Datos de Investigación

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El objetivo de esta comunidad es poner a disposición de los usuarios aquellos materiales generados o recolectados durante el transcurso de una investigación.

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Now showing 1 - 20 of 30

Open Access
A new approach to study inflammation in fish: Serum proteinogram analysis in gilthead seabream (Sparus aurata) injected with λ-carrageenan
Campos Sánchez, Jose Carlos; Campos Sánchez, José Carlos; Esteban Abad, María Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
Open Access
Bioinformatic and gene expression analysis of the somatostatin/cortistatin gene family in the gilthead seabream (Sparus aurata).
Campos Sánchez, José Carlos; Serna Duque, John Alberto; Guardiola Abellán, Francisco Antonio; Cuesta Peñafiel, Alberto; Esteban Abad, María Ángeles; Biología Celular e Histología
Open Access
Biomarker-based assessment of somatostatin-6 immunomodulation in gilthead Seabream: From serum proteins to skin mucus enzymes
(0022-10-20) Campos Sánchez, José Carlos; Espinosa Ruiz, Cristóbal; Marín Parra, Claudia; Guardiola Abellán, Francisco Antonio; Esteban Abad, María Ángeles; Biología Celular e Histología
This study evaluated the immunomodulatory role of somatostatin-6 (SST6) in gilthead seabream (Sparus aurata) using a validated inflammation model induced by λ-carrageenan and a comprehensive panel of serum and skin mucus biomarkers. SST6 was administered at 1 nM and 2 nM, and its effects were assessed in these matrices. The λ-carrageenan model triggered a local inflammatory response with reduced peroxidase, esterase, and protease activities in skin mucus, consistent with the resolution phase of innate immunity. SST6 at 1 nM enhanced serum complement activity without affecting acute-phase proteins, suggesting reinforcement of basal immune surveillance in the host. When combined with λ-carrageenan, SST6 promoted an increase in serum immunoglobulin levels and a selective shift in proteinogram fractions (α2-globulin), while further decreasing the oxidative and proteolytic activities in skin mucus. Correlation analysis revealed the coordinated regulation of key mucosal enzymes, reinforcing the functional integration of local immune responses. These results indicate that SST6 facilitates an early transition from innate to adaptive immunity while preserving the integrity of the mucosal barrier. Furthermore, this study provides the first evidence of complement activation by SST6 in fish and highlights the value of integrating biomarker-based tools to monitor immunophysiological responses and test bioactive compounds in aquaculture research.
Open Access
Data for compounds included in the article: "Exploring the multifaceted effects of dietary encapsulated piscidin 1 on gilthead seabream (Sparus aurata): from immunomodulation to muscle quality"
(2026-05-22) Marín Parra, Claudia; Valero Cases, Estefanía; Espinosa Ruiz, Cristóbal; Coxon, Christopher R.; Esteban Abad, María Ángeles; Biología Celular e Histología
Open Access
Data of the work entitled: Ability of short-chain fatty acids to reduce inflammation and attract leucocytes to the inflamed skin of gilthead seabream (Sparus aurata L.)
Albaladejo Riad, Nora; El qendouci, Mouna; Cuesta Peñafiel, Alberto; Esteban Abad, María Ángeles; Biología Celular e Histología
Restricted
Data set of Novel hepcidin genes in Gilthead Seabream: implications for immune response and iron metabolism
(2025-04-24) García Navarro, Laura; Serna Duque, John Alberto; Cuesta Peñafiel, Alberto; Esteban Abad, María Ángeles; Biología Celular e Histología
Results of Synteny and phylogeny analysis of hamp2.0 and hamp2.15 in the class Actinopterygii, Bactericidal activity of HAMP2α or HAMP2Ω, Cytotoxicity activity of HAMP2α or HAMP2Ω. And a QuantStudio™ 5 Real-Time PCR System (Applied Biosystems).
