http://hdl.handle.net/10201/17721 Wed, 19 Jun 2013 10:10:24 GMT 2013-06-19T10:10:24Z http://hdl.handle.net/10201/18850 Título: A comparison of cyclosporine A and cyclosporine G in a rabbit heterotopic cardiac transplant model: graft outcome and histological findings Autor/es principal/es: Fryer, J.P.; Pascoe, E.A.; Yatscoff, R.W.; Thliveris, J.A. Resumen: Cervical heterotopic heart transplants were performed on 20 male New Zealand white rabbits comprising 4 treatment groups. Animals in each group were injected daily via the marginal ear vein and received one of the following regimes: Cyclosporine A, 10 mglkglday; Cyclosporine G, 15 mglkglday; cremophor-El, 3mllday; or normal saline. Measurement of 24 hour trough blood concentrations revealed no significant differences between the average concentrations of Cyclosporine A and Cyclosporine G. Animals were examined daily and the cervical allografts assessed by palpation for viabilitylrejection. The duration of the study ended for each animal when the graft stopped beating at which time the animals was euthanized and the transplanted heart and native kidneys harvested and processed for light microscopy evaluation of rejection and drug toxicity, respectively. Graft survival in the Cyclosporine A group significantly surpassed that seen in the Cyclosporine G group as well as the control groups, whereas in animals treated with Cyclosporine G, graft survival was not different from controls. In the native kidney, there were no differences in glomerular tuft area or volume density amongst drug-treated or control animals. In contrast, tubule atrophy and interstitial fibrosis were markedly greater in Cyclosporine A-treated vs Cyclosporine Gtreated animals. The results of this study indicate that, whereas Cyclosporine G is less nephrotoxic than Cyclosporine A, given equivalent blood concentrations Cyclosporine A delays rejection of a cardiac allograft significantly longer than Cyclosporine G in this animal species. Tue, 22 Feb 2011 10:32:38 GMT http://hdl.handle.net/10201/18850 2011-02-22T10:32:38Z http://hdl.handle.net/10201/18849 Título: Odontogenic jaw cysts: light and electron microscopical investigations Autor/es principal/es: Domenici Lombardo, L.; Amunni, F.; Bergamini, M.; Romagnoli, P. Resumen: Light and electron microscopy were used to analyze the epithelial lining of odontogenic cysts excised from edentulous regions of the jaws. Clinically, three cases were identified as keratocysts, and 21 cases as cysts other than keratocysts («non-keratocysts~)T. he epithelium of the former was found to achieve keratinization over most of the surface and to never contain mucus secreting cells. The epithelium of the latter appeared to be in part stratified squamous, with cells loosely connected to each other, and in part stratified columnar, with superficial cells connected to each other by tight junctions and secreting mucus. The results suggest that cysts arising from edentulous regions of the jaws may be either keratocysts or cysts with heterogeneous, non-keratinizing epithelium; the content of keratocysts can be formed mainly by shedding of comified epithelial layers, and that of non-keratocysts by mucus secretion from columnar epithelium associated to fluid filtration through non-keratinizing squamous epithelium. Tue, 22 Feb 2011 10:32:37 GMT http://hdl.handle.net/10201/18849 2011-02-22T10:32:37Z http://hdl.handle.net/10201/18848 Título: Retinal pigment epithelial fine structure in the Australian Galah (Eolophus roseicapillus) (Aves) Autor/es principal/es: Braekevelt, C. R.; Richardson, K.C. Resumen: As part of a comparative morphological study, the fine structure of the retinal epithelium (RPE), choriocapillaris and Bruch's membrane (complexus basalis) has been investigated by light and electron microscopy in the galah (Eolophus I-oseicapillus), an Australian cockatoo. The RPE consists of a single layer of low cuboidal cells joined basally by a series of zonulae occludentes. Basally (sclerally) the retinal epithelial cells display numerous deep infoldings while apically (vitreally) microvillar processes interdigitate with photoreceptor outer segments. Internally the RPE cells show a large vesicular nucleus, plentiful smooth endoplasmic reticulum (SER) and numerous polysomes but very little rough endoplasmic