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https://doi.org/10.1016/j.febslet.2005.08.038
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Título: | The Influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsA |
Fecha de publicación: | 26-sep-2005 |
Editorial: | Elsevier |
Cita bibliográfica: | FEBS Letters, 2005, Vol. 579, Issue 23,pp. 5199-5204 |
ISSN: | Print: 0014-5793 Electronic: 1873-3468 |
Palabras clave: | Infrared spectroscopy Amide I′ and II bands Thermal denaturation |
Resumen: | The lack of a membrane environment in membrane protein crystals is considered one of the major limiting factors to fully imply X-ray structural data to explain functional properties of ion channels [Gulbis, J.M. and Doyle, D. (2004) Curr. Opin. Struct. Biol. 14, 440–446]. Here, we provide infrared spectroscopic evidence that the structure and stability of the potassium channel KcsA and its chymotryptic derivative 1–125 KcsA reconstituted into native-like membranes differ from those exhibited by these proteins in detergent solution, the latter taken as an approximation of the mixed detergent-protein crystal conditions. |
Autor/es principal/es: | Encinar Hidalgo, José Antonio Molina Gallego, María Luisa Poveda Larrosa, José Antonio Barrera Olivares, Francisco Nicolás Renart Pérez, María Lourdes Fernández Carvajal, Asia María González Ros, José Manuel |
Facultad/Departamentos/Servicios: | Facultades, Departamentos, Servicios y Escuelas::Departamentos de la UMU::Bioquímica y Biología Molecular "B" e Inmunología |
Versión del editor: | https://www.sciencedirect.com/science/article/pii/S0014579305010380 |
URI: | http://hdl.handle.net/10201/142859 |
DOI: | https://doi.org/10.1016/j.febslet.2005.08.038 |
Tipo de documento: | info:eu-repo/semantics/article |
Número páginas / Extensión: | 6 |
Derechos: | info:eu-repo/semantics/embargoedAccess |
Descripción: | © 2005 Federation of European Biochemical Societies. This document is the Published version of a Published Work that appeared in final form in FEBS Letters. To access the final edited and published work see https://doi.org/10.1016/j.febslet.2005.08.038 |
Aparece en las colecciones: | Artículos: Bioquímica y Biología Molecular "B" e Inmunología |
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