Investigación
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Investigación contiene los documentos generados por los docentes e investigadores de la Universidad en su labor de investigación. Este depósito tiene como objetivos el almacenamiento, la gestión, la preservación y la difusión de estos documentos, y pretende contribuir al aumento de la visibilidad del trabajo desarrollado por los miembros de la comunidad universitaria.
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Browsing Investigación by browse.metadata.contributordepartment "Biología Celular e Histología"
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- PublicationOpen Access17alpha-ethynylestradiol prevents the natural male-to-female sex change in gilthead seabream (Sparus aurata L.)(Springer Nature, 2020-11) Cabas, I.; Rodenas, M.C.; Arizcun, M.; Chaves-Pozo, E.; Power, D.M.; García Ayala, Alfonsa; García Hernández, María del Pilar; Biología Celular e Histología
- PublicationOpen Access17α-Ethynylestradiol alters the peritoneal immune response of gilthead seabream(Elsevier, 2017) Gómez González, Nuria Esther; Cabas, Isabel; Rodenas, María del Carmen; Arizcun, Marta; García Ayala, Alfonsa; Mulero Méndez, Victoriano Francisco; Biología Celular e Histología17α-Ethynylestradiol (EE2), a synthetic estrogen used in most oral contraceptives pills and hormone replacement therapies, is found in many water bodies, where it can modulate the fish immune response. EE2 acts as an endocrine disruptor in gilthead seabream, Sparus aurata L., a marine teleost fish of great economic value in Mediterranean aquaculture, as it induces hepatic vitellogenin gene (vtg) expression. Moreover, EE2 also alters the capacity of gilthead seabream to appropriately respond to infection although it does not behave as an immunosuppressor. Nevertheless, these previous studies have mainly focused on the head kidney leukocytes and no information exists on peritoneal leukocytes, including mast cells. In the present work, juvenile gilthead seabream fish were fed a pellet diet supplemented with EE2 for 76 days and intraperitoneally injected with hemocyanin plus imject alum adjuvant at the end of EE2 treatment and 92 days later, and the peritoneal immune response was analyzed. EE2 supplementation induced vtg expression but returned to basal levels by 3 months post-treatment. Interestingly, gilthead seabream peritoneal leukocytes express the genes encoding for the nuclear estrogen receptor α and the G protein-coupled estrogen receptor 1 and the dietary intake of EE2 induced these expression. Moreover, EE2 induced an inflammatory response in the peritoneal cavity in unvaccinated fish, which was largely maintained for several months after the cessation of the treatment. However, the impact of EE2 in vaccinated fish was rather minor and transient. Taken together, the study provides fresh information about endocrine immune disruption, focusing on peritoneal leukocytes.
- PublicationOpen AccessA simple format feed to test the acceptability of ingredients for common octopus (Octopus vulgaris Cuvier, 1797)(Wiley, 2015-03-10) Cerezo Valverde, Jesús; García García, Benjamín; Sánchez Morillo-Velarde, María Piedad; Biología Celular e Histología
- PublicationOpen AccessAluminum is a powerful adjuvant in teleost fish despite failing to induce interleukin-1β release(2018-08) Angosto, Diego; López-Muñoz, Azucena; García-Alcázar, Alicia; Meseguer, José; Sepulcre Cortés, María Pilar; Sepulcre Cortés, María Pilar; Biología Celular e Histología
- PublicationOpen AccessAn infrared investigation in relation with chitin and chitosan characterization(Elsevier, 2001-01-12) Brugnerotto, J.; Lizardi, J.; Argüelles Monal, Waldo; Desbrières, J.; Rinaudo, Marguerite; Goycoolea Valencia, Francisco Martín; Biología Celular e HistologíaThe use of infrared spectroscopy for characterization of the composition of chitin and chitosan covering the entire range of degree of acetylation (DA) and a wide variety of raw materials is examined further. The ratio of absorbance bands selected was calibrated using 1H liquid and 13C CP-MAS solid-state NMR as absolute techniques. IR spectra of the structural units of these polymers validated the choice of baselines and characteristic bands. The bands at 1650 and 1320 cm-1 were chosen to measure the DA. As internal reference, the intensities at 3450 and 1420 cm-1 were evaluated. The absorption band ratios involving the reference at 3450 cm-1 had poorer fit. The absorption ratio A1320/A1420 shows superior agreement between the absolute and estimated DA-values (DA% = 31.92A1320/A1420 - 12.20; r = 0.990)
