Browsing by Subject "Mineral trioxide aggregate"

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    Biocompatibility of a HA/β-TCP/C scaffold as a pulp-capping agent for vital pulp treatment: an in vivo study in rat molars
    (MDPI, 2021-04-08) Guerrero Gironés, Julia; Alcaina Lorente, Antonio; Ortiz Ruiz, Clara; Ortiz Ruiz, Eduardo; Pecci Lloret, María P.; Ortiz Ruiz, Antonio José; Rodríguez Lozano, Francisco Javier; Pecci Lloret, Miguel R.; Dermatología, Estomatología, Radiología y Medicina Física
    Bioceramic materials possess desirable biological properties, highlighting their non-reactivity and osteoconductivity. Their use has been extended in vital pulp treatment. The purpose of this study was to evaluate and compare the effects of beta-tricalcium phosphate (β-TCP), hydroxyapatite (HA), and collagen (C) scaffold with mineral trioxide aggregate (MTA) on the vital pulp of rat molars. Thirty-two molars of Sprague–Dawley rats underwent direct pulp capping with β-TCP/HA/C (n = 16) and MTA (n = 16). After 30 days, the following parameters were evaluated in the tested samples: the degree of pulp inflammation and pulp vitality, the presence of reparative dentin, the homogeneity of the odontoblastic layer, and the presence of pulp fibrosis. No statistically significant differences were observed between HA/β-TCP/C and MTA in terms of the degree of inflammation (p = 0.124). Significant differences were found in reparative dentin formation between the treatment groups (p = 0.0005). Dentin bridge formation was observed in the MTA-treated group. The local action of HA/β-TCP/C is similar to that of MTA when used as an agent for pulp vital treatment in terms of absence of inflammation and maintenance of pulp vitality, although there are significant differences between both materials regarding the formation of dentin bridges.
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    Biocompatibility of new pulp-capping materials NeoMTA Plus, MTA Repair HP and Biodentine on human dental pulp stem cells
    (Elsevier Science, 2017-11-01) Tomás Catalá, Christopher Joseph; Collado-González, Mar; García Bernal, David; Oñate Sánchez, Ricardo Elías; Forner, Leopoldo; Llena, Carmen; Lozano, Adrián; Moraleda Jiménez, José María; Rodríguez Lozano, Francisco Javier; Biología Celular e Histología
    Introduction: The aim of the present study was to evaluate the in vitro cytotoxicity of MTA Repair HP, Neo-MTA Plus, and Biodentine, new bioactive materials used for dental pulp capping, on human dental pulp stem cells (hDPSCs). Methods: Biological testing was carried out in vitro on hDPSCs. Cell viability and cell migration assays were performed using eluates of each capping material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. The chemical composition of the pulp-capping materials was determined by energy-dispersive X-ray and eluates were analyzed by inductively coupled plasma-mass spectrometry. Statistical differences were assessed by analysis of variance and Tukey test (P < .05). Results: Cell viability was moderate after 24 and 48 hours in the presence of MTA Repair HP and NeoMTA Plus, whereas at 48 and 72 hours, Biodentine showed higher rates of cell viability than MTA Repair HP and NeoMTA Plus (P < .001). A cell migration assay revealed adequate cell migration rates for MTA Repair HP and NeoMTA Plus, both similar to the control group rates, meanwhile the highest cell migration rate was observed in the presence of Biodentine (P < .001). Scanning electron microscope studies showed a high degree of cell proliferation and adhesion on Biodentine disks but moderate rates on MTA Repair HP and NeoMTA Plus disks. Energydispersive X-ray pointed to similar weight percentages of C, O, and Ca in all 3 materials, whereas other elements such as Al, Si, and S were also found. Conclusions: The new pulp-capping materials MTA Repair HP, NeoMTA Plus, and Biodentine showed a suitable degree of cytocompatibility with hDPSCs, and good cell migration rates, although Biodentine showed higher rates of proliferation time-dependent.
