Browsing by Subject "E. coli"

Now showing 1 - 4 of 4
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    Preventive Veterinary Medicine 186 (2021) 105222Available online 25 November 20200167-5877/© 2020 Elsevier B.V. All rights reserved.Resistance patterns to C and D antibiotic categories for veterinary use of Campylobacter spp., Escherichia coli and Enterococcus spp. commensal isolates from laying hen farms in Spain during 2018
    (2021) Rivera Gomis, Jorge; Marín, Pedro; Otal, Julio; Galecio, Juan Sebastián; Martinez Conesa, Cristina; Cubero Pablo, María José; Geografía
    Antimicrobial Resistance (AMR) is a global threat for human and animal health. Few studies have been carried out on laying hens. The aim of this work was to evaluate the antimicrobial susceptibility of commensal Campylobacter spp., E. coli, and Enterococcus spp. isolates in Spanish laying hens in 2018. Samples were collected from 39 laying hen farms. The microorganisms of interest were isolated and confirmed by PCR. The Minimum Inhibitory Concentration (MIC) to antimicrobials of C and D categories were determined. 195 E. coli, 195 Enterococcus spp. and 25 Campylobacter spp. isolates were obtained. E. coli isolates showed high resistance to D category antimicrobials (sulfamethoxazole 76.41 %, tetracycline 62.05 %, trimethoprim 50.77 %, ampicillin 30.77 %) and lower resistance to C category (azithromycin 30.26 %, gentamicin 12.31 %, chloramphenicol 4.62 %). A 10.26 % of E. coli isolates were susceptible to all antimicrobials tested, Multi Drug Resistance (MDR) to 3 antimicrobial families was found in 23.08 % of the isolates and 13.85 % were MDR to 4 families, being Erythromycin-Sulfamethoxazole-Tetracycline the most common resistance profile (10.77 %). Enterococcus spp. showed very high resistance to D category tetracycline (78.47 %) and C category erythromycin (76.42 %). The 11.79 % of Enterococcus spp. isolates were susceptible to all antimicrobials and 53.33 % were resistant to 2 families, being Erythromycin-Tetracycline the most common AMR profile (51.79 %). Regarding Campylobacter spp., resistance to tetracycline (48 %) was higher than resistance to C category antimicrobials (erythromycin 28 %, streptomycin 24 %, gentamicin 16 %). There was a 52 % sensitivity to all tested antimicrobials and 24 % showed MDR to aminoglycosides, macrolides and tetracyclines (Gentamicin-Streptomycin-Erythromycin-Tetra-cycline MDR profile). Novel data on AMR in laying hen commensal isolates in Spain was provided. High resis-tance to several antimicrobials was found, especially to key drugs for the treatment of zoonosis, which represents a public health risk. Better surveillance and careful regulation of antimicrobial use is required in laying hen production.
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    Regulation of the pyrimidine biosynthetic pathway by lysine acetylation of E. coli OPRTase
    (2022-08-22) Lozano Terol, G.; Gallego Jara, J.; Sola Martinez, R. A.; Ortega Retuerta, A.; Martinez Vivancos, A.; Canovas Diaz, M.; De Diego Puente, María Teresa; Bioquímica y Biología Molecular B e Inmunología
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    Resistencia fenotípica y genotípica en los patotipos AIEC, STEC y EAEC de E. coli
    (Universidad de Murcia. Servicio de publicaciones, 2022) Ruiz Herrero, Eduardo; Caro Vergara, María Rosa; Ortega Hernández, Nieves
    Escherichia coli es una bacteria que está muy presente en nuestro día a día, como comensal o como forma patógena. Hay gran cantidad de cepas patógenas capaces de producir enfermedades, siendo de gran importancia para la salud pública aquellas cepas vehiculadas a través de los alimentos, provocando toxiinfecciones alimentarias con gran morbilidad. En algunos casos, las cepas que provocan estos brotes puedes ser muy patógenas ocasionando una gran mortalidad y con repercusiones muy graves para los afectados. En este estudio nos centramos en investigar la presencia de cepas vehiculadas a través de los alimentos de origen cárnico que formen parte de alguno de los 3 patotipos investigados (STEC, AIEC y EAEC). Por otro lado, planteamos investigar la resistencia a los antibióticos que presentan las cepas de E. coli aisladas en los alimentos cárnicos. Nuestros resultados han evidenciado la elevada resistencia fenotípica que presentan la gran mayoría de las cepas de E. coli aisladas en este estudio, planteando un grave problema para la salud pública. Esto hace necesario extremar las medidas de vigilancia y control de estos patotipos a lo largo de la cadena alimentaria. También es necesario ser cautos en el empleo de antibióticos ya que si se hace un uso inadecuado de los mismos provocaría un aumento en la dificultad terapéutica de las infecciones, amenazando con acabar con un siglo de avances médicos
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    Ultrastructural evidence of the evolutional process in malakoplakia
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Jung, Yeon Seung; Chung, Dong Yong; Kim, Eun Jin; Cho, Nam Hoon
    Context. Malakoplakia can be caused by incomplete digestion of Escherichia coli by lysosomes, leading to recurrent urinary tract infections and consequential mass-forming events that mimic tumors. Objectives. By using ultrastructural findings, we aimed to specify the process of phagolysosome to evoke malakoplakia. Design. We observed a series of processes to form a peculiar Michaelis-Gutmann (MG) body in three patients with malakoplakia and compared with xanthogranulomatous pyelonephritis. Results. The ultrastructural findings were realigned according to the sequence of events as pre-phagosomal, phagosomal, and post-phagosomal stages. For the mature MG body, numerous lysosomal aggregates targeting pathogens and subsequent incomplete digestion are prerequisite factors for the pre-phagosomal stage. Scattered lamellated residue is late evidence of the pre- phagosomal stage. Phagosomes can be formed by the fusion of multiple pathogens and multiple lysosomes. We utilized transmission and scanning electron microscopy to speculate on the process of phagolysosomal formation. Conclusion. The recognition of E. coli captured by phagosomes or partially damaged by lysosomal attack within the cell was recorded for the first time. Furthermore, SEM observation was performed on human tissue