- PublicationOpen AccessAnalysis of ZP1 gene reveals differences in zona pellucida composition in carnivores(2017) Leza, A.; Chevret, P.; González-Brusi, L.; López-Béjar, M.; Ballesta, J.; Izquierdo Rico, María José; Avilés Sánchez, Manuel; Moros Nicolás, Carla; Guillén Martínez, Ascensión; Biología Celular e Histología
- PublicationRestrictedAntiseizure medication for brain metastasis-related epilepsy: Findings of optimal choice from a retrospective cohort(Elsevier, 2021-11-19) Sánchez-Villalobos, José Manuel; Aledo-Serrano, Ángel; Serna-Berna, Alfredo; Salinas-Ramos, Juan; Martínez Alonso, Emma; Pérez-Vicente, José Antonio; Alcaraz Baños, Miguel; Biología Celular e Histología; Facultad de MedicinaObjective: To analyze the prevalence of antiseizure medication (ASM) in patients with brain metastasis-related epilepsy (BMRE) treated with radiosurgery and the relationship between ASM and psychiatric comorbidity. Material and methods: This is a cross-sectional observational design study with retrospective review of medical records of all patients with brain metastases treated with volumetric modulated arc therapy radiosurgery (VMAT-RS) between 2012 and 2018 in a tertiary oncology center. We included those patients with BMRE, analyzing the clinical and demographic data, with special attention to psychiatric comorbidities and the use of ASM. Results: Of the 121 patients with brain metastases included for treatment with VMAT-RS, a total of 38 presented BMRE. The most widely used ASM as first-line treatment was levetiracetam (89%). Only 8% of the patients received sodium channel blockers. The most common psychiatric comorbidity was depression (42.1%). Conclusions: Levetiracetam is the most widely used ASM in patients with BMRE treated with VMAT-RS. Nevertheless, common psychiatric comorbidities in this population might change the decision-making of ASM choice.
- PublicationOpen AccessAptamer-Target-gold nanoparticle conjugates for the quantification of fumonisin B1.(MDPI, 2021-01-08) Vicente A Mirón-Mérida; Yadira González-Espinosa; Yun Yun Gong; Yuan Gong; Collado-González, Mar; Goycoolea Valencia, Francisco Martín; Biología Celular e Histología; Facultades de la UMUFumonisin B1 (FB1), a mycotoxin classified as group 2B hazard, is of high importance due to its abundance and occurrence in varied crops. Conventional methods for detection are sensitive and selective; however, they also convey disadvantages such as long assay times, expensive equipment and instrumentation, complex procedures, sample pretreatment and unfeasibility for on-site analysis. Therefore, there is a need for quick, simple and affordable quantification methods. On that note, aptamers (ssDNA) are a good alternative for designing specific and sensitive biosensing techniques. In this work, the assessment of the performance of two aptamers (40 and 96 nt) on the colorimetric quantification of FB1 was determined by conducting an aptamer–target incubation step, followed by the addition of gold nanoparticles (AuNPs) and NaCl. Although MgCl2 and Tris-HCl were, respectively, essential for aptamer 96 and 40 nt, the latter was not specific for FB1. Alternatively, the formation of Aptamer (96 nt)–FB1–AuNP conjugates in MgCl2 exhibited stabilization to NaClinduced aggregation at increasing FB1 concentrations. The application of asymmetric flow field-flow fractionation (AF4) allowed their size separation and characterization by a ultidetection system (UV-VIS, MALS and DLS online), with a reduction in the limit of detection from 0.002 g/mL to 56 fg/mL.