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    Biological effects of acid-eroded MTA Repair HP and ProRoot MTA on human periodontal ligament stem cells
    (2019-01-25) Rodríguez Lozano, Francisco Javier; Oñate Sánchez, Ricardo Elías; Collado-González, Mar; López-García, Sergio; García Bernal, David; Tomás Catalá, Christopher Joseph; Moraleda Jiménez, José María; Lozano, Adrián; Forner, Leopoldo; Dermatología, Estomatología, Radiología y Medicina Física
    Objective The aim of this study was to analyze the biological effects of MTA Repair HP and ProRoot MTA on human periodontal ligament stem cells (hPDLSCs) after exposure to acidic and neutral environments. Materials and methods Discs of each material (n = 30) were exposed to phosphate buffered saline (pH = 7.4) or butyric acid (pH = 5.2) for 7 days, and biological testing was carried out in vitro on hPDLSCs. Cell viability and apoptosis assays were performed using eluates of each root-end filling material. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. The chemical composition of the rootend filling materials was determined by energy-dispersive x-ray and eluates were analyzed by inductively coupled plasma-mass spectrometry. Statistical differences were assessed by ANOVA and Tukey test (p < 0.05). Results Under an acidic environment, both materials displayed similar ion release abilities, with the increased release of Si and Ca ions. Substantial changes in microstructure were observed for both materials after exposure to acidic pH. In addition, material exposure to an acidic environment showed a similar degree of cell adherence, and, surprisingly, MTA Repair HP exhibited higher cell viability rates at pH 5.2 than ProRoot MTA. Conclusions Exposure to an acidic environment promoted Si and Ca ion release from ProRoot MTA and MTA Repair HP. Moreover, we observed optimal biological properties of ProRoot MTA and MTA Repair HP in terms of cell viability, cell death, and cell attachment in both environments. Clinical relevance These results may suggest that MTA Repair HP and ProRoot exhibited optimal biological properties in terms of cell viability, cell death and cell attachment in acidic environment, being considered as materials for root-end filling and perforations.
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    Estudio de la biocompatibilidad intraósea de dos materiales dentales de alto contenido en calcio
    (Ergon Ediciones S. L., 2002) Chiva García, Fernando; Carrascosa García, J.; López Jornet, Pía; Ortiz Ruiz, Antonio José; Vicente Ortega, Vicente; Dermatología, Estomatología, Radiología y Medicina Física
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    Melatonin as an agent for direct pulp-capping treatment
    (MDPI, 2020-02-06) Guerrero Gironés, Julia; Alcaina Lorente, Antonia; Ortiz Ruiz, Clara; Ortiz Ruiz, Eduardo; Pecci Lloret, María P.; Rodríguez Lozano, Francisco Javier; Ortiz Ruiz, Antonio José; Martínez Cáceres, Carlos Manuel; Dermatología, Estomatología, Radiología y Medicina Física
    Melatonin plays an essential role in the regulation of bone growth. The actions that melatonin exerts on odontoblasts may be similar to its action on osteoblasts. This research aimed to evaluate the pulp response to melatonin used for direct pulp capping to evaluate the antioxidant effect of melatonin administered orally and its influence on dental pulp. Direct pulp capping was performed on the upper molars of Sprague Dawley rats using melatonin or Mineral Trioxide Aggregate (MTA). The study groups were: MTA; Melatonin; MTA + Melatonin administered orally; and Melatonin + Melatonin administered orally. In the latter two groups, the animals drank water dosed with melatonin ad libitum (10 mg/100 mL). After 30 days, the animals were sacrificed, and 5 ml of blood, the kidneys, and the liver were extracted in order to evaluate oxidative stress using thiobarbituric acid reactive substances testing (TBARS). Fragments of the maxilla containing the study molars were prepared for histological evaluation. The degree of pulp inflammation and pulp necrosis, the presence of reparative dentin and dentin bridging the pulp chamber, the presence and regularity of the odontoblastic layer, and the presence of pulp fibrosis were evaluated. No significant differences were found between the four study groups for any of the studied histological variables. The oral administration of melatonin did not modify the local effects of MTA or melatonin on dental pulp, or reduce basal-level oxidative stress. The effect of melatonin on pulp is similar to that of MTA and may be used as an agent for direct pulp capping.