Histology and histopathology Vol. 2, nº 1 (1987)

Ir a Estadísticas

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    Scanning electron microscopy of the aggregation of head mesoderm cells from chick embryos
    (Murcia : F. Hernández, 1987) Chamorro, C.A.; Paz, P. de; Villar, J.M.; Fernandez, M
    Head mesoderm cells from chick embryos at different stages of development were dissociated and cultured on plastic coverslips. In all cultures several cellular aggregates were described by means of scanning electron microscopy. Isolated cells present filopodia and lamellipodia. However, when mesoderm cells make contact with one another the filopodial and lamellipodial activity in the contact cellular edge disappear. Thus, the cells into cellular clusters do not present projections. The clusters were circular and bidimensional in character. The scanning electron microscopic observations showed that it is the type 1 variant of "contact inhibition of locomotion" which occurs. By means of these mechanisms the bidimensional aggregates are formed and cellular overlapping is not present. Since the behaviour of the mesoderm cells "in vitro" in some way could be comparable to their behaviour "in situ", the results here observed are discussed in relation to the conduct of mesoderm cells "in vivo".
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    Muscle fiber types in tetrapods. A comparative histochemical and morphometric study
    (Murcia : F. Hernández, 1987) Burgos Bretones, Juan J.; Razquin Peralta, Blanca.; Aguirre Urizar, José; Rivera Pomar, José M.
    A comparative histochemical and morphometric study in two groups of homologous muscles from different tetrapods (rat, pigeon, lizard and frog) was performed. On the basis of their fiber diameters and oxidative enzyme activities, an initial correlation between fiber types of al1 animals is observed, although in the lizard and frog muscles, another fiber type does exists that could not be demonstrated in higher vertebrates. When more than one histochemical techniques are used for the identification of each tetrapod fiber types, the lack of correlation between them becomes obvious. Thus, different animals groups, each showing a characteristic muscle metabolic pattern, could be distinguished.
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    Phase-contrast microscopy of the primate retina
    (Murcia : F. Hernández, 1987) Fix, James D.
    The study of hematoxylin and eosin stained thick sections (15 pm) of the primate retina with the phasecontrast microscope provided a means for the selective demonstration of many cellular structures that could not be resolved with the same degree of detail which was possible when bright-field microscopy was used, or when phase-contrast microscopy was employed to examine unstained material. The H & E-stain greatly enhanced the phase-contrast image, so that cytoplasmic structure, fiber trajectories, and gross synaptic detail of the retina could be demonstrated to better advantage.
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    Studies on intra-arterial cushions. IV. Perivascular nerve plexuses of ramifying arteries with intraluminal cushions at the branching points
    (Murcia : F. Hernández, 1987) Böck, P.
    Periarterial nerve plexuses were studied at branching points of arteries with intraluminal cushions. Serial sections were prepared from mouse kidney, pancreas and tongue, and studied by means of catecholamine fluorescence and staining for acetylcholinesterase. The periarterial nerve plexuses did not show any peculiarities at the branching points. The intraluminal cushions, as well as the tunica media of both the parent trunk and the collateral branches, were found to be free of vegetative nerves. It is concluded that the shape of intraarterial cushions is passively altered, following the alterations of vascular geometry, and not in response to direct nervous stimulation.