Open Access
Dataset used for the article: "Temporal dynamics of inflammation in gilthead seabream (Sparus aurata) induced by λ-carrageenan, LPS, and Poly I:C: from behaviour to gene expression"
(2024-07-11) Campos Sánchez, José Carlos; Cabrera Álvarez, María José; Oliveira, Ana Rita; Duarte Oliveira, Gonçalo; Soares, Florbela; Saraiva , João L.; Esteban Abad, María Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
Open Access
Dataset used for the study entitled: JUNO-Coated Beads as a Functional Assay to Capture and Characterize Fertilization-Competent Human Sperm
(2026) Cots Rodríguez, Paula; Wang, Xinyin; Siu, Karen K.; Yip, Patrick; Gómez Sánchez, Emilio; Lee. Jeffrey E.; Hamze Araujo, Julieta Gabriela; Jíménez Movilla, María; Sánchez Tudela, Mirian; Biología Celular e Histología; Facultades de la UMU::Facultad de Medicina
Open Access
Effect of silk fibroin microparticles on cellular immunity and liver of gilthead seabream (Sparus aurata L.) with and without experimental skin injuries
(Elsevier, ) Albaladejo Riad, Nora; Espinosa Ruiz, Cristóbal; Esteban Abad, María Ángeles; Biología Celular e Histología
Silk fibroin microparticles (SF) were administered in the diet of gilthead seabream with or without experimental skin wounds to study the effects on cellular immunity and liver. A commercial diet was enriched with different SF contents: 0, 50 and 100 mg kg-1 (control, SF50 and SF100 diet, respectively). The animals were fed for 30 days and half of them were sampled. Similar experimental wounds were then performed to the rest of fish and they continued to be fed the same diet. At 7 days post-wounding, samples were taken from the wounded fish. Cellular immunity was studied on head kidney leucocytes (phagocytosis, respiratory and peroxidase content) and liver status (histological study and gene expression) were studied. Our results showed that experimental wounds affect both cellular immunity (by decreasing leucocyte respiratory burst and peroxidase activity) and altered liver histology (by inducing vascularization and congestion of blood vessels). In addition, it affects the expression of genes indicative of oxidative stress, endoplasmic reticulum stress and apoptosis. More specifically, it was the highest dose of silk fibroin (SF100) that significantly increased the phagocytic capacity of leucocytes as well as vascularization in the liver. In addition, increased expression of antioxidant genes (cat and gsr) and decreased expression of genes related to reticulum endoplasmic stress (grp94 and grp170) and apoptosis (nos and jnk) were detected in these fish fed with SF100 and wounded. In conclusion, fed fish with SF100 had many beneficial effects as cellular immunostimulant and hepatoprotection in wounded fish. Its use could be of great interest for stress management in farmed fish conditions.
Open Access
Effects of cantharidin on fish erythrocytes, tumour cell lines, and marine pathogenic bacteria
(2023-04) Campos Sánchez, José Carlos; Esteban Abad, María Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
Cantharidin, a toxic monoterpene secreted by blister beetles, has long been used in traditional Chinese and modern medicine for its unique properties. However, despite its widespread use, its effects on fish have not been studied in depth. The aim of this study was to evaluate the potential therapeutic applications of cantharidin in fish by examining its antioxidant, hemoagglutinating, hemolytic and cytotoxic activities at different concentrations (0, 0.625, 1.25, 2.5, 5 and 10 μg mL-1) in three different cell lines. In addition, the study explored the bactericidal and bacteriostatic properties of cantharidin against various fish pathogenic bacteria. The results revealed that there were no significant differences in antioxidant, hemagglutinating or hemolytic activities between the different concentrations of cantharidin tested. However, the study found that cantharidin exhibited dose- and time-dependent cytotoxicity in sea bream (Sparus aurata) erythrocytes and in SAF-1, PLHC-1 and Hela cell lines, resulting in morphological changes indicative of apoptosis. Interestingly, the highest dose of cantharidin tested demonstrated potent bactericidal activity against four marine fish opportunistic bacteria, including Vibrio harveyi, V. anguillarum, Photobacterium damselae and Tenacibaculum maritimum, but no statistically significant changes in bacteriostatic activity were observed against any of the bacteria tested. Overall, these results provide valuable information on the potential therapeutic applications of cantharidin in fish aquaculture. Further research is needed to fully understand the mechanisms of action and to explore possible preventive uses of cantharidin in fish.
Open Access
Erythrocyte phagocytosis in gilthead seabream (Sparus aurata) and European sea bass (Dicentrarchus labrax).
