Histology and histopathology Vol. 5, nº 1 (1990)

Ir a Estadísticas

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http://hdl.handle.net/10201/177531

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    Ultrastructure of the parathyroid gland
    (Murcia : F. Hernández, 1990) Hideo Isono; Shizuko Shoumura; Shoichi Emura
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    Canine capillary formation in vitro
    (Murcia : F. Hernández, 1990) Logothetou-Rella, H.; Nesland, Jahn M.; Kipiotis, D.; Paraschou, E.; Sechas, M.; Varonos, D.D.
    Microvascular endothelial cells derived from canine subcutaneous adipose tissue formed knob-like and tube-like structures in vitro without tumorconditioned medium or special substrate. The knob-like structures consisted of acidic and basic glycosaminoglycans arranged in order. Knob-like structures were built from cell extrudates and were responsible for capillary lumen formation in vitro. Transmission electron microscopy confirmed the endothelial nature of the cells which expressed extensive phagolysosomal activity.
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    Vasopressinergic neurons and the associated blood vessels in the rat anterior hypothalamus, an immunohistochemical study
    (Murcia : F. Hernández, 1990) Ray, Prajnan K.; Roy Choudhury, S.
    The paraventricular (PVN) and supraoptic (SON) nuclei of the hypothalamic neurosecretory system have been extensively investigated by many workers. The functional aspects of vasopressin secretion (elaborated by the PVN and SON neurons) in relation to the vasculature of the anterior hypothalamus are also well documented. However, the available data concerning vasopressin (VP) functions are largely based on physiological studies. Corroborative morphological correlation with regard to this has received little attention. The present report elucidates the intricate anatomical relationships between the VP-neurons and the adjoining capillaries in the rat anterior hypothalamus. A peroxidase-antiperoxidase (PAP) immunocytochemical study, using a commercial VP antibody, was carried out for this purpose. The observations are interpreted from a functional standpoint. VP-immunostained elements, i.e. the somata and the processes (mainly dendrites), were localized (i) close to the wall, (ii) on the endothelium, and (iii) occasionally, in the lumen of the hypothalamic capillaries. The findings provide immunocytochemical evidence that the vasopressinergic elements are in direct relationship with the hypothalamic vasculature. This raises some interesting possibilities for the former to be involved in: (i) affecting the permeability of the blood-brain barrier for transport of various nutrient substances (important in aging and Alzheimer's disease), (ii) inducing an alteration in the water permeability of the brain vessels on which depends the precise adjustment of brain water content and of brain volume (fundamental to normal functioning of the brain), and (iii) serving as osmoreceptors of the blood flowing through the capillaries and thus providing a feedback mechanism for VP modulation.
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    Frog stomach enteric plexuses in culture, isolation, morphological characterization and bioelectrical recordings
    (Murcia : F. Hernández, 1990) Azanza, M.J.; Peg, M.T.; Junquera, C.; Aisa, J.; Martínez Ciriano, C.; Blasco, J.
    We have succeeded in the isolation, culture and morphological characterization of Rana ridibunda stomach enteric plexuses. We have furthermore obtained intra and extracellular bioelectric recordings from the explants in culture. The cultare medium used (Eagle MEM), the collagenase digestion and the general culture conditions followed are similar to those applied to mammal enteric plexus explant cultures. The most striking difference is that the solutions were diluted to 70% in order to maintain the osmolar conditions required by the amphibian cells. Acetylcholinesterase, osmium tetroxide-zinc iodide- and para-formaldehydeinduced fluorescence methods reveal similar morphological images from the perivascular fibre plexuses. The different cell types observed by phase contrast light microscopy from the myenteric explants in culture have been identified by comparison with those revealed by the acetylcholinesterase method. The prevailing neurons show piramidal somas; other neurons are bipolar with oval somas and a third type shows oval somas tightly aligned, following sinusoidal courses. The intra and extracellular bioelectric recordings from the explants in culture show that the culture conditions we have applied preserve the electrophysiological properties of the neuronal membranes. These preliminary recordings will allow us to undertake the synaptic characterization of the gastrointestinal neurotransmitters in frogs.
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    Subcutaneous fat necrosis of newborn children
    (Murcia : F. Hernández, 1990) Salas Valién, J.S.; Ribas Ariño, M.T.; Egido Romo, M.; Palau Benavides, M.T.
    We report a case of subcutaneous fat necrosis of a newborn child which appeared 9 days after birth and was cured without any complications. We propose the reclassification of the etiological factors accompanying this lesion by classifying them in a plurietiological syndrome with some basic or essential factors and other causative factors.