- PublicationOpen AccessAssessing chemical toxicity of ionic liquids on Vibrio fischeri: Correlation with structure and composition(Pergamon – Elsevier Science Ltd, 2016-04-11) Hidalgo, Juana M; García Montalbán, Mercedes; Salinas Hidalgo, María Dolores; Collado-González, Mar; Díaz Baños, F. Guillermo; Víllora Cano, Gloria; Biología Celular e HistologíaOne of the most important properties of the ionic liquids (ILs) is their non-volatility. However, they are wide soluble in water. For this reason, they can be released to aquatic ecosystems and to contribute to water pollution. Nevertheless, toxicological data related to ionic liquids is scarce in literature because of the great number of possible ionic liquids synthesized. The present work reports the toxicity of twenty-nine imidazolium-, pyridinium- and ammonium-based ionic liquids towards the luminescent bacteria Vibrio fischeri. Some of the effects analyzed on the toxicity have been the type of anion, the length of the alkyl chain of the cation, the cation core and the presence of a functionalized side chain in the cation. These results have showed that the main influence on the toxicity of the ILs is the alkyl chain length. A Quantitative Structure-Activity Relationships (QSAR) method has been used to validate our results obtaining a very good agreement.
- PublicationOpen AccessAssociative phenomena in galactomannan-deacetylated xanthan systems(Elsevier, 2001-09-30) Milas, Michel; Rinaudo, Marguerite; Goycoolea Valencia, Francisco Martín; Biología Celular e HistologíaThe interaction between mesquite seed galactomannan (MSG; D-mannose to D-galactose ratio (M/G) ~1.1) and deacetylated xanthan (DX) in 5 mM NaCl leading to synergistic gel formation at 25 °C was investigated and compared with the far more studied system made of xanthan and locust bean gum (LBG; M/G ~3.5). Rheology and differential scanning calorimetry were used to measure temperatures of gel formation and transition enthalpy as a function of polymer composition, while circular dichroism was used to probe the conformation of DX in the LBG– DX system. MSG and DX associate at 25 °C with a well defined stoichiometry of 0.6:1.0 (w/w) at low ionic strength favouring the disordered coil state of DX. When LBG was used in place of MSG in water or 5 mM NaCl, two types of mechanisms of interpolymeric association are envisaged.
PublicationOpen AccessBeneficial roles of feed additives as immunostimulants in aquaculture: a review(2018) Dawood, Mahmoud A.O.; Koshio, Shunsuke; Esteban Abad, María Ángeles; Biología Celular e HistologíaThe use of antibiotics in the aquafeeds to mitigate infectious diseases or to boost growth performance is commonly practiced. Recently, the prophylactic use of antibiotics and chemotherapies have been criticized which eventually led to their ban in aquaculture by law on many countries. In view of the current restrictions on antibiotic use, there is a critical need to evaluate other possible alternatives. One potential substitute for antibiotics in aquafeeds is the use of functional feed additives. The present review is a comprehensive and an updated compilation of the available works on growth promoters and novel feed supplements, their examples, modes of action and useful applications for improving aquafeeds. It highlights probiotics, prebiotics, synbiotics, immunostimulants, organic acids, nucleotides and medicinal herbs. Apart from boosting aquafeeds and safeguarding general health of aquatic animals, some of these substances have been found to possess beneficial immunostimulant and anti-stress relieving properties. Furthermore, the use of these natural substances increases the consumer confidence of farmed fish. Due care has been taken to cover the ongoing trends and recent advances with a perspective vision and their holistic usages and beneficial applications in aquatic animals’ systems. The current study also shed light regarding the management and production aspects of aquatic animals which will enlighten farmers and producers for better economic growth.