Browsing by Subject "Golgi method"

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    Regional adaptation of Müller cells in the chick retina. A Golgi and electron microscopical study
    (Murcia : F. Hernández, 1989) Prada, F. A.; Espinar, A.; Chmielewski, C. E.; Dorado, M. E.; Genís-Gálvez, J. M.
    We report the morphological differences of Müller cells in relation to their topography, using the Golgi method. Müller cells in the central retina are long and slender, with numerous inner prolongations. In the peripheral retina, the morphology of the Müller cells adapts to the reduced thickness of the retina1 layers. In this zone, they are short and have thick inner prolongations which end in a large foot in the internal limiting membrane. In the optic disc margin, Müller cells have a particular morphology characterized by thick, arched prolongations that in general form a glial network between the retina and optic nerve. The ultrastructure of these cells is also described. The results are discussed with respect to the nature of Müller cells.
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    Regional adaptation of Müller cells in the chick retina. A Golgi and electron microscopical study
    (Murcia : F. Hernández, 1989) Prada, F. A.; Espinar, A.; Chmielewski, C. E.; Dorado, M. E.; Genís-Gálvez, J. M.
    We report the morphological differences of Müller cells in relation to their topography, using the Golgi method. Müller cells in the central retina are long and slender, with numerous inner prolongations. In the peripheral retina, the morphology of the Müller cells adapts to the reduced thickness of the retina1 layers. In this zone, they are short and have thick inner prolongations which end in a large foot in the internal limiting membrane. In the optic disc margin, Müller cells have a particular morphology characterized by thick, arched prolongations that in general form a glial network between the retina and optic nerve. The ultrastructure of these cells is also described. The results are discussed with respect to the nature of Müller cells.
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    The Golgi method. A historical through contemporary view
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) González Burgos, Ignacio
    Knowledge regarding the biology of the nervous system and its functions has gone through various theoretical, methodological, and interpretative stages throughout history, depending largely on technical advances that have allowed us not only to approach old questions from new perspectives but also to address new ones. One advance that constituted a watershed in the history of neuroscience was the appearance of a chrome-silver staining technique called the Golgi method that allowed the complete, three-dimensional observation of nerve cells. Discovered by Camilo Golgi and, later, modified significantly and employed by Santiago Ramón y Cajal, Golgi’s method was crucial in demonstrating the veracity of the Neuronal Theory over the earlier Reticular Theory, and in revealing numerous findings related to the human brain and those of many other animal species, which continue to be analyzed today. Despite a period of scientific recession in the first half of the 20th century, the use of the Golgi method prevailed and even expanded in the second half of that century and into the 21st, as researchers continued to use it in its original or modified form and in combination with emerging methodologies. Currently, there are no signs of any decline in its use.