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El objetivo de esta comunidad es poner a disposición de los usuarios aquellos materiales generados o recolectados durante el transcurso de una investigación.
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Browsing Datos de Investigación by browse.metadata.subjectods "Objetivo 14: Océanos"
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PublicationOpen AccessBiomarker-based assessment of somatostatin-6 immunomodulation in gilthead Seabream: From serum proteins to skin mucus enzymes(0022-10-20) Campos Sánchez, José Carlos; Espinosa Ruiz, Cristóbal; Marín Parra, Claudia; Guardiola Abellán, Francisco Antonio; Esteban Abad, María Ángeles; Biología Celular e HistologíaThis study evaluated the immunomodulatory role of somatostatin-6 (SST6) in gilthead seabream (Sparus aurata) using a validated inflammation model induced by λ-carrageenan and a comprehensive panel of serum and skin mucus biomarkers. SST6 was administered at 1 nM and 2 nM, and its effects were assessed in these matrices. The λ-carrageenan model triggered a local inflammatory response with reduced peroxidase, esterase, and protease activities in skin mucus, consistent with the resolution phase of innate immunity. SST6 at 1 nM enhanced serum complement activity without affecting acute-phase proteins, suggesting reinforcement of basal immune surveillance in the host. When combined with λ-carrageenan, SST6 promoted an increase in serum immunoglobulin levels and a selective shift in proteinogram fractions (α2-globulin), while further decreasing the oxidative and proteolytic activities in skin mucus. Correlation analysis revealed the coordinated regulation of key mucosal enzymes, reinforcing the functional integration of local immune responses. These results indicate that SST6 facilitates an early transition from innate to adaptive immunity while preserving the integrity of the mucosal barrier. Furthermore, this study provides the first evidence of complement activation by SST6 in fish and highlights the value of integrating biomarker-based tools to monitor immunophysiological responses and test bioactive compounds in aquaculture research.- PublicationOpen AccessData research: Validation of quality, perceived value, future practice intention and satisfaction scale of activity charter focused on nautic business development.(0001-01-23) Borrego Balsalobre, Francisco José; Actividad Física y Deporte
- PublicationOpen AccessRadiation shielding potential of Oyster Shells (Ostrea edulis)(2026-07-27) Gómez, Cristina; Esteban Abad, María Ángeles; Martínez López, Salvadora; Arnaldos Sanabria, María Isabel; Esteban Abad, María Ángeles; Biología Celular e Histología; Facultades de la UMU::Facultad de BiologíaEl objetivo de este estudio era analizar la composición elemental y la mineralogía de las conchas de ostras de cultivo (O. edulis, Linnaeus, 1758) y explorar la viabilidad de estas conchas para el desarrollo de biomateriales de protección contra la radiación. Las conchas pueden valorizarse, sustituyendo parcialmente a las fuentes minerales no renovables en algunas aplicaciones actuales, contribuyendo así a la implantación de una economía circular de cero residuos. Además, el uso de carbonato cálcico (CaCO₃) procedente de las conchas en aplicaciones que garanticen su inertización durante largos periodos puede evitar la liberación de dióxido de carbono a la atmósfera, contribuyendo así a la reducción de la huella de carbono de la industria del marisco.
- PublicationOpen AccessResearch data for the article: Differential immunomodulatory effects of entinostat and N1,N8-dihydroxyoctanediamide on gilthead seabream (Sparus aurata) head-kidney leucocytes(Elsevier, 2026) Ortiz García, María; Marín Parra, Claudia; Esteban Abad, María Ángeles; María Ángeles Esteban Abad; Esteban Abad, María Ángeles; Biología Celular e Histología; María Ángeles Esteban Abad; Facultades de la UMU::Facultad de BiologíaHistone deacetylases (HDACs) govern epigenetic control of gene transcription and are increasingly recognised as key regulators of innate and adaptive immunity, making HDAC inhibitors (iHDACs) promising immunomodulatory tools. However, direct evidence of how iHDACs affect teleost leucocyte functions is scarce, particularly in marine aquaculture species. This study investigated, for the first time in gilthead seabream (Sparus aurata), the in vitro concentration- and time-dependent effects of two chemically distinct class I targeting iHDACs: entinostat (E; 0, 5, 10 nM) and N1,N8-dihydroxyoctanediamide (N1N8; 0, 2.5, 5 µM), on head-kidney leucocyte (HKLs) viability and core innate effector functions. HKLs viability remained high and unchanged after incubation with E or N1N8. Incubation of HKLs with E selectively increased phagocytic ability, with 5 nM producing a significantly higher proportion of phagocytic cells than the control and 10 nM, while phagocytic capacity was unaffected. Incubation of HKLs with N1N8 did not alter phagocytosis. Neither compound significantly modified the HKLs respiratory burst. However, HKLs degranulation was significantly enhanced only by 5 µM N1N8. Finally, ETs formation was significantly increased by both E and even more with N1N8, without clear dose dependence. These findings demonstrate non-cytotoxic, function-selective immunomodulation of seabream HKLs by iHDACs, with compound-specific signatures that support further mechanistic and in vivo evaluation for fish health applications, while considering potential inflammatory consequences of enhanced ETs responses.