- PublicationOpen AccessBiocompatibility of new pulp-capping materials NeoMTA Plus, MTA Repair HP and Biodentine on human dental pulp stem cells(Elsevier Science, 2017-11-01) Tomás Catalá, Christopher Joseph; Collado-González, Mar; García Bernal, David; Oñate Sánchez, Ricardo Elías; Forner, Leopoldo; Llena, Carmen; Lozano, Adrián; Moraleda Jiménez, José María; Rodríguez Lozano, Francisco Javier; Biología Celular e HistologíaIntroduction: The aim of the present study was to evaluate the in vitro cytotoxicity of MTA Repair HP, Neo-MTA Plus, and Biodentine, new bioactive materials used for dental pulp capping, on human dental pulp stem cells (hDPSCs). Methods: Biological testing was carried out in vitro on hDPSCs. Cell viability and cell migration assays were performed using eluates of each capping material. To evaluate cell morphology and cell attachment to the different materials, hDPSCs were directly seeded onto the material surfaces and analyzed by scanning electron microscopy. The chemical composition of the pulp-capping materials was determined by energy-dispersive X-ray and eluates were analyzed by inductively coupled plasma-mass spectrometry. Statistical differences were assessed by analysis of variance and Tukey test (P < .05). Results: Cell viability was moderate after 24 and 48 hours in the presence of MTA Repair HP and NeoMTA Plus, whereas at 48 and 72 hours, Biodentine showed higher rates of cell viability than MTA Repair HP and NeoMTA Plus (P < .001). A cell migration assay revealed adequate cell migration rates for MTA Repair HP and NeoMTA Plus, both similar to the control group rates, meanwhile the highest cell migration rate was observed in the presence of Biodentine (P < .001). Scanning electron microscope studies showed a high degree of cell proliferation and adhesion on Biodentine disks but moderate rates on MTA Repair HP and NeoMTA Plus disks. Energydispersive X-ray pointed to similar weight percentages of C, O, and Ca in all 3 materials, whereas other elements such as Al, Si, and S were also found. Conclusions: The new pulp-capping materials MTA Repair HP, NeoMTA Plus, and Biodentine showed a suitable degree of cytocompatibility with hDPSCs, and good cell migration rates, although Biodentine showed higher rates of proliferation time-dependent.
- PublicationOpen AccessBoar sperm motility is modulated by CCK at a low concentration of bicarbonate under capacitation conditions(2023) López-Úbeda, R.; Luongo, C.; Sòria-Monzó, P.; Abril-Sánchez, S.; Grudzinska, P.; García-Vázquez, F.A.; Izquierdo Rico, María José; Avilés Sánchez, Manuel; Moros Nicolás, Carla; Biología Celular e Histología
- PublicationOpen AccessCarbamazepine, cadmium chloride and polybrominated diphenyl ether-47, synergistically modulate the expression of antioxidants and cell cycle biomarkers, in the marine fish cell line SAF-1(Elsevier, 2019-11-21) Espinosa Ruiz, Cristóbal; Manuguerra, Simona; Curcuraci, Eleonora; Santulli, Andrea; Messina, Concetta M.; Biología Celular e HistologíaA wide range of contaminants, industrial by-products, plastics, and pharmaceutics belonging to various categories, have been found in sea water. Although these compounds are detected at concentrations that might be considered as sub-lethal, under certain conditions they could act synergistically producing unexpected effects in term of toxicity or perturbation of biochemical markers leading to standard pathway. In this study, the Sparus aurata fibroblast cell line SAF-1, was exposed to increasing concentrations of carbamazepine (CBZ), polybrominated diphenyl ether 47 (BDE-47) and cadmium chloride (CdCl2) until 72 h, to evaluate the cytotoxicity and the expression of genes related to antioxidant defense, cell cycle and energetic balance. In general, both vitality and gene expression were affected by the exposure to the different toxicants, in terms of antioxidant defense and cell cycle control, showing the most significant effects in cells exposed to the mixture of the three compounds, respect to the single compounds separately. The synergic effect of the compounds on the analyzed biomarkers, underlie the potential negative impact of the contaminants on health of marine organisms.
