Browsing by Subject "Limbic system"

Now showing 1 - 6 of 6
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    Internal connectivity of the mouse mesocortical ring and functional implications
    (Springer, 2026-05-08) Puelles López, Luis; García Calero, Elena; Anatomía Humana y Psicobiología; Facultades de la UMU::Facultad de Medicina
    The mammalian mesocortex (MCx) was redefined recently as a complete ring intercalated between the allo- and iso-cortex, attending to the consistent expression of 46 selective gene markers. The MCx exhibits various other characteristics such as low myelin content of its fibers, a property directly related to its function and susceptibility to degenerative disorders. Irrespective of its shared molecular profile, the MCx ring differentiates into different sectors with singular molecular and cytoarchitectonic characteristics, such as the posterior orbitary cortex, the cingulate cortex, and the insula. In the present study, using anterograde connectivity experiments published in an Allen Institute for Brain Science public database (https://brain-map.org/our-research/connectivity), we analyzed the internal connectivity of the mesocortical ring. We observed that its different sectors are multiply interconnected, possibly achieving integrative functions. The medial posterior orbitary cortex stands out; it appears to play a higher hierarchical role within the MCx ring by sending and receiving projections from all mesocortical sectors.
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    Modifications in the distribution of met-enkephalin in the limbic system of the cat brain after electroacupuncture. An immunocytochemical study
    (Murcia : F. Hernández, 1995) Vazquez, J.; Muñoz Madrid, Juan; Cáceres, J.L.
    The distribution of met-enkephalin in the limbic system of the cat brain and its modification after low frequency electroacupuncture (EA) stimulation have been studied experimentally using the indirect immunocytochemistry technique. A marked increase of post-stimulation met-enkephalin immunoreactivity was observed in the tractus habenulo-penduncularis, tractus mamilo-thalamicus, and media1 forebrain bundle, and a decrease at the leve1 of the nucleus interpeduncularis, medialis dorsalis, stria terminals, septalis lateralis, septalis medialis, accumbens septi, supraopticus, and amygdaloideus centralis. The experimental results link the changes in immunoreactivity (and therefore the structures in which they take place) with the action of low frequency EA, and permit the conclusion that the met-enkephalinergic portion of the limbic system studied is directly related morpho-functionally with analgesia and the anatomic pathways of pain.
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    Transcriptomic analysis corroborates the new radial model of the mouse pallial amygdala
    (MDPI, 2025-08-13) Fernández, Gloria; López González, Andrea Lara; Pons-Fuster López, Eduardo; Puelles López, Luis; García Calero, Elena; Anatomía Humana y Psicobiología; Facultad de Medicina
    The mammalian amygdala is located in the temporal lobe of the telencephalon and plays a key role in limbic processing. Recently, our group proposed a radial morphological model to understand the glutamatergic (pallial) part of this nuclear complex in terms of separate progenitor domains. This model explains the amygdala region as consisting of several adjacent developmental radial progenitor units, disposing their distinct periventricular, intermediate, and superficial strata from the ventricle to the pial surface. It was expected that cell populations belonging to specific progenitor domains would present greater molecular similarity to each other than to neighboring developmental units. In this work, we aim to corroborate the existence of several radial domains in the pallial amygdala at the transcriptomic level. snRNAseq experiments in the amygdala of adult mice of both sexes indicated that at low resolution, the whole pallial amygdala was found to divide into two super-radial domains distinguished by differential expression of Slc17a6 and Slc17a7; the former partly imitates molecularly the subpallial (output) amygdalar regions, whereas the rest of the pallial amygdala is molecularly more akin to the surrounding cortical areas. In addition, our snRNAseq transcriptomic analysis fully supports the postulated amygdalar radial model of four main radial domains.