- PublicationOpen AccessResearch data for the article: Anthropogenic electromagnetic fields and their influence on growth, humoral Immunity, and metal concentrations in gilthead seabream (Sparus aurata)(Elsevier, 2026-01-27) Ortiz García, María; García Gamuz, José Antonio; Martínez López, Salvadora; Esteban Abad, María Ángeles; Biología Celular e Histología; Facultad de BiologíaThe increasing presence of extremely low-frequency electromagnetic fields (ELF-EMF) in marine environments, driven by human activities and climate change, poses a potential risk to aquatic organisms. This study investigated the physiological and immunological effects of ELF-EMF exposure on gilthead seabream (Sparus aurata), a key species in Mediterranean aquaculture. Fish were exposed to ELF-EMF for one or three weeks, and their growth, serum immune parameters (peroxidase and lysozyme activities), and tissue chemical elements were compared with those of unexposed controls. No significant differences were observed in fish weight, length, or serum immune parameters between the exposed and control groups at any time point. However, a significant reduction in liver cadmium (Cd) levels was detected in fish exposed to ELF-EMF for three weeks, suggesting potential modulation of metal detoxification pathways. The concentrations of other elements, including arsenic (As), lead (Pb), iron (Fe), manganese (Mn), copper (Cu), and zinc (Zn), showed minor changes in various tissues. The levels of sodium (Na), magnesium (Mg), calcium (Ca), and potassium (K) were altered in some tissues after one week of ELF-EMF exposure but generally returned to control levels after three weeks. These findings indicate that short-term ELF-EMF exposure does not significantly affect growth or innate immunity in gilthead seabream under the tested conditions. The observed alterations in tissue levels of toxic and trace elements, as well as macronutrients, indicate potential interactions between ELF-EMF, metal homeostasis, and detoxification mechanisms, necessitating further investigation. This study contributes to the understanding of the effects of anthropogenic ELF-EMF on marine organisms and highlights the need for continued research to assess the long-term ecological implications of increasing ELF-EMF levels in aquatic environments.
- PublicationOpen AccessResearch data for the article: Cellular responses to electromagnetic fields and ultraviolet radiation: an in vitro study on microalgae and fish cell lines(Elsevier, 2026) Ortiz García, María; Terrer Marcilla de Teruel Moctezuma, José María; Aboal Sanjurjo, Marina; García Gamuz, José Antonio; Esteban Abad, María Ángeles; Biología Celular e Histología; Facultades de la UMU::Facultad de BiologíaBackground The molecular effects involved in the cellular response to non-ioninzing electromagnetic radiation on marine and freshwater microalgae and fish cell lines are incompletely understood. Objectives The direct impact of non-ionizing electromagnetic radiation on marine and freshwater microalgae and fish cell lines was investigated. Methods Six microalgae species from different taxonomic groups were exposed to extremely low-frequency electromagnetic fields (ELF-EMF) for five days. Four fish cell lines (SAF-1, DLB-1, DLEC, and Fugu eye) were exposed to ultraviolet (UV) radiation, with SAF-1 demonstrating the highest sensitivity. In addition, three commercial antioxidants (2101, 3600, and 4004) were tested for their possible radioprotective effects. Results Although no statistically significant differences in cell density were observed, certain taxa (Chlorotylium sp., Mayamea sp., and Scenedesmus sp.) exhibited a declining trend compared to the controls, suggesting species-specific sensitivity to ELF-EMF. Principal component analysis revealed significant differences in physiological responses, particularly in photosynthetic variables such as electron transport rate and dissolved carbon. The total antioxidant capacity did not differ significantly between the control and exposed microalgae, and Chlorotylium sp. showed the highest basal levels. The photosystem II productivity index remained largely unaffected by ELF-EMF exposure, except for an increase in irradiated Scenedesmus sp. Low antioxidant concentrations (≤125 µg/ml) and short incubation times (6 h) of 2101 and 3600 induced proliferation or increased cell viability, particularly under irradiation. However, prolonged incubation time (24 h) or high antioxidant concentrations (≥250 µg/ml) resulted in significant cytotoxicity in all cell lines.