- PublicationOpen AccessChanges in lipid composition of different tissues of common octopus (Octopus vulgaris) during short-term starvation(Wiley, 2013-07-09) Cerezo Valverde, Jesús; Serra Llinares, Rosa M.; García García, Benjamín; Sánchez Morillo-Velarde, María Piedad; Biología Celular e HistologíaIn this work, we study the variations in concentration (mg 100 g−1 dry weight) and total content (mg individual−1) of different lipid classes in muscle and the digestive gland of Octopus vulgaris during an 8-day starvation period. In all the samples analysed, polar lipids (PL) predominated in muscle (267.3–337.2 mg 100 g−1) compared with neutral lipids (66.9–104.7 mg 100 g−1). A significant positive tendency was observed in muscle for the content and concentration of monoglycerides as a consequence of starvation (P < 0.05). Furthermore, a higher cholesterol (CHO) content was detected in this tissue after the fourth day of starvation compared with the fed animals (P < 0.05). Neutral lipids predominated in the digestive gland (12 958–14 151 mg 100 g−1) compared with PL (3157–6517 mg 100 g−1), with triglycerides, free fatty acids and monoglycerides being the major lipid classes. The concentration of PL and monoglycerides showed a positive trend with starvation, while the triacylglycerol showed a negative tendency (P < 0.05). The results suggest that triglycerides and CHO are transported simultaneously from the digestive gland to the muscular tissues during starvation and the preferential use of PL and CHO during growth phases. It was estimated that lipids contributed 26% of the energy costs of the animals during starvation, mainly in the form of triglycerides from the digestive gland.
- PublicationOpen AccessCharacterisation of the interaction among oil-in-water nanocapsules and mucin(MDPI, 2020-07-28) Collado-González, Mar; Kaur, Gurmeet; González-Espinosa, Yadira; Brooks, Rebecca; Goycoolea Valencia, Francisco Martín; Biología Celular e HistologíaMucins are glycoproteins present in all mucosal surfaces and in secretions such as saliva. Mucins are involved in the mucoadhesion of nanodevices carrying bioactive molecules to their target sites in vivo. Oil-in-water nanocapsules (NCs) have been synthesised for carrying N,N0-(di-m-methylphenyl)urea (DMTU), a quorum-sensing inhibitor, to the oral cavity. DMTU-loaded NCs constitute an alternative for the treatment of plaque (bacterial biofilm). In this work, the stability of the NCs after their interaction with mucin is analysed. Mucin type III from Sigma-Aldrich has been used as the mucin model. Mucin and NCs were characterised by the multi-detection symmetrical flow field-flow fractionation technique (AF4). Dynamic light scattering (DLS) and z-potential analyses were carried out to characterise the interaction between mucin and NCs. According to the results, loading DMTU changes the conformation of the NC. It was also found that the synergistic interaction between mucin and NCs was favoured within a specific range of the mucin:NC ratio within the first 24 h. Studies on the release of DMTU in vitro and the microbial activity of such NCs are ongoing in our lab.
- PublicationOpen AccessChitosan as stabilizing agent for negatively charged nanoparticles(Elsevier, 2016-12-24) Collado-González, Mar; García Montalbán, Mercedes; Peña-García, Jorge; Pérez-Sánchez, Horacio; Víllora Cano, Gloria; Díaz Baños, F. Guillermo; Biología Celular e HistologíaChitosan is a biocompatible polysaccharide with positive Z potential which can stabilize negative charged nanoparticles. Silk fibroin nanoparticles and citrate gold nanoparticles, both with negative Z potential, but they form aggregates at physiological ionic strength. In this work, we study the behavior of chitosan in solution when the ionic strength of the medium is increased and how the concentration of chitosan and the proportion of the two components (chitosan and AuNP or SFN) significantly affect the stability and size of the nanocomposites formed. In addition to experimental measurements, molecular modeling were used to gain insight into how chitosan interacts with silk fibroin monomers, and to identify the main energetic interactions involved in the process. The optimum values for obtaining the smallest and most homogeneous stable nanocomposites were obtained and two different ways of organization through which chitosan may exert its stabilizing effect were suggested.