reticulum (RER). Numerous mitochondria are located basally. In the lightadapted specimens studied, the melanosomes of the RPE are almost exclusively located within the apical processes indicating that retinomotor movement of this pigment probably occurs. Phagosomes and lysosomelike bodies are present as are myeloid bodies which may show ribosomes on their outer surface. The choriocapillaris endothelium is thin but only minimally fenestrated facing Bruch's membrane. Most fenestrations of the choriocapillaris display a single-layered diaphragm while the remainder have a double-layered diaphragm. Bruch's membrane (complexus basalis) is typical of avian species in that it is pentalaminate with the central lamina densa displaced towards the choriocapillaris. Tue, 22 Feb 2011 10:32:36 GMT http://hdl.handle.net/10201/18848 2011-02-22T10:32:36Z http://hdl.handle.net/10201/18846 Título: lnitiation and post-initiation chemopreventive effects of O-carotene in toad liver carcinogenesis Autor/es principal/es: Sadek, I.A.; Hayat, L.G. Resumen: Hepatocellular carcinoma were recognized in the toad, Blfo iliridis, in 14 cases out of 50 by injection of 1 mg 7,12 dimethylbenz(a)anthracene (DMBA)/toad twicelweek for 12 weeks. In contrast, toads treated with DMB at the same dose level and B-carotene (BC), 0.05 mg (3 hr prior to the carcinogen)/toad, twicelweek for 12 weeks showed no tumor incidence. However, BC at the same dose level was less effective when administered 3 hr. after the carcinogen (DMBA). In 8 cases out of 50 cases neither tumor growth nor neoplastic changes were observed in toads treated with BC alone or olive oil. It is concluded that BC completely blocked hepatocarcinogenesis in toads when given 3 hr. before initiation. Tue, 22 Feb 2011 10:32:35 GMT http://hdl.handle.net/10201/18846 2011-02-22T10:32:35Z http://hdl.handle.net/10201/18847 Título: Seasonality and freezability vs routine parameters in stallion semen Autor/es principal/es: Rodríguez, H.; Bustos-Obregón, E. Resumen: The fertilizing ability of stallion semen was analyzed using fresh and frozen samples, obtained before (June-July) or during (October-November) the breeding season. Thirty ejaculates obtained from 4 stallions, were used. The analysis comprises routine seminogram; ATP concentration (Comhaire et al., 1983); subjective and objective motility and sperm velocity (Makler, 1980). Freezing was done following the technique of Martin et al. (1979). Sperm velocity, ATP content and objective motility in ejaculates of subjective motility >50% show values of 14.010.84 ym S-l; 4.8f 2 . 7 1~O - ~ Man d 54.0I7.4%, respectively. For ejaculates with subjective motility ~ 5 0 %the~se values are 8.412.4; 0.74f 0 . 3 6 1~ 0 - 7 a~n d 27.0+0.8%. No significant changes in these characteristics were elicited by freezing, though ATP content dropped to 50% after thawing. These characteristics are highly associated between them (pc0.05) and with some conventional parameters of the routine seminogram such as sperm motility, count, total spermatozoal number and morphology. Additionally, sperm chromatin packing was analyzed by decondensing sperm nuclei using a thiol reducing agent. This parameter was not modified by freezing and it may depend on prolonged epididymal sperm storage during the non-breeding season. Epididymal maturation then results in an excessive disulphur bridging in sperm basic proteins, so that hypermaturation with faultly male pronuclear formation can follow. Tue, 22 Feb 2011 10:32:35 GMT http://hdl.handle.net/10201/18847 2011-02-22T10:32:35Z http://hdl.handle.net/10201/18845 Título: A change in the localization of the region trapping immune complexes in rat popliteal lymph nodes during development of germinal centers, with regard to the distribution of follicular dendritic cells Autor/es principal/es: Sato, H.; Dobashi, Michio; Maeda, K. Resumen: A study was conducted to clarify changes in the relationship between the region of irnrnune complex (IC) trapping by follicular dendritic cells (FDCs) and the distribution of FDC during reaction of germinal centers (GCs), and to examine the relationship between the tridiniensional shape of the IC-trapping regions and their two-dimensional shape. Five-week-old rats were given footpad injections of sheep red blood cells, and then their popliteal lymph