Browsing by Subject "Sperm DNA fragmentation"

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    Associations between urinary concentrations of bisphenol A and sperm DNA fragmentation in young men.
    (Elsevier, 2021-08) Kiwitt Cárdenas, Jonathan; Adoamnei, Evdochia; Arense Gonzalo, Julián J.; Sarabia Cos, Laura; Vela Soria, Fernando; Fernández, Mariana F.; Gonsálvez, Jaime; Mendiola Olivares, Jaime; Torres Cantero, Alberto M.; Ciencias Sociosanitarias
    Background: Bisphenol A (BPA) is one of the most common endocrine disruptor compounds in our environment, promoting a xenoestrogenic state. Numerous studies have shown a relationship between exposure to BPA and male infertility problems. Spermatic DNA integrity is a critical factor for the correct transmission of paternal genetic material to the embryo. However, only a very few studies have investigated the association between urinary BPA concentrations and human sperm DNA fragmentation (SDF). Method: Cross-sectional study conducted with 158 healthy university students (18–23 years), recruited between 2010 and 2011 in the Region of Murcia (Spain). The subjects provided urine and semen samples on a single day. Urinary BPA concentrations were measured by dispersive liquid–liquid microextraction and ultrahigh performance liquid chromatography with tandem mass spectrometry detection, and SDF analysed using the Sperm Chromatin Dispersion test. Statistical analyses were made using linear regression adjusting for potential covariates and confounding factors. Results: No association was found between urinary BPA concentrations and SDF index in the total group. However, in the subgroup of men with SDF index> 30%, significant positive associations across quartiles (ptrend= 0.02) and as a continuous BPA levels were observed (β = 0.055, 95%, CI: 0.002; 0.108). Conclusion: Our results show that, within the subgroup of men with relatively high SDF index, the higher the concentration of BPA the greater the SDF index. Nonetheless, more studies are required to confirm these results and draw conclusions in other male populations.
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    Urinary concentrations of bisphenol A, parabens and benzophenone-type ultra violet light filters in relation to sperm DNA fragmentation in young men: a chemical mixtures approach.
    (Elsevier, 2024-02-20) Kiwitt Cárdenas, Jonathan; Arense Gonzalo, Julián J.; Adoamnei, Evdochia; Sarabia Cos, Laura; Vela Soria, Fernando; Fernández, Mariana F.; Gonsálvez, Jaime; Mendiola Olivares, Jaime; Torres Cantero, Alberto M.; Ciencias Sociosanitarias
    People are daily exposed to multiple endocrine disruptor compounds (EDCs) that may interfere with different molecular and cellular processes, promoting a potential estrogenic, androgenic, or anti-androgenic state. However, most epidemiological studies attempting to establish relationships between EDCs exposure and health effects are still considering individual compounds. A few studies have shown associations between exposure to individual non-persistent EDCs and sperm DNA fragmentation (SDF) in different male populations. Thus, the aim of this study was to investigate associations between combined exposure to non-persistent EDCs and SDF index in young men. A cross-sectional study was conducted with 158 healthy university students from Southeaster Spain. The participants provided spot urine and semen samples on the same day. The concentrations of urinary bisphenol A (BPA), benzophenones [2,4-dihydroxybenzophenone (BP-1); 2,2′,4,4′-tetrahydroxybenzophenone (BP-2), 2-hydroxy-4-methoxybenzophenone (BP-3), 2,2′-dihydroxy-4-methoxybenzophenone (BP-8), 4-hydroxybenzophenone (4OHBP)], and parabens (methylparaben, ethylparaben, propylparaben, butylparaben) were measured by dispersive liquid-liquid microextraction and ultrahigh-performance liquid chromatography with tandem mass spectrometry detection. SDF was analysed using a Sperm Chromatin Dispersion test. Statistical analyses were carried out using Bayesian Kernel Machine Regression models to evaluate associations between combined exposure to these compounds and SDF index while adjusting by relevant covariates. The increase in urinary concentration of 4OHBP was found to be the most important contributor to the negative association between urinary EDCs concentrations and SDF index, being of 􀀀 5.5 % [95 % CI: 􀀀 10.7, 􀀀 0.3] for those in percentile 50, and 􀀀 5.4 % [95 % CI: 􀀀 10.8, 􀀀 0.1] for those in percentile 75. No significant associations were observed between other EDCs and SDF index. Our findings show that urinary 4OHBP levels may be associated with a decrease in the SDF index. Nonetheless, the effects we observed were likely to be small and of uncertain clinical significance. Further research is needed to replicate our findings in other male populations.