Histology and histopathology Vol. 3, nº 4 (1988)

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http://hdl.handle.net/10201/177572

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Effects of long-term hypothyroidism in the morphology and synaptic organization of cerebellar ectopic granule cells
(Murcia : F. Hernández, 1988) Madeira, M.D.; Azevedo, F.P.; Paula-Barbosa, M.M.
Abundant ectopic granule cells scattered in the cerebellar molecular layer have been observed in 30- day-old hypothyroid rats. Their morphological features indicate that they must be regarded as mature heterotopic cells arrested during their migration towards the granular layer. As their impoverished dendritic trees are identical to those seen in controls, it is unlikely that the lack of thyroid hormones played a major role in the deficient dendritic outgrowth. The study of 180-day-old hypothyroid rats revealed that although ectopic granule cells remained quite numerous, their number per unit surface was lesser than in the 30-day-old hypothyroid group. This finding may be related to the capacity displayed by heterotopic neurons to establish synaptic contacts with the components of the molecular layer. This was inferred by the presente of a peculiar synaptic cell investment formed by axosomatic and somatodendritic contacts in 180-day-old hypothyroid rats which shows that the surviving ectopic granule cells manage to adapt to an adverse milieu.
Open Access
Effects of relaxin on the microvasculature of mouse mammary gland
(Murcia : F. Hernández, 1988) Bani, Gastone; Bani-Sacchi, Tatiana; Bigazzi, Mario; Bianchi, Stefano
The effects of RLX on the microvasculature of the mouse mammary gland are reported. RLX (pure porcine standard NIH-RXN-P1) at a dose of 3 GPU was administered subcutaneously to virgin adult mice ovariectomized 12 days before. The mammary glands were removed 18-20 h after RLX injection and their examination by light microscopy did not reveal any substantial growth-response to the hormone . Histology and morphometry indicated striking dilation of microvessels, especially capillaries. and electron microscopy revealed an increase in the micropinocytotic vesicles, thus suggesting enhanced transendothelial transport of substances. Such phenomena, which were independent of a release of granules by mast cells, may represent an important component of the mammotrophic action of RLX.
Open Access
Leiomyosarcomas, Three cases with desmin positive tumour cells, lacking ultrastructural features of smooth muscle cells
(Murcia : F. Hernández, 1988) Roholl, Paul J.M.; De Jong, Anton S.H.; Albus-Luttei, Cherry E.; van Unnik, Jan A.M.
A combined study of light and electron microscopy and of immunolabelling of three pleomorphic spindle cell sarcomas is presented. The light and electron microscopic features of these sarcomas were most compatible with those described for malignant fibrous histiocytoma (MFH, pleomorphic-storiform subtype). Electronmicroscopically undifferentiated and fibroblastlike cells, fibrohistiocytes and multinucleated histiocytes were observed. Characteristics belonging to smooth - - muscle cells were absent. By immunostaining, vimentin and desmin could be obseived in tumour Glls of al1 three cases, at least on frozen sections. Other markers such as alpha,- antichymotrypsin, S-100 proteins, laminin. collagen IV and markers specific for skeletal muscle cells (myoglobin, actin and myosin specific for skeletal muscle) could not be demonstrated. These findings indicate that three MFH's are, in fact, poorly differentiated variants of smooth muscle tumours. It is concluded that immunophenotyping is very useful for this type of neoplasm.
Open Access
Neuroendocrine lung structures and tumours, immunohistochemical study by specific markers
(Murcia : F. Hernández, 1988) Mosca, L.; Barbareschi, M.; Mauri, M.F.; Munguia-Barrera, J.; Frigo, B.; Muscara, M.; Valentini, L.; Mariscotti, C.
Out of 360 lungs or lobes surgically removed, 13 non neoplastic specimens and 16 neuroendocrine (NE) tumours are investigated with immunohistochemical methods, in order to evaluate the presence of NE structures in normal and pathological human lungs. The markedused are neuron specific enolase (NSE), chromogranin (CG) and the 80 kd antigen (80 kdAg) of NE secretory granules detected by the new monoclonal Phe-5 antibody. In nonneoplastic lung specimens, clearcut immunoreactivity for al1 three markers appears in NE cells, neuroepithelial bodies (NEB), NE cell-hyperplasias and dysplasias. In the same specimens 4 tumourlets with analogous clearcut immunoreactivities were also observed. The NE tumours show distinct immunoreactivity for al1 three antisera in the 8 well differentiated cases. The 8 poorly differentiated tumours are variably immunoreactive for NSE and present low to ni1 staining with antisera to CG and 80 kdAg. The immunohistochemical data are interpreted according to current views about a possible relationship between NE tumours and parent normal NE lung structures.
Open Access
The lectin binding pattern of normal and pathologically altered synovial tissue
(Murcia : F. Hernández, 1988) Zschabitz, A.; Stofft, E.
Light-microscopical lectin-binding studies were carried out in healthy and pathologically altered synovial tissue (osteoarthrosis, rheumatoid arthritis (RA)). Seven lectins were studied: Con A, DBA, PNA, RCA, SBA, UEA-1, and WGA. Con A and WGA mark al1 lining cells and the majority of subintimal synovial cells. RCA and SBA stain only a portion of lining cells, regardless of the basic pathology. The lectin PNA reacts only with RA and arthrotic material, and is thus suitable for the diagnosis of inflammatory changes in synovial tissue. UEA-1 is a consistent marker for capillary endothelium and large vessels.

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