- PublicationOpen AccessChitosan-Alginate Blended Nanoparticles as Carriers for the Transmucosal Delivery of Macromolecules(American Chemical Society, 2009-06-22) Goycoolea Valencia, Francisco Martín; Giovanna Lollo; Carmen Remuñán-López; Fabiana Quaglia; María J. Alonso; Biología Celular e Histología; Facultades de la UMU::Facultad de BiologíaNanoparticles intended for use in the transmucosal delivery of macromolecules were prepared by the ionic gelation of chitosan (CS) hydrochloride with pentasodium tripolyphosphate (TPP) and concomitant complexation with sodium alginate (ALG). The incorporation of a small proportion of ALG of increasing molecular weight (Mw; from 4 to 74 kDa) into the nanoparticles led to a monotonic increase in colloidal size from ∼260 to ∼525 nm. This increase in size was regarded as a consequence of the formation of radually more expanded structures. Insulin, taken as a model peptide, was associated to CS-TPP-ALG nanoparticles with efficiencies in the range of ∼41 to ∼52%, irrespective of the Mw of the ALG incorporated in the formulation. These CS-TPP-ALG nanoparticles exhibited a capacity to enhance the systemic absorption of insulin after nasal administration to conscious rabbits. Interestingly, it was observed that the duration of the hypoglycaemic response was affected by the ALG’s Mw. Briefly, this work describes a new nanoparticulate composition of potential value for increasing nasal insulin absorption.
- PublicationOpen AccessChitosan-based nanocapsules: physical characterization, stability in biological media and capsaicin encapsulation(Springer-Verlag, 2012-05-08) Goycoolea Valencia, Francisco Martín; Angela Valle-Gallego; Rossana Stefani; Bianca Menchicchi; Laurent David; Cyrille Rochas; Manuel J. Santander-Ortega; María J. Alonso; Biología Celular e Histología; Facultades de la UMU::Facultad de BiologíaThis study addresses the effect of the degree of Nacetylation (DA ~1.4–56 %) of chitosan (CS) of low and medium molecular weight (Mw ~9.5–13.2 and ~122–266 kDa, respectively) on the biophysical properties, colloidal stability and encapsulation efficiency of capsaicin (a lipophilic drug currently used in pain therapy) of CS-based nanocapsules (NCs). The average diameter of the NCs varied within the range of ~150–200 nm, but it was slightly higher for NCs comprising high Mw CS than for those with low Mw CS. The zeta potential (ζ) was highly positive and clearly dependent on the Mw of CS, exhibiting a monotonic decreasing trend concomitant with the increase in DA. Both results suggest that Mw and DA of CS have an effect on the disposition of the polysaccharide onto the phospholipidic surface. Synchrotron SAXS studies revealed a monotonic increase in Bragg interplanar distances (from ~55 up to ~67 Å) as a function of the DA, a finding that agrees with previous results that are consistent with the capacity of hydrophobic domains to disturb the crystalline state ofgelled phospholipidic membranes. Colloidal stability studies carried out in cell-culture biological media revealed the determinant role of hydration forces, a short-range repulsive interaction, on the stability of the NCs. Finally, Mw and DA of CS both influenced the encapsulation efficiency of capsaicin, thus showing the effect of the harnessed NCs shell on its capacity to encapsulate lipophilic drugs.
- PublicationOpen AccessChitosan-nanoparticles effects on mucosal immunity: a systematic review(Elsevier, 2022-08-26) Collado-González, Mar; Esteban Abad, María Ángeles; Biología Celular e HistologíaNanoparticles-based treatments is of utmost importance for aquaculture. In this scenario, chitosan-based nanoparticles have been proposed due to the properties of chitosan, which include mucoadhesiveness. Nevertheless, pivotal parameters of chitosan, such as degree of acetylation and molecular weight, are commonly underestimated in the available literature despite the influence they seem to have on the properties of chitosan-based nanoparticles. In this systematic review, the immunomodulator capacity of chitosan nanoparticles used as mucosal vaccines on teleost fish has been evaluated paying special attention to the chitosan properties. Four databases were used for literature search, yielding 486 documents, from which 14 meet the inclusion criteria. Only 21% of the available studies reported properly chitosan properties, which should be improved in future works to generate reproducible data as well as valuable information. To the best of our knowledge, this work objectively compares for the first time, by quantifying the mg of chitosan/g of fish applied in each study, the chitosan nanoparticle preparation and doses applied to fish, as well as the effects of the treatments applied on fish immune status.