(2024-11-04) Campos Sánchez, Jose Carlos; Meseguer, José; Esteban Abad, María Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
Open Access
Gene expression raw data of the work entitlled: "evolution of a gilthead sea bream (Sparus aurata L.) caudal fin skin explant"
Albaladejo-Riad, Nora; García-Álvarez, Miguel Ángel; Cuesta Peñafiel, Alberto; Esteban Abad, María Ángeles; Marín Parra, Claudia; Biología Celular e Histología
Open Access
In vitro effects of a natural marine algae polysaccharide (λ-carrageenin) on seabream erythrocytes, tumour cell lines and marine bacterial pathogens
Campos-Sánchez, José Carlos; Esteban Abad, María Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
Carrageenin is a high molecular weight sulphated mucopolysaccharide used to induce experimental inflammation in mammals. In addition, it possesses a wide variety of properties that have not yet been studied in fish. This study evaluated the hemagglutinating, hemolytic, cytotoxic, and antibacterial activities of λ-carrageenin. The results showed that λ-carrageenin has hemagglutinating and hemolytic activities on gilthead seabream erythrocytes, which were dose and time-dependent during the first 6 hours of incubation. No significant effects on the haemolytic activity of erythrocytes were observed after incubation for 12 or 24 h with λ-carrageenin. The PLHC-1 cell line showed significant increases in cytotoxic activity after 6 or 12 hours of incubation compared with control cells, and the highest doses of λ-carrageenin caused cytotoxicity in PLHC-1 cells after 24 h of incubation. The morphology of PLHC1 cells incubated with the highest doses of λ-carrageenin for 12 or 24 h showed obvious cell death changes compared with control cells. Interestingly, no significant variations in cytotoxic activity were observed in SAF-1 or HeLa cell lines after incubation with λ-carrageenin. λ-carrageenin showed significant dose-dependent bactericidal activity against Photobacterium damselae but had no significant effect on the bactericidal activity of Vibrio harveyi, V. anguillarum, and Tenacibaculum maritimum. The study suggests that λ-carrageenin has potential applications in aquaculture and aquatic pharmaceutical industries as a hemagglutinating, hemolytic, and antibacterial agent.
Open Access
In vitro immune-depression and anti-inflammatory activities of cantharidin on gilthead seabream (Sparus aurata) leucocytes activated by λ-carrageenan
Campos-Sánchez, Jose Carlos; Guardiola Abellán, Francisco Antonio; Esteban Abad, María Ángeles; Biología Celular e Histología
Cantharidin is a natural compound with known therapeutic applications in humans. However, its properties have hardly been studied in fish. The aim of this study was to investigate the in vitro effects of cantharidin on gilthead seabream (Sparus aurata) head kidney leucocytes (HKL) stimulated with λ-carrageenan. Thus, HKLs were incubated for 24 h with cantharidin (0, 2.5 and 5 μg mL-1) and λ-carrageenan (0 and 1,000 μg mL-1) and cell viability and morphology, immunological parameters and several selected genes related to inflammation and apoptosis were evaluated. The results showed that cantharidin only decreased HKL viability when incubated with the highest dose (5 μg mL-1) and in combination with λ-carrageenan. As for the immunological parameters studied, cantharidin increased the peroxidase activity of HKLs only when incubated in combination with λ-carrageenan, but inhibited the respiratory burst and phagocytic activities even after being previously increased by λ-carrageenan. Furthermore, cantharidin induced morphological changes in HKLs with apoptotic and vacuolization signs that were enhanced when incubated in combination with λ-carrageenan. Considering the analysis of gene expression in HKLs, although λ-carrageenan up-regulated the expression of the proinflammatory gene il1b, cantharidin down-regulated its expression in HKLs exposed to both λ-carrageenan and cantharidin. In addition, cantharidin up-regulated casp3 and casp9 expression, which was down-regulated in HKLs incubated with both molecules. This combination also up-regulated casp1 expression. The present results provide detailed insight into the immune-depressive and anti-inflammatory properties of cantharidin on immune cells, which could be of interest to the aquaculture sector.
Restricted
Metabolomic profiling of the serological response to a hepcidin 1 injection in gilthead seabream (Sparus aurata)
García Navarro, Laura; Esteban Abad, María Ángeles; Marín Parra, Claudia; Biología Celular e Histología
Datos obtenidos mediante HILIC y RP con ESI positiva y negativa.