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    Transcriptomics reveals pallial and subpallial subdivisions of the mouse medial amygdala
    (Springer, 2026-02-02) Fernández, Gloria; Puelles López, Luis; Pons-Fuster López, Eduardo; Pla Ferriz, Ramón; García Calero, Elena; Anatomía Humana y Psicobiología; Facultades de la UMU::Facultad de Medicina
    The medial amygdala plays a key role in social brain networks supporting affiliation behavior. This whole domain is widely considered a subpallial (GABAergic) amygdalar subdivision, although it is composed of a mixture of cells of different origins, including populations migrated from the hypothalamus, the prethalamic eminence and the neighboring pallial amygdala. Specifically, the ventral part of the medial amygdala has a high percentage of glutamatergic cells. We developed a transcriptomic study (snRNAseq) to examine the pallial versus subpallial ascription of the mouse medial amygdala cells. We detected high molecular similarity between the ventral region of the medial amygdala and the pallial anterior amygdalar radial unit (classical anterior basomedial nucleus, BMA, and anterior cortical nucleus, ACo), in contrast with the GABAergic profile of the posterodorsal region of the medial amygdala, and the central and intercalated amygdalar nuclei. To verify the possible pallial origin of the ventral medial amygdala, we also analyzed pallial and subpallial marker genes in this region during development and in adult specimens. In addition, we labeled radial glia at perinatal stages to reconstruct the radial domain to which the ventral medial amygdala belongs. The results are consistent with a pallial nature of the ventral medial amygdala, while the posterodorsal medial amygdala is subpallial.
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    Widespread doublecortin expression in the cerebral cortex of the octodon degus
    (Frontiers Media, 2021-04-29) Van Groen, Thomas; Kadish, Inga; Caballero Bleda, María; Baño-Otalora, Beatriz; Rol, María Ángeles; Madrid, Juan Antonio; Popovic, Natalija; Popovic Popovic, Miroljub; Anatomía Humana y Psicobiología
    It has been demonstrated that in adulthood rodents show newly born neurons in the subgranular layer (SGL) of the dentate gyrus (DG), and in the subventricular zone (SVZ). The neurons generated in the SVZ migrate through the rostral migratory stream (RMS) to the olfactory bulb. One of the markers of newly generated neurons is doublecortin (DCX). The degu similarly shows significant numbers of DCX-labeled neurons in the SGL, SVZ, and RMS. Further, most of the nuclei of these DCX-expressing neurons are also labeled by proliferating nuclear antigen (PCNA) and Ki67. Finally, whereas in rats and mice DCX-labeled neurons are predominantly present in the SGL and SVZ, with only a few DCX neurons present in piriform cortex, the degu also shows significant numbers of DCX expressing neurons in areas outside of SVZ, DG, and PC. Many areas of neocortex in degu demonstrate DCX-labeled neurons in layer II, and most of these neurons are found in the limbic cortices. The DCX-labeled cells do not stain with NeuN, indicating they are immature neurons.
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    Widespread doublecortin expression in the cerebral cortex of the Octodon degus
    (Frontiers Media, 2021-04-29) Groen, Thomas van; Kadish, Inga; Caballero Bleda, María; Baño Otalora, Beatriz; Rol de Lama, María de los Ángeles; Madrid, Juan Antonio; Popovic, Natalija; Popovic Popovic, Miroljub; Fisiología
    It has been demonstrated that in adulthood rodents show newly born neurons in the subgranular layer (SGL) of the dentate gyrus (DG), and in the subventricular zone (SVZ). The neurons generated in the SVZ migrate through the rostral migratory stream (RMS) to the olfactory bulb. One of the markers of newly generated neurons is doublecortin (DCX). The degu similarly shows significant numbers of DCX-labeled neurons in the SGL, SVZ, and RMS. Further, most of the nuclei of these DCX-expressing neurons are also labeled by proliferating nuclear antigen (PCNA) and Ki67. Finally, whereas in rats and mice DCX-labeled neurons are predominantly present in the SGL and SVZ, with only a few DCX neurons present in piriform cortex, the degu also shows significant numbers of DCX expressing neurons in areas outside of SVZ, DG, and PC. Many areas of neocortex in degu demonstrate DCX-labeled neurons in layer II, and most of these neurons are found in the limbic cortices. The DCX-labeled cells do not stain with NeuN, indicating they are immature neurons.