nodes were excised between days O and 42, 24 h after injection of peroxidase-antiperoxidase complex (PAP) as an IC. The specimens were immunostained for PAP trapping on serial paraffin sections, and for S-100 protein as a marker of FDCs. It was found that during the GC reaction, PAP trapping became weak and then disappeared on the basal side of developing GCs where S-100 protein-positive FDCs were still present. Al1 of the 1933 lymph follicles examined were found to trap PAP. Whereas the tridiniensional shapes of the trapping regions showed similar pattems according to the development of lymph follicles, their two-dimensional shapes varied. We suggest that FDCs in primary follicles may differentiate into FDCs in the light zone and FDCs in the dark zone in secondary follicles. To evaluate each of the compartments of a lymph follicle more accurately, investigators should pay attention to the tridimensional shape of the compartment. Tue, 22 Feb 2011 10:32:34 GMT http://hdl.handle.net/10201/18845 2011-02-22T10:32:34Z http://hdl.handle.net/10201/18844 Título: Use of lectin-probes for correlative histochemical and biochemical assessments of the glycosylation patterns of secretory proteins, including kallikreins, in salivary glands and saliva Autor/es principal/es: Garrett, J.R.; Proctor, G.B.; Zhang, X.S.; Shori, D.K.; Schulte, B.A. Resumen: Labelled lectins were used as probes to study the glycosylation and secretion of submandibular glycoproteins not only in sections of fixed glands but also in glandular extracts and in nerve-induced saliva, after electrophoretic separations and immobilization in nitrocellulose membranes. In cats the glycoproteins in sympathetic saliva differed considerably from those in parasympathetic saliva. In sympathetic saliva they were found to originate mainly from striated ducts, to some extent from demilunar cells and to a small extent from acinar cells, whereas in parasympathetic saliva they arose mainly from acinar-cells añd demilunes and only to a small extent from striated ducts. In rat submandibular glands sympathetic stimulation caused extensive depletion of lectin stainable granules from granular tubules. Corresponding strong binding occurred with the same lectins to constituents in saliva that ran between 25 and 35 kD on SDS gel electrophoresis and were shown to contain tissue kallikreins. Their binding patterns suggested that individual kallikreins from the same gland may be glycosylated in different ways. This possibility was tested on five different kallikreins after separation from submandibular extracts by isoelectric focussing. Lectin bindings on slot blot preparations of these kallikreins were tested before and after N-glycosidase F, sialidase or endo-a-Nacetylgalactosaminidase digestions. Results showed that, despite their close genetic and structural similarities, the kallikreins are in fact differently sialylated and fucosylated and the novel finding that some contain Oglycosidically linked side chains as well as the anticipated N-glycosidically linked side chains was revealed. Thus, correlative histochemical and biochemical Offprint requests to: Professor J.R. Garrett, Secretory and Soft Tissue Research Unit, Department of Oral Pathology, The Rayne Institute, KCSMD, 123 Coldharbour Lane, London, SE5 9NU, England assessments of bindings with lectin probes has provided important new information about differences in the glycosylation pattems of individual glycoproteins stored within the same secretory granules. Tue, 22 Feb 2011 10:32:33 GMT http://hdl.handle.net/10201/18844 2011-02-22T10:32:33Z http://hdl.handle.net/10201/18843 Título: Alterations in the alveolar epithelium after injury leading to pulmonary fibrosis Autor/es principal/es: Kasper, M.; Haroske, G. Resumen: This review discusses current knowledge of the involvement of the alveolar epithelium in tissue remodelling during fibrogenesis. The purpose of the present paper is to give an overview, including the authors' own results, of knowledge of ultrastructural alterations, proliferation kinetics and phenotypic changes of pneumocytes in experimental and clinical pathology of pulmonary fibrosis. After lung injury, the alveolar epithelial cells show ultrastructural alterations, hypertrophy and hyperplasia, and a modulation of a series of structural