Open Access
Mitochondrial metabolism characterization of four different fish cell lines
Espinosa-Ruiz, Cristóbal; Mayor-Lafuente, Javier; Esteban Abad, María Ángeles; Biología Celular e Histología
Different cell lines (SAF-1, DLB-1, PLHC-1 and FuB-1) mitro stress (OCR and ECAR) raw data.
Open Access
Participation of hepcidins in the inflammatory response triggers by λ-carrageenin in gilthead seabream (Sparus aurata)
Campos-Sánchez, José Carlos; Serna-Duque, Jhon A.; Alburquerque, Carmen; Esteban Abad, María Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
The role of hepcidins, antimicrobial peptides involved in iron metabolism, immunity, and inflammation is studied. First, gilthead seabream (Sparus aurata L.) head-kidney leucocytes (HKLs) were incubated with λ-carrageenin to study the expression of hepcidin and iron metabolism-related genes. The expression of most of the genes studied was up-regulated, whereas the ferroportin gene (slc40a) was down-regulated in HKLs incubated with λ-carrageenin. In the second part of the study, seabream specimens were injected with λ-carrageenin or buffer (control). The expression of the same genes was evaluated in the head kidney, liver, and skin at different time points after injection. The expression of Hamp1m, ferritin b and ferroportin genes (hamp1, fthb, and slc40a) was up-regulated in the head kidney of fish from the λ-carrageenin group, while the expression of Hamp2C and Hamp2E genes (hamp2.3 and hamp2.7) was down-regulated. In the liver, the expression of hamp1, ferritin a (ftha), slc40a, Hamp2J and Hamp2D (hamp2.5/6) genes was down-regulated in the λ-carrageenin group. In the skin, the expression of hamp1 and (Hamp2A Hamp2C) hamp2.1/3/4 genes was up-regulated in the λ-carrageenin group. A bioinformatic analysis was performed to predict the presence of transcription factor binding sites in the promoter region of hepcidins. Structural and physicochemical variations were found in the different mature hepcidin peptides. This study sheds light on the poorly understood roles of hepcidins in fish. The results provide insight into the regulatory mechanisms of inflammation in fish and could contribute to the development of new strategies for treat inflammation in farm animals.
Open Access
Radiation shielding potential of Oyster Shells (Ostrea edulis)
(2026-07-27) Gómez, Cristina; Esteban Abad, María Ángeles; Martínez López, Salvadora; Arnaldos Sanabria, María Isabel; Esteban Abad, María Ángeles; Biología Celular e Histología; Facultades de la UMU::Facultad de Biología
El objetivo de este estudio era analizar la composición elemental y la mineralogía de las conchas de ostras de cultivo (O. edulis, Linnaeus, 1758) y explorar la viabilidad de estas conchas para el desarrollo de biomateriales de protección contra la radiación. Las conchas pueden valorizarse, sustituyendo parcialmente a las fuentes minerales no renovables en algunas aplicaciones actuales, contribuyendo así a la implantación de una economía circular de cero residuos. Además, el uso de carbonato cálcico (CaCO₃) procedente de las conchas en aplicaciones que garanticen su inertización durante largos periodos puede evitar la liberación de dióxido de carbono a la atmósfera, contribuyendo así a la reducción de la huella de carbono de la industria del marisco.
Restricted
Raw quantitative proteomics dataset of the membrane fractions of bovine and mouse oocytes and zygotes.
(2025) Jíménez Movilla, María; Hamze Araujo, Julieta Gabriela; Biología Celular e Histología
Open Access
Reduced hepcidin 1 of gilthead seabream (Sparus aurata): Immunomodulatory effects and implications in iron homeostasis
(2026) Marín Parra, Claudia; García, Laura; Serna Duque, John Alberto; Ortiz García, María; Martínez López, Salvadora; R. Coxon, Christopher; Espinosa Ruiz, Cristóbal; Esteban Abad, María Ángeles; Esteban Abad, María Ángeles; Biología Celular e Histología; Facultades de la UMU::Facultad de Biología

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Browsing Datos de Investigación by browse.metadata.contributordepartment "Biología Celular e Histología"

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