Browsing by Subject "Sulforaphane"

Now showing 1 - 5 of 5
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    Bioavalability and new biomarkers of cruciferous sprouts consumption
    (Elsevier, 2017-07-21) Baenas, Nieves; Suárez-Martínez, Clara; García-Viguera, Cristina; Moreno, Diego A.; Tecnología de Alimentos, Nutrición y Bromatología
    The evaluation of the bioavailability of cruciferous compounds is one of several challenges in the design of clinical trials. A 7-days-cross-over study with fourteen women was undertaken to compare the bioavailability of glucosinolates from broccoli and radish sprouts. The urinary excretion of isothiocyanates, indoles and metabolites was analysed by UHPLC-QqQ-MS/MS. Even though the bioavailability of broccoli compounds has been studied, as far as we are aware, there are not any biomarkers established for radish sprouts intake. For the first time, sulforaphene, sulforaphane-N-acetyl-L-cysteine (SFN-NAC) and 3,3’diindolylmethane (DIM), were studied as biomarkers of dietary exposure to radish. The SFN-NAC and DIM were already considered biomarkers of broccoli consumption. Higher excretion of conjugated isothiocyanates and homogeneous excretion of indoles were found during the first 12 h after ingestion. Metabolites were excreted homogeneously during the study, suggesting no accumulation. These results provide valuable information to better understand the bioavailability of cruciferous bioactives.
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    Influence of vooking methods on glucosinolates and isothiocyanates content in novel cruciferous foods
    (MDPI, 2019-07-12) Baenas, Nieves; Marhuenda, Javier; García Viguera, Cristina; Zafrilla, Pilar; Moreno, Diego A.; Tecnología de Alimentos, Nutrición y Bromatología
    Brassica vegetables are of great interest due to their antioxidant and anti-inflammatory activity, being responsible for the glucosinolates (GLS) and their hydroxylated derivatives, the isothiocyanates (ITC). Nevertheless, these compounds are quite unstable when these vegetables are cooked. In order to study this fact, the influence of several common domestic cooking practices on the degradation of GLS and ITC in two novel Brassica spp.: broccolini (Brassica oleracea var italica Group x alboglabra Group) and kale (Brassica oleracea var. sabellica L.) was determined. On one hand, results showed that both varieties were rich in health-promoter compounds, broccolini being a good source of glucoraphanin and sulforaphane (≈79 and 2.5 mg 100 g−1 fresh weight (F.W.), respectively), and kale rich in glucoiberin and iberin (≈12 and 0.8 mg 100 g−1 F.W., respectively). On the other hand, regarding cooking treatments, stir-frying and steaming were suitable techniques to preserve GLS and ITC (≥50% of the uncooked samples), while boiling was deleterious for the retention of these bioactive compounds (20–40% of the uncooked samples). Accordingly, the appropriate cooking method should be considered an important factor to preserve the health-promoting effects in these trending Brassica.
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    Membrane vesicles for nanoencapsulated sulforaphane increased their anti-inflammatory role on an In vitro human macrophage model.
    (MDPI, 2022-02-09) Yepes Molina, Lucía; Pérez Jiménez, María Isabel; Martínez Esparza, M.; Teruel, José A.; Ruiz Alcaraz, Antonio J.; García Peñarrubia, Pilar; Carvajal, Micaela; Bioquímica y Biología Molecular B e Inmunología
    At present, there is a growing interest in finding new non‐toxic anti‐inflammatory drugs to treat inflammation, which is a key pathology in the development of several diseases with considerable mortality. Sulforaphane (SFN), a bioactive compound derived from Brassica plants, was shown to be promising due to its anti‐inflammatory properties and great potential, though its actual clinical use is limited due to its poor stability and bioavailability. In this sense, the use of nanocarriers could solve stability‐related problems. In the current study, sulforaphane loaded into membrane vesicles derived from broccoli plants was studied to determine the anti‐inflammatory potential in a human‐macrophage‐like in vitro cell model under both normal and inflammatory conditions. On the one hand, the release of SFN from membrane vesicles was modeled in vitro, and two release phases were stabilized, one faster and the other slower due to the interaction between SFN and membrane proteins, such as aquaporins. Furthermore, the anti‐inflammatory action of sulforaphane‐loaded membrane vesicles was demonstrated, as a decrease in interleukins crucial for the development of inflammation, such as TNF‐α, IL‐1β and IL‐6, was observed. Furthermore, these results also showed that membrane vesicles by themselves had anti‐inflammatory properties, opening the possibility of new lines of research to study these vesicles, not only as carriers but also as active compounds.
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    Potential of Sulforaphane and Broccoli Membrane Vesicles as Regulators of M1/M2 Human Macrophage Activity
    Ramirez Pavez, T. N.; García Peñaranda, A.; Garcia Ibañez, P.; Yepes Molina, L.; Carvajal Alcaraz, M.; Ruiz Alcaraz, A. J.; Moreno Fernández, D.; Garcia Peñarrubia, M. D. P.; Martínez-Esparza, M.; Bioquímica y Biología Molecular B e Inmunología
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    The preventive effects of broccoli bioactives against cancer: Evidence from a validated rat glioma model
    (Elsevier, 2023-10-13) Baenas, Nieves; Vega-García, Angélica; Manjarrez-Marmolejo, Joaquín; Moreno, Diego A.; Feria-Romero, Iris A.
    The aggressive and incurable diffuse gliomas constitute 80% of malignant brain tumors, and patients succumb to recurrent surgeries and drug resistance. Epidemiological research indicates that substantial consumption of fruits and vegetables diminishes the risk of developing this tumor type. Broccoli consumption has shown beneficial effects in both cancer and neurodegenerative diseases. These effects are partially attributed to the isothiocyanate sulforaphane (SFN), which can regulate the Keap1/Nrf2/ARE signaling pathway, stimulate detoxifying enzymes, and activate cellular antioxidant defense processes. This study employs a C6 rat glioma model to assess the chemoprotective potential of aqueous extracts from broccoli seeds, sprouts, and inflorescences, all rich in SFN, and pure SFN as positive control. The findings reveal that administering a dose of 100 mg/kg of broccoli sprout aqueous extract and 0.1 mg/kg of SFN to animals for 30 days before introducing 1 × 104 cells effectively halts tumor growth and progression. This study underscores the significance of exploring foods abundant in bioactive compounds, such as derivatives of broccoli, for potential preventive integration into daily diets. Using broccoli sprouts as a natural defense against cancer development might seem idealistic, yet this investigation establishes that administering this extract proves to be a valuable approach in designing strategies for glioma prevention. Although the findings stem from a rat glioma model, they offer promising insights for subsequent preclinical and clinical research endeavors.