; López-Albors, O.; Romar, R.; Cánovas, S.; Coy, P.
Resumen: Background: In vitro embryo production (IVP) and embryo transfer (ET) are two very common assisted reproductive technologies (ART) in human and cattle. However, in pig, the combination of either procedures, or even their use separately, is still considered suboptimal due to the low efficiency of IVP plus the difficulty of performing ET in the long and contorted uterus of the sow. In addition, the potential impact of these two ART on the health of the offspring is unknown. We investigated here if the use of a modified IVP system, with natural reproductive fluids (RF) as supplements to the culture media, combined with a minimally invasive surgery to perform ET, affects the output of the own IVP system as well as the reproductive performance of the mother and placental molecular traits.
Results: The blastocyst rates obtained by both in vitro systems, conventional (C-IVP) and modified (RF-IVP), were similar. Pregnancy and farrowing rates were also similar. However, when compared to in vivo control (artificial insemination, AI), litter sizes of both IVP groups were lower, while placental efficiency was higher in AI than in RF-IVP. Gene expression studies revealed aberrant expression levels for PEG3 and LUM in placental tissue for C-IVP group when compared to AI, but not for RF-IVP group.
Conclusions: The use of reproductive fluids as additives for the culture media in pig IVP does not improve reproductive performance of recipient mothers but could mitigate the impact of artificial procedures in the offspring.
Descripción: ©2021. This manuscript version is made available under the CC-BY 4.0 license http://creativecommons.org/licenses/by /4.0/
This document is the Published, version of a Published Work that appeared in final form in Journal of Animal Science and Biotechnology. To access the final edited and published work see https://doi.org/10.1186/s40104-020-00544-0
2021-01-01T00:00:00Z
Growth analysis and blood profile in piglets born by embryo transfer
París-Oller, Evelyne
Matás, Carmen
Romar, Raquel
Lopes, Jordana S.
Gadea, Joaquín
Cánovas, Sebastián
Coy, Pilar
http://hdl.handle.net/10201/139265
2024-02-13T00:12:03Z
2022-01-01T00:00:00Z
Título: Growth analysis and blood profile in piglets born by embryo transfer
Autor/es principal/es: París-Oller, Evelyne; Matás, Carmen; Romar, Raquel; Lopes, Jordana S.; Gadea, Joaquín; Cánovas, Sebastián; Coy, Pilar
Resumen: Assisted reproductive technologies (ART), besides solving several reproductive problems, it has also been used as a tool to improve the animal productivity that is required for feeding the human population. One of these
techniques, the embryo transfer (ET), has presented limitations in the porcine species, which could constrain its
use in the porcine industry. To clarify the potential of this technique, we aimed to compare the impact of using
ET or artificial insemination (AI) on the phenotype of the offspring during its first days of age, in terms of growth
and blood parameters. At birth, the body weight was higher for ET-females than AI-females, but this difference
was no longer observed at day 15. On day 3, it was observed a higher concentration of red blood cells, haemoglobin, and haematocrit in females-ET and a higher concentration of white blood cells in both ET-derived
piglets (males and females) when compared to AI groups. On day 3, the biochemical analysis showed a higher
level of albumin for ET-derived males, and a lower level of bilirubin for ET-females than AI controls. However, all
values were within the normal ranges. Our results indicate that piglets derived from ET seem to be phenotypically similar to those born by AI, which provides preliminary evidence that the ET procedure is a safe technique, but additional studies beyond 15 days of life are requested to conclude its global impact. Furthermore, the presented reference values of blood parameters in this species are interesting data for the pig industry.
Descripción: ©2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
This document is the Published, version of a Published Work that appeared in final form in Research in Veterinary Science. To access the final edited and published work see https://doi.org/10.1016/j.rvsc.2021.11.010
2022-01-01T00:00:00Z