- PublicationOpen AccessResearch data for the article: Effects of Nicotinamide Mononucleotide and Nicotinamide Riboside on Mitochondrial Bioenergetics and Migration in Gilthead Seabream SAF‑1 Cells(Springer Nature, 2026-09-11) Ortiz García, María; Esteban Abad, María Ángeles; Esteban Abad, María Ángeles; Biología Celular e Histología; Facultades de la UMU::Facultad de BiologíaBackground. Nicotinamide adenine dinucleotide (NAD⁺) regulates redox balance, DNA repair, and stress responses, stimulating interest in NAD⁺ enhancers, nicotinamide mononucleotide (NMN), and nicotinamide riboside (NR). This study investigated NMN and NR in the seabream (Sparus aurata) fibroblast-like cell line SAF‑1 to identify non-cytotoxic conditions and assess their effects on mitochondrial bioenergetics and collective migration, which are relevant to tissue repair and aquaculture applications. Results. The compatibility of the samples with Leibovitz’s L‑15 medium was evaluated. NMN acidified the medium in a concentration-dependent manner (pH 7.33 at 0.16 mM to 5.05 at 40 mM), whereas NR maintained a near-neutral pH (7.15–7.39); both compounds increased osmolarity at concentrations. SAF‑1 viability was maintained with NMN concentrations ≤0.5 mM but decreased with 1 mM over time (approximately 55% at 96 hours). NR was tolerated at 0.16–0.75 mM, with increased viability, whereas higher doses showed delayed cytotoxicity. Bioenergetically, NMN induced modest changes in OCR, with enhanced respiration observed only at 1 mM. Conversely, NR increased basal OCR (0.16–0.75 mM), ATP-linked respiration, and coupling efficiency (notably at 0.38–0.75 mM) without increasing proton leakage, although non-mitochondrial oxygen consumption increased with NR. Both compounds facilitated wound closure, but the migration speed increased only at the lowest doses tested (0.25 mM NMN: ~7–10 µm·h⁻¹; 0.16 mM NR: ~9 µm·h⁻¹). Conclusions. Overall, NR induced a broader and more efficient mitochondrial phenotype in SAF‑1 cells, whereas both NAD⁺ precursors promoted migration at low concentrations. These findings underscore the need to manage medium acidification with NMN and support NAD⁺ modulation as a strategy to enhance the cellular repair capacity in fish-relevant systems.
- PublicationOpen AccessResearch data for the article: NAD⁺-Boosting Precursors Nicotinamide Mononucleotide and Nicotinamide Riboside Enhance Innate Immune Effector Functions in Gilthead Seabream Head-Kidney Leucocytes(2026-09-21) Ortiz García, María; Esteban Abad, María Ángeles; Biología Celular e Histología; Esteban Abad, María Ángeles; Facultades de la UMU::Facultad de BiologíaNAD+-boosting molecules are increasingly being explored as immunometabolic modulators; however, their direct effects on fish innate immune effector functions remain incompletely defined. This study aimed to evaluate the immunomodulatory potential of nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR) in gilthead seabream (Sparus aurata) head kidney leucocytes (HKLs) within a broader in vitro screening framework. HKLs were isolated and incubated with NMN (0.25–0.50 mM) or NR (0.38–0.75 mM) for up to 0–18 h after confirming the pH and osmolarity of the dilutions. Cell morphology was monitored using phase-contrast microscopy.[MO1.1] HKLs viability remained high (~89–93%), with only 0.75 mM NR causing a modest but significant reduction compared to the control (p<0.05). Leucocyte function was assessed sequentially as an integrated innate immunity readout. Both NMN and NR significantly increased phagocytic ability (from ~54% to ~62–71%; p<0.05) without significantly altering the phagocytic capacity. Respiratory burst increased with 0.25 mM NMN (p<0.05) and NR, most clearly at 0.75 mM (p<0.05). Peroxidase activity rose significantly only with NR at 0.75 mM (~0.50 vs. ~0.20 a.u.; p<0.05). Extracellular trap formation was consistently enhanced by both compounds at all tested concentrations (p<0.05). Overall, NMN and NR were well tolerated and potentiated complementary intracellular and extracellular antimicrobial mechanisms, primarily by increasing the fraction of functionally activated cells. Distinct dose sensitivities across endpoints suggest mechanistic compartmentalisation of immunometabolic control.