and membrane proteins such as cytoskeletal changes, loss or de novo expression of epithelial adhesion molecules, and altered lectin binding. Furthermore, enhanced secretion of proteases, of cytokines and other soluble factors can be observed in the alveolar epithelium. These findings suggest the contribution of the epithelium in the remodelling process to be greater than expected. Estimations of the cell kinetics show that type 11 pneumocytes have the proliferative capacity to restore high proportions of damaged type 1 cells within few hours. In fibrosis this capacity also seems to be affected seriously, resulting in transitional phenotypes between type 11 and type 1 cells. Additionally, in the light of the detection of CD44 type of adhesion molecules at the foot processes of type 11 pneumocytes, some aspects of epithelial-fibroblast interaction are described. Tue, 22 Feb 2011 10:32:32 GMT http://hdl.handle.net/10201/18843 2011-02-22T10:32:32Z http://hdl.handle.net/10201/18842 Título: Morphological changes of autoclaved autogenic bone implantation and autoclaved autogenic bone supplernented with allogenic demineralized bone matrix in rat parietal bone Autor/es principal/es: Young, T.Q.; Matsuda, Mikio; Takekawa, M.; Ohtsubo, S.; Tsuyama, K.; Kita, S. Resumen: The healing process of resected, autoclaved (121 7,20 minutes) and re-implanted bone in the rat parietal bone was compared with that of autoclaved bone that was supplemented with allogenic bone matrix (AAA-bone), using a scanning electron microscope and a light microscope. In the implant without AAA-bone, bone union and replacement of the autoclaved bone was seen at 2 weeks after implantation. There was no evidence of any inflammatory reaction around the autoclaved bone. The implant was gradually replaced by the new bone. In the implant with AAA-bone, the new bone formation around the implanted bone was more abundant than that of the implant without AAA-bone. An inflammatory reaction was also observed after 1 week. The replacement of the implant with AAA-bone was inferior to the nonsupplemented group. The reason for the poor replacement was the disturbance of the blood supply in the implant by abundant new bone formation. In these results, the autoclaved bone re-implantation was an excellent bone substitute with osteoconductive ability and biocompatibility. The implantation with AAA-bone was good for the new bone formation, but the position and the technique of supplement with AAAbone have to be more deeply investigated. Tue, 22 Feb 2011 10:32:31 GMT http://hdl.handle.net/10201/18842 2011-02-22T10:32:31Z http://hdl.handle.net/10201/18841 Título: Tenascin and type IV collagen expression in liver cell dysplasia and in hepatocellular carcinoma Autor/es principal/es: Zhao, M.; Laissue, J.A.; Zimmermann, Astrid Resumen: The extracellular matrix (ECM) located in and around tumors is different from normal organ stroma, and there is evidence that it is critically involved in carcinogenesis and malignant growth. Whereas an abnormal composition of ECM in hepatocellular carcinomas (HCC's) has previously been demonstrated, not much is known so far with respect to putative HCC precursor lesions. We have, therefore, systematically analyzed the immunohistochemical reactivity for two major ECM components, tenascin and type IV collagen, in three types of liver cell dysplasia (LCD), and compared the findings with patterns observed in HCC's of different types and grades. Tenascin reactivity was generally stronger in HCC's than in cirrhosis. In cirrhotic nodules harboring areas of LCD, tenascin expression was significantly lower in small cell LCD than in large cell LCD. Type IV collagen reactivity in and around HCC's decreased as a function of a lower differentiation grade. In both groups of cirrhosis, ¡.e. with or without HCC, cirrhotic nodules occupied by the small cell variant of LCD exhibited a significantly lower type IV collagen reactivity than those with large cell LCD or simple regenerative cells. Taken together these findings suggest that, similar to adenomatous hyperplasia, small cell LCD is characterized by an abnormal tenascin and type IV collagen expression, thus reflecting the defective ECM pattern observed in HCC's. Tue, 22 Feb 2011 10:32:30 GMT http://hdl.handle.net/10201/18841 2011-02-